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Cheatography

Tumor Markers Cheat Sheet by

Immunohistochemistry

Epithelial Tumor Markers

Keratin
Highly Sensitive Marker for epithelial Cells
Epithelial Membrane Antigen (EMA)
High molecular weight protein that is helpful for determ­ining the site of tumor
Carcin­oem­bryonic Antigen (CEA)
Oncofetal antigen in carcinomas of GIT, pancreas, lung, breast, ovary, uterus, and cervix
Thyroid Transc­ription Factor-1 (TFF-1)
Useful for distin­gui­shing lung adenoc­arc­inomas from mesoth­eliomas from the mesoth­elium
Prostate Specific Antigen (PSA)
Extremely useful in diagnosis of prostatic adenoc­arc­inoma

Interm­ediate Filament Markers

Actin
Tumors of smooth, skeletal, and cardiac muscle
Vimentin
Melanoma
Schwannomas
Desmin
Leiomymoa
Rhabdomyosarcoma
Glial Fibrillary Acidic Protein (GFAP)
Astrocytoma
Ependomyomas
Oligodendrogliomas
Medulloblastoma
If negative: Non-CNS Tumors
Meningiomas
Metastatic Carcinomas
Lymphomas
Neurof­ila­ments
Neurof­ila­ments (NF)
Neuroblastoma
Ganglioneuromas
Chemodectomas
Pheochromocytomas
Note: Show neuroe­ndo­crine differ­ent­iation
S100 Protein

Mesenc­hymal Tumor Markers

Myogenic Tumors
Tumors of skeletal muscle origin (Positive for muscle­-sp­ecific actin and desmin)
Fibroh­ist­ocytic Tumor
(CD68 and FAM 56)
Malignant fibroh­ist­ocytic sarcomas
Note: Combined with alpha1­-An­tit­rypsin and alpha1­-an­tic­hym­otr­ypsin
Vascular Tumor
Factor VII-re­lated Antigen, CD31, Ulex Eurpaeus 1 (UEA)

Cancer­-As­soc­iated Genes

p53
Tumor suppressor gene
C-erb-­2/c­-my­c/ras
Cellular oncogene found to be activated in breast cancer
 

Epithelial Membrane Antigen (EMA)

EMA+
Adenoc­arc­inoma of Breast
Adenocarcinoma of Lungs
Adenocarcinoma of Kidneys
EMA-
Non-ep­­it­h­elial tumors
Sarcomas Lymphomas
Melanomas
EMA Non-re­active
Hepato­­ce­l­lular Carcinoma
Adrenal Carcinoma
Embryonal Carcinoma

Carcin­­oe­m­b­ryonic Antigen (CEA)

CEA+
Adenoc­arc­inoma
CEA-
Mesoth­­elioma
CEA Non-re­active
Prostate Carcinoma
Thyroid Carinoma
Renal Carcinoma

Prostate Specific Antigen (PSA)

PSA+
Prostatic Adenocarcinoma
Pancreatic Tumor
Salivary Gland Tumor

Germ Cell Tumor Markers

Human Chorionic Gonado­tropin (HCG)
Marker for Chorio­-ca­rcinoma
Alpha-­fet­opr­otein (AFP)
Embryonal Carcinomas
Treatomas
Hepatocelullar Carcinomas
Placen­ta-like Alkaline Phosph­atase (PLAP)
Most embryonal CA
Choriocarcinoma
Endodermal Sinus Tumors
Majority of seminomas

Lymphomas

Leukocyte Common Antigen (LCA)
CD45
T-cells
CD3, CD4, CD8
B-cells
CD19, CD20, CD23
Reed-S­ter­nberg Cells
CD15, CD30
Immuno­glo­bulin Light and Heavy Chains
 

CK7+, CK20-

CK7+, CK20-
Lung Carcinoma
Breast Carcinoma
Uterus Carcinoma
Ovaries Carcinoma
Serous Tumors
CK7-, CK20+
Colon Carcinoma
Stomach Carcinoma
CK7+, CK20+
Transi­­tional Cell Carcinoma (Bladder)
Mucinous Ovarian Tumor
CK7-, CK20-
Renal Cell Carcinoma
Hepato­cel­lular Carcinoma
Prostatic Adenoc­arc­inoma
Thyroid Carcinoma
Squamous Cell Carcinoma

Thyroid Transc­­ri­ption Factor-1 (TFF-1)

TFF-1+
Thyroid, Lung, Neuroe­ndo­crine Tumors
Medullary Carcinoma
Carcinoid Tumor
Small Cell Tumor of Lungs

Neuroe­ndo­crine Markers

Neuron­-Sp­ecific Enolase
Provides strong evidence of neural or neuroe­ndo­crine differ­ent­iation
Chromo­granin
Neuroe­ndo­crine differentiation
Granular immuno­rea­ctivity
K+,C-
Neuroendocrine Carcinoma
K-,C+
Paraganglioma
Synapt­ophysin
Associated with presyn­aptic vesicles of neurons

Melanomas

Melanosome (HMB-45)
Detection of primary and secondary melanomas
Highly sensitive and specific
Melan-A (MART-1)
Encodes melano­ma-­spe­cific antigen in normal pigmented cells of skin, retina, and certain adreno­cor­tical tumors

Cell Prolif­eration Markers

Ki-67 (MIB-1) and Prolif­erating Cell Nuclear Antigen (PCNA)
Assess the prolif­eration of tumor cells
 

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