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\author{UmeshJagtap}
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  /Title (study-of-peroxisomes-catalase-activity.pdf)
  /Creator (Cheatography)
  /Author (UmeshJagtap)
  /Subject (Study of Peroxisomes (Catalase activity) Cheat Sheet)
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    \vspace{-2pt}\large{\bf{\textcolor{DarkBackground}{\textrm{Study of Peroxisomes (Catalase activity) Cheat Sheet}}}} \\
    \normalsize{by \textcolor{DarkBackground}{UmeshJagtap} via \textcolor{DarkBackground}{\uline{cheatography.com/186232/cs/41874/}}}
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  \mymulticolumn{2}{p{5.377cm}}{\bf\textcolor{white}{Cheatographer}}  \\
  \vspace{-2pt}UmeshJagtap \\
  \uline{cheatography.com/umeshjagtap} \\
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  \mymulticolumn{1}{p{5.377cm}}{\bf\textcolor{white}{Cheat Sheet}}  \\
   \vspace{-2pt}Published 6th January, 2024.\\
   Updated 28th December, 2023.\\
   Page {\thepage} of \pageref{LastPage}.
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  Measure your website readability!\\
  www.readability-score.com
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\begin{multicols*}{2}

\begin{tabularx}{8.4cm}{X}
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\mymulticolumn{1}{x{8.4cm}}{\bf\textcolor{white}{Objective}}  \tn
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\mymulticolumn{1}{x{8.4cm}}{To investigate the activity of catalase, an enzyme contained within peroxisomes, by observing its ability to break down hydrogen peroxide (H2O2)% Row Count 3 (+ 3)
} \tn 
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\begin{tabularx}{8.4cm}{X}
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\mymulticolumn{1}{x{8.4cm}}{\bf\textcolor{white}{Background}}  \tn
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\mymulticolumn{1}{x{8.4cm}}{Peroxisomes are small, membrane-bound organelles found in virtually all eukaryotic cells. They are involved in various metabolic processes, including the breakdown of very long chain fatty acids through beta-oxidation. One of their key roles is in the detoxification of hydrogen peroxide, a byproduct of cellular metabolism, into water and oxygen. This reaction is catalyzed by the enzyme catalase, which is abundantly present in peroxisomes.% Row Count 9 (+ 9)
} \tn 
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\mymulticolumn{1}{x{8.4cm}}{\bf\textcolor{white}{Materials}}  \tn
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\mymulticolumn{1}{x{8.4cm}}{\{\{fa-square-o\}\} Fresh potato (rich source of catalase) \newline % Row Count 2 (+ 2)
\{\{fa-square-o\}\} 3\% Hydrogen peroxide solution \newline % Row Count 3 (+ 1)
\{\{fa-square-o\}\} Test tubes or small beakers \newline % Row Count 4 (+ 1)
\{\{fa-square-o\}\} Tweezers or forceps \newline % Row Count 5 (+ 1)
\{\{fa-square-o\}\} Knife or scalpel \newline % Row Count 6 (+ 1)
\{\{fa-square-o\}\} Cork Borer  \newline % Row Count 7 (+ 1)
\{\{fa-square-o\}\} Graduated cylinder or pipette \newline % Row Count 8 (+ 1)
\{\{fa-square-o\}\} Stopwatch or timer \newline % Row Count 9 (+ 1)
\{\{fa-square-o\}\} Safety goggles and gloves% Row Count 10 (+ 1)
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\mymulticolumn{2}{x{8.4cm}}{\bf\textcolor{white}{Procedure}}  \tn
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{\bf{Preparation of Materials:}} & 1. Clean and set up your workspace with all the materials listed above. \{\{nl\}\} 2. Label your test tubes or beakers for identification. \tn 
% Row Count 7 (+ 7)
% Row 1
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{\bf{Preparation of Potato Samples:}} & 3. Wash the potato and dry it. \{\{nl\}\} 4. On the chopping board, use the cork borer, scalpel or knife to cut the potato into small, equal-sized pieces (approximately 1 cm$^{\textrm{3}}$). \tn 
% Row Count 16 (+ 9)
% Row 2
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{\bf{Preparation of Hydrogen Peroxide Solutions:}} & 5. Using the graduated cylinder or pipette, measure and pour 10 ml of 3\% hydrogen peroxide into each test tube or beaker. \tn 
% Row Count 23 (+ 7)
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{\bf{Conducting the Experiment:}} & 6. Add a single piece of potato to the first test tube containing hydrogen peroxide. \{\{nl\}\} 7. As soon as the potato is added, start the stopwatch. \{\{nl\}\} 8. Observe the reaction between the potato and hydrogen peroxide for 1 minute. \{\{nl\}\} 9. Look for bubbling or fizzing, indicating the release of oxygen gas. \{\{nl\}\} 10. Record your observations in detail, focusing on the intensity and duration of the bubbling. \tn 
% Row Count 44 (+ 21)
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{\bf{Repeat the Experiment:}} & 11. For accuracy and consistency, repeat the experiment with fresh hydrogen peroxide and a new potato piece in the remaining test tubes or beakers \tn 
% Row Count 8 (+ 8)
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{\bf{Recording Data:}} & 12. In your lab notebook, create a table to record your observations for each test tube. Note the size of the potato piece, the amount of hydrogen peroxide used, the intensity of the reaction, and any other notable observations in observation table. \tn 
% Row Count 21 (+ 13)
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{\bf{Clean up}} & Dispose of the potato pieces and hydrogen peroxide solution in the waste container. Clean and sterilize the test tubes, beakers, and other equipment used. \tn 
% Row Count 29 (+ 8)
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\mymulticolumn{1}{x{8.4cm}}{\bf\textcolor{white}{Conclusion}}  \tn
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\mymulticolumn{1}{x{8.4cm}}{This experiment should demonstrate the enzymatic activity of catalase present in potatoes, as evidenced by the breakdown of hydrogen peroxide into water and oxygen, observable through the production of bubbles. The intensity and duration of bubbling provide insights into the enzyme's activity under the given conditions.% Row Count 7 (+ 7)
} \tn 
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% That's all folks
\end{multicols*}

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