Smooth Muscle Features
Non-striated. Uninuclear. Spindle-shaped cells (dia = 2-10 μm, l = 50-400 μm). Divide lifelong. Thick filaments = myosin. Thin filaments = actin, anchored to the plasma membrane or dense bodies. Filaments are not organized into myofibrils, no sarcomeres. No troponin, calmodulin instead! Tropomyosin present. Contract by sliding-filament mechanism. No T-tubules. Sarcoplasmic reticulum present. Activation of Smooth Muscle Contraction by Ca+2 |
Single Unit Smooth MuscleCells respond to stimuli as a single unit due to connections via gap junctions = functional syncytium. Capable of generating pacemaker activity. Spontaneous AP from pacemaker SMC can propogate to non-pacemaker SMC due to gap junctions. Experience pacemaker potential and slow-wave potential. Ex- GI tract walls, reproductive tract walls, urinary tract walls, walls of small blood vessels. Multi Unit Smooth MuscleSMCs that are activated by neuronal input. Cells respond to stimuli independently and contain few gap junctions. Ex- Walls of large blood vessels, large airways to lungs, muscles of the eye that adjust the lens, iris of the eye, base of hair follicles. Pacemaker vs. Slow-Wave Potential |
Excitation/Contraction Coupling in SMCs
Ca2+ entry triggers internal release of Ca2+ from SR. Ca2+ binds to calmodulin in cytosol. Ca2+-calmodulin complex activates light chain myosin kinase (phosphorylates light chain of myosin). Phosphorylated myosin light chain binds to actin = activated cross-bridges. Removal of Ca2+ desphosphorylates myosin, dissociating it from actin. Gap junctions allow rapid spread of excitation between connected cells. Contraction strength is directly proportional to cytosolic [Ca2+]. |
Cheatography
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NPB 101 Smooth Muscles Cheat Sheet (DRAFT) by schat
NPB 101 Smooth Muscles.
This is a draft cheat sheet. It is a work in progress and is not finished yet.