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Clinical Chemistry Review Chapter 6 & 7 Cheat Sheet (DRAFT) by

This is a draft cheat sheet. It is a work in progress and is not finished yet.


Group of techniques used to separate complex mixtures on basis of different physical intera­ctions between a stationary and mobile phase
Basic components
- mobile phase (gas or liquid)
- stationary phase (solid or liquid)
- column holding stationary phase
- separated components (eluate)
- complex mixture (sample)
- Planar
- Paper
- Gas (GC)- can be mobile or stationary (polymer silica)
- Liquid (LC) – mobile phase – high pressure and connected to mass spec
Modes of Separation
- Adsorption – liquid­-Solid (hydro­phobic and Hydrop­hilic bonds)
- Partition – Liquid­-Liquid
- Steric exclusion – Variation of liquid­-Solid
- Ion-ex­change chroma­tog­raphy – Magnitude and charge (separ­ation of amino acids)
- Affinity


Thin-layer chroma­tog­raphy (TLC)
- A variant of column chroma­tog­raphy
- Thin layer of sorbent is uniformly coated on glass/­plastic plate.
- Samples are applied as spots near edge of plate.
- One edge of plate is placed in solvent.
- Solvent migrates up thin layer by capillary action.
- After solvent reaches predet­ermined height, plate is removed and dried.


- Uses pressure for fast separa­tions, controlled temper­ature, in-line detectors, and gradient elution techniques
- Compon­ents: pumps, columns, sample injectors, detectors, recorders