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Oxygen Usage Defini­tions

Obligate Aerobe
Needs O2
-Pseudo­monas, Microc­occus, Bacillus
Facult­ative
Grows with or without O2
-Staph, Strep, Entero­bac­ter­iaceae
Microa­ero­philic
Only requires a small amount of O2
-Borrelia
Obligate Anaerobe
Can't survive in O2
-C. diff

Anaerobic Basics

Basic Reservoirs
-Abscesses
-Body Fluid
-Mouth
-Deep Wound
-Intestine
-UTI
Examples of Infect­ion­s/D­iseases caused by Anaerobes
-Dental Infection
-C.diff
-Deep wound
-Gangrene
-Necrotic Tissue
-Botulism

Aeroto­lerance Testing

1. CDC BA colony plate with growth
2. Sub to regular BA and another CDC BA plate
3. See if they are anaerobes
If they grow on the regular BA and the CDC BA then they are not anaerobes

Anaerobic Basics

Basic Reservoirs
-Abscesses
-Body Fluid
-Mouth
-Deep Wound
-Intestine
-UTI
Examples of Infect­ion­s/D­iseases caused by Anaerobes
-Dental Infection
-C.diff
-Deep wound
-Gangrene
-Necrotic Tissue
-Botulism
 

Specimens

Approp­riate Specimens
Aspirates, body fluid, tissues, suprapubic asspir­ation, drainage, brain abscesses, sinus aspirates
Inappr­opriate (rejected) specimens
Swab of anything, anythying respir­atory b/c of normal flora, vaginal b/c normal flora, urine, stool
Anaerobic Media - All have no oxygen
CDC Blood Agar
Blood Agar without O2
CCFA
Used for C. Diff. Both selective and differ­ential
LKV agar
Laked blood and antibi­otic. Has Vanco and Kanamycin, ONLY selective
Bacter­oides Bile Esculin Agar
Special for Bacter­oides fragilis. Selective and differ­ential (20% bile tolerance)
Thiogl­yco­llate Broth
Broth media to grow bacteria. Can only grow medically signif­icant organisms. Oxygen suppressed
Cooked Meat Glucose Broth
looks like has chunks of beef in it. Has glucose. Does same thing as thiogl­yco­llate broth
Incubation Enviro­nment
Gas Pack Jar
85% N; 10% H; 5% CO2
Anaerobic Chamber

Gram Negative Bacilli

 
Fusoba­cterium necrop­horum
Fusoba­cterium nucleatum
Bile Tolerance (20%
-
-
Brick Red Fluore­scence (UV)
-
-
Brown/­black colonies on CDC
-
-
Vancomycin
R
R
Penicillin
R
R
Kanamycin
S
S
Lipase
+
-
Esculin
-
-

Gram Negative Bacilli

 
Bacter­oides fragilis
Porphy­romonas gingivalis
Prevotella melani­nog­enica
Bile Tolerance (20% Bile)
+
-
-
Brick Red Fluore­scence (UV light)
-
+
+
Brown/­Black Colonies on CDC
-
+
+
Vancomycin
R
S
R
Penicillin
R
S
R
Kanamycin
R
R
R
Lipase
-
-
-
Esculin
+
-
-

Gram Positive Bacilli

Catalase Positive
Cutiba­cterium (former Propio­nib­act­erium)
- Moderate Growth Rate
- Diphth­eroid Gram Stain
Catalase Negative
Actino­myces spp.
- Slow growth rate
- Filame­ntous, branching GPB
Lactob­acillus
- Rapid Growth Rate{{nl}- Looping, chaining GPB
- Resistant to Vancomycin
Clostr­idium spp.
- Clostr­idium perfri­ngens
- Lecith­inase Positive
- cloudy around colony
- Double zone beta hemolysis
- Lipase negative
- Boxcar shape
- Gangrene
- Clostr­idium sporogenes
- Common in soil
- Comes from stool samples
- Lipase Positive
- Smells like bad dirt
- Clostr­idium difficile
- Should not be grown
- Do toxin testing and PCR
- Test of cure
- PCR can pick up dead DNA so it can be false positive
- If pt has firm stool, no longer transm­issable
- Clostr­idium septicum
- Gangrene
- Spreading colony formation
- Subter­minal Spores
- Clostr­idium tetani
- Cause of tetanus
- Club like
- Tennis racket like
- Clostr­idium tertium
- Aerotolerant
- Can grow in O2

Gram Positive Cocci

Peptos­tre­pto­coccus
Catalase Negative
Staphy­loc­occus saccha­rol­yticus
Catalase Variable

Gram Negative Cocci

Veillo­nella spp.
Sometimes in blood cultures from endoca­rditis
 

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  • Published: 11th August, 2019

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