Each tca cycle produces:
Two molecules of carbon dioxide.
Three molecules of NADH.
Three hydrogen ions.
One molecule of FADH₂
One molecule of GTP.
The two carbon atoms that enter the cycle as the acetyl group.
the enzymes of the citric acid cycle are physically associated with one another.
Substrate channeling; Metabolon
The cycle operates only under aerobic conditions, although oxygen does not participate directly in the cycle.
The pyruvate dehydrogenase complex is regulated allosterically and by reversible phosphorylation
TCA cycle is controlled at several points
TCA cycle-Source of Biosynthetic Precursors
The citric acid cycle must be capable of being rapidly replenished if any are drawn off for biosynthesis, to allow the cycle to function
acetyl CoA can not convert into oxaloacetate or any other citric acid cycle intermediate
oxaloacetate can be formed by the carboxylation of pyruvate, a crucial role in gluconeogenesis, which is active only in the presence of acetyl CoA
Pyruvate to Oxaloacetate