This is a draft cheat sheet. It is a work in progress and is not finished yet.
Group of techniques used to separate complex mixtures on basis of different physical interactions between a stationary and mobile phase
- mobile phase (gas or liquid)
- stationary phase (solid or liquid)
- column holding stationary phase
- separated components (eluate)
- complex mixture (sample)
- Gas (GC)- can be mobile or stationary (polymer silica)
- Liquid (LC) – mobile phase – high pressure and connected to mass spec
Modes of Separation
- Adsorption – liquid-Solid (hydrophobic and Hydrophilic bonds)
- Partition – Liquid-Liquid
- Steric exclusion – Variation of liquid-Solid
- Ion-exchange chromatography – Magnitude and charge (separation of amino acids)
Thin-layer chromatography (TLC)
- A variant of column chromatography
- Thin layer of sorbent is uniformly coated on glass/plastic plate.
- Samples are applied as spots near edge of plate.
- One edge of plate is placed in solvent.
- Solvent migrates up thin layer by capillary action.
- After solvent reaches predetermined height, plate is removed and dried.
HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC)
- Uses pressure for fast separations, controlled temperature, in-line detectors, and gradient elution techniques
- Components: pumps, columns, sample injectors, detectors, recorders