Short Reads Sequencing Technologies Cheat Sheet (DRAFT) by rekins

Illumina	MGI	Element Bio
Bridge amplif­ication	Rolling Circle Amplif­ication (RCA)	Rolling Circle Amplif­ication (RCA)
PCR	NO error propag­ation by PCR	NO error propag­ation by PCR
Clusters	DNA Nano Balls (DNB)	Polonies

Illumina	MGI	Element Bio
Non Patterned FC	X	Non Patterned FC
Patterned FC	Patterned FC	X
up to 600 cycles (MiSeq)	up to 600 cycles	up to 600 cycles
4 lanes (NovaSeq S4)	4 lanes	2 lanes
up to 12B reads (NovaSeq S4)	1500M-1800M reads	250M-500M-1B reads

Illumina	MGI	Element Bio
Sequencing by synthesis	cPAS sequencing	Sequencing by Avidity
Fluorescent Nucleo­tides	Fluorescent Nucleo­tides	Avidite

Illumina	MGI	Element Bio
Q30	Q35	Q40
Index Hopping (2%)	Index Hopping (negli­gible)	Avidite (negli­gible)
Duplication (+++)		Duplication (low)
AT/GC bias		low AT/GC bias

Illumina	MGI	Element Bio
		robust to low diversity libraries
insert size (200–800 bp)	insert size (300-500 bp)	insert size (175-1000 bp)