This is a draft cheat sheet. It is a work in progress and is not finished yet.
Fluorescent Dyes
Fluorophore |
Absorbs |
Emits |
Marks |
DAPI |
UV (365 nm) |
Blue (> 420 nm) |
nucleic acids |
Texas-Red Phalloidin |
Yellow-Green (552 nm) |
Red (570 nm) |
actin |
BODIPY FL |
Blue (495 nm) |
Green (525 nm) |
tubulin |
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Plants
Bryophyte |
moss |
Seedless vascular |
fern |
Gymnosperm |
conifers |
Angiosperm |
majority of land plants - flowers |
Dicot vs Monocot
Dicot |
Monocot |
2 cotyledons |
1 cotyledon |
netlike leaf |
parallel, straight leaf |
4-5 flowers |
multiple of 3 flowers |
ring stem |
scattered bundles in stem |
x in root |
ring in root |
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PCR
Steps? Denature, Anneal, Extend
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