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Cheatography

Microscopy Techniques Cheat Sheet (DRAFT) by

Med Micro Lecture 1

This is a draft cheat sheet. It is a work in progress and is not finished yet.

Microscopy

Magnif­ication: ability to enlarge objects
Resolution: capacity to distin­gui­sh/­sep­arate 2 adjacent objects. Depends on wavelength being used

Light (Optical) Microscopy

Bright­-Field
Phase-­Con­trast
Dark-Field
Fluore­scence
   

Electron Microscopy

-Forms an image using a beam of electrons that can be made to travel in wavelike patterns when accele­rated to high speeds
-Electron waves are 100,000 times shorter than the waves of visible light and thus provide tremendous resolution