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Types of Enteric MediaLevines EMB | -Gram negative Bacilli
-Ferments lactose
-Capable of fermenting glucose
-Would not be chosen as a possible enteric pathogen | -Gram negative bacilli
-Does not ferment lactose (from this info we can’t tell if its capable of fermenting glucose or note
-Uses peptones for growth
-Chosen as a possible enteric pathogen | MacConkey Agar | -Gram negative Bacilli
-Ferments lactose
-Capable of fermenting glucose
-Would not be chosen as a possible enteric pathogen | -Gram negative bacilli
-Does not ferment lactose (from this info we can’t tell if its capable of fermenting glucose or note
-Uses peptones for growth
-Chosen as a possible enteric pathogen | SS Agar | -Gram negative Bacilli
-Ferments lactose
-Capable of fermenting glucose
-Would not be chosen as a possible enteric pathogen | -Gram negative bacilli
-Does not ferment lactose (from this info we can’t tell if its capable of fermenting glucose or note
-Uses peptones for growth
-Chosen as a possible enteric pathogen | XLD | -Gram negative bacilli
-Ferments lactose and or sucrose and or xylose
-Since it ferments at least one of these carbs it is capable of fermenting glucose
-Would not be chosen as a possible enteric pathogen
-H2S is negative | -Gram negative bacilli
-Does not ferment lactose, sucrose or xylose (from this info we can’t tell if its capable of fermenting glucose or note
-Uses peptones for growth
-Chosen as a possible enteric pathogen | HE Agar | -Gram negative bacilli
-Ferments lactose and/or sucrose and/or salicin
-Since it ferments at least one of these carbs it is capable of fermenting glucose
-Would not be chosen as a possible enteric pathogen
-H2S is negative | -Gram negative bacilli
-Does not ferment lactose, sucrose or salicin(from this info we can’t tell if its capable of fermenting glucose or note
-Uses peptones for growth
-Chosen as a possible enteric pathogen
- H2S producing (some colonies are black) |
Differential MediaPurpose | allow differentiation of bacteria based upon some characteristic
- usually based upon s biochemical reaction | pH indicators | register the difference |
Enriched MediaGrowth factors (micronutrients) | blood, carbohydrates, amino acids, vitamins, NaCl | Fastidious bacteria | Need additional nutrients in media or environment | Auxotroph | Bacterium which has mutatued (from the parent prototroph) and developed a specific growth requirement | Halophile | Bacterium needing NaCl in media | Contrast with Enrichment media | - Suppresses normal flora while enhancing growth of pathogens
- Usually for stool specimens |
Selective MediaPurpose | Selects for the growth of some bacteria while inhibiting others | Enteric Media | - Designed to isolate pathogens from the intestine
- All are selective for gram negative bacilli (inhibit gram positives and gram negative cocci)
- Different classifications
-> Differ in their ability to inhibit intestinal normal flora | Low Selectivity | Moderate Selectivity | Highly Selective | Allow ALL gram negative bacilli to grow, whether intestinal pathogen or normal flora | Allow intestinal pathogenic gram negative bacilli to grow while inhibiting some intestinal normal flora gnb | Allow intestinal pathogenic gram negative bacilli to grow while inhibiting virually all intestinal normal flora gnb |
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