This is a draft cheat sheet. It is a work in progress and is not finished yet.
Decalcification
Process of removing lime salts or calcium salts from bone |
Makes bone flexible and easy to cut |
Adjusts the hard substance of bones to the softness of paraffin embedding medium. |
Specimen: Bone, Teeth. Calcified Tumors, Calcified Heart Valves |
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Purpose of Decalcification
Facilitate cutting of bone |
Remove bone dust and cellular debris |
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Factors Affecting Decalcification
Size and thickness of specimen |
Dense Bone: 2-5 cm
Soft Bones: 4-6 cm |
Compactness and Density of Bone |
Ideal time: 24-48 hrs
Very Dense: =>14 days |
Concentration and Amount of Fluid |
20:1 |
Temperature |
Room Temperature |
Agitation |
Stirring from time to time |
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