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Types of Enteric MediaLevines EMB | -Gram negative Bacilli -Ferments lactose -Capable of fermenting glucose -Would not be chosen as a possible enteric pathogen | -Gram negative bacilli -Does not ferment lactose (from this info we can’t tell if its capable of fermenting glucose or note -Uses peptones for growth -Chosen as a possible enteric pathogen | MacConkey Agar | -Gram negative Bacilli -Ferments lactose -Capable of fermenting glucose -Would not be chosen as a possible enteric pathogen | -Gram negative bacilli -Does not ferment lactose (from this info we can’t tell if its capable of fermenting glucose or note -Uses peptones for growth -Chosen as a possible enteric pathogen | SS Agar | -Gram negative Bacilli -Ferments lactose -Capable of fermenting glucose -Would not be chosen as a possible enteric pathogen | -Gram negative bacilli -Does not ferment lactose (from this info we can’t tell if its capable of fermenting glucose or note -Uses peptones for growth -Chosen as a possible enteric pathogen | XLD | -Gram negative bacilli -Ferments lactose and or sucrose and or xylose -Since it ferments at least one of these carbs it is capable of fermenting glucose -Would not be chosen as a possible enteric pathogen -H2S is negative | -Gram negative bacilli -Does not ferment lactose, sucrose or xylose (from this info we can’t tell if its capable of fermenting glucose or note -Uses peptones for growth -Chosen as a possible enteric pathogen | HE Agar | -Gram negative bacilli -Ferments lactose and/or sucrose and/or salicin -Since it ferments at least one of these carbs it is capable of fermenting glucose -Would not be chosen as a possible enteric pathogen -H2S is negative | -Gram negative bacilli -Does not ferment lactose, sucrose or salicin(from this info we can’t tell if its capable of fermenting glucose or note -Uses peptones for growth -Chosen as a possible enteric pathogen - H2S producing (some colonies are black) |
Differential MediaPurpose | allow differentiation of bacteria based upon some characteristic - usually based upon s biochemical reaction | pH indicators | register the difference |
Enriched MediaGrowth factors (micronutrients) | blood, carbohydrates, amino acids, vitamins, NaCl | Fastidious bacteria | Need additional nutrients in media or environment | Auxotroph | Bacterium which has mutatued (from the parent prototroph) and developed a specific growth requirement | Halophile | Bacterium needing NaCl in media | Contrast with Enrichment media | - Suppresses normal flora while enhancing growth of pathogens - Usually for stool specimens |
Selective MediaPurpose | Selects for the growth of some bacteria while inhibiting others | Enteric Media | - Designed to isolate pathogens from the intestine - All are selective for gram negative bacilli (inhibit gram positives and gram negative cocci) - Different classifications -> Differ in their ability to inhibit intestinal normal flora | Low Selectivity | Moderate Selectivity | Highly Selective | Allow ALL gram negative bacilli to grow, whether intestinal pathogen or normal flora | Allow intestinal pathogenic gram negative bacilli to grow while inhibiting some intestinal normal flora gnb | Allow intestinal pathogenic gram negative bacilli to grow while inhibiting virually all intestinal normal flora gnb |
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