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\author{UmeshJagtap}
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  /Title (micrometry-technique.pdf)
  /Creator (Cheatography)
  /Author (UmeshJagtap)
  /Subject (Micrometry Technique Cheat Sheet)
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\begin{multicols}{3}
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    {\parbox{\dimexpr\textwidth-2\fboxsep\relax}{\noindent
        \hspace*{-6pt}\includegraphics[width=5.8cm]{/web/www.cheatography.com/public/images/cheatography_logo.pdf}}
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\begin{tabulary}{11cm}{L}
    \vspace{-2pt}\large{\bf{\textcolor{DarkBackground}{\textrm{Micrometry Technique Cheat Sheet}}}} \\
    \normalsize{by \textcolor{DarkBackground}{UmeshJagtap} via \textcolor{DarkBackground}{\uline{cheatography.com/186232/cs/39996/}}}
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  \mymulticolumn{2}{p{5.377cm}}{\bf\textcolor{white}{Cheatographer}}  \\
  \vspace{-2pt}UmeshJagtap \\
  \uline{cheatography.com/umeshjagtap} \\
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  \mymulticolumn{1}{p{5.377cm}}{\bf\textcolor{white}{Cheat Sheet}}  \\
   \vspace{-2pt}Published 25th August, 2023.\\
   Updated 25th August, 2023.\\
   Page {\thepage} of \pageref{LastPage}.
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  Measure your website readability!\\
  www.readability-score.com
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\begin{multicols*}{2}

\begin{tabularx}{8.4cm}{X}
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\mymulticolumn{1}{x{8.4cm}}{\bf\textcolor{white}{AIM}}  \tn
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\mymulticolumn{1}{x{8.4cm}}{Measurements using the Ocular Micrometer (Micrometry Technique).% Row Count 2 (+ 2)
} \tn 
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\begin{tabularx}{8.4cm}{X}
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\mymulticolumn{1}{x{8.4cm}}{\bf\textcolor{white}{OBJECTIVES}}  \tn
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\mymulticolumn{1}{x{8.4cm}}{1. Calibrate the Ocular Micrometer Scale. \{\{nl\}\}2. Measure samples using the Ocular Micrometer Scale.} \tn 
% Row Count 3 (+ 3)
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\begin{tabularx}{8.4cm}{X}
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\mymulticolumn{1}{x{8.4cm}}{\bf\textcolor{white}{INTRODUCTION}}  \tn
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\mymulticolumn{1}{x{8.4cm}}{Micrometer scales within the ocular of a microscope are employed to determine sample sizes. These specialized oculars possess a transparent scale graticule superimposed onto the observed image. By replacing the stage micrometer with the sample slide, you can measure cell sizes. Micrometry is a crucial technique in biology, especially in microscopy, using ocular and stage micrometers to measure biological structures.% Row Count 9 (+ 9)
} \tn 
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\mymulticolumn{2}{x{8.4cm}}{\bf\textcolor{white}{1. OCULAR MICROMETER}}  \tn
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{\bf{Description:}} & Transparent ruler-like device without units, mounted on eyepiece. \tn 
% Row Count 3 (+ 3)
% Row 1
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{\bf{Design:}} & Glass disc featuring 10 mm scale divided into 100 sub-divisions. \tn 
% Row Count 6 (+ 3)
% Row 2
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{\bf{Visibility:}} & Scale visible through microscope eyepiece. \tn 
% Row Count 8 (+ 2)
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\mymulticolumn{2}{x{8.4cm}}{\bf\textcolor{white}{2. STAGE MICROMETER}}  \tn
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{\bf{Placement:}} & Positioned on microscope stage. \tn 
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{\bf{Construction:}} & Slide with 2 mm scale divided into 0.01 mm (10 µm) sub-divisions. \tn 
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{\bf{Function:}} & Used to calculate ocular micrometer divisions at specific magnifications. \tn 
% Row Count 8 (+ 3)
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\begin{tabularx}{8.4cm}{X}
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\mymulticolumn{1}{x{8.4cm}}{\bf\textcolor{white}{REQUIREMENTS}}  \tn
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\mymulticolumn{1}{x{8.4cm}}{\{\{fa-square-o\}\}Compound light microscope with bjectives (4X, 10X, 20X, 40X),  \newline % Row Count 2 (+ 2)
\{\{fa-square-o\}\}Ocular micrometer scale \newline % Row Count 3 (+ 1)
\{\{fa-square-o\}\}Stage micrometer (0.01 mm scale). \newline % Row Count 4 (+ 1)
\{\{fa-square-o\}\}Microscope Glass slide \newline % Row Count 5 (+ 1)
\{\{fa-square-o\}\}Coverslip% Row Count 6 (+ 1)
} \tn 
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\begin{tabularx}{8.4cm}{X}
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\mymulticolumn{1}{x{8.4cm}}{\bf\textcolor{white}{PROTOCOL}}  \tn
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\mymulticolumn{1}{x{8.4cm}}{{\bf{Part I: Calibration of Ocular Micrometer}} \newline % Row Count 1 (+ 1)
1. Place the ocular micrometer in the microscope eyepiece. \newline % Row Count 3 (+ 2)
2. Adjust the lighting for optimal Kohler illumination. \newline % Row Count 5 (+ 2)
3. Focus on the stage micrometer using the lowest magnification. \newline % Row Count 7 (+ 2)
4. Rotate to the objective designated for calibration. \newline % Row Count 9 (+ 2)
5.Adjust the focus of the ocular micrometer to visualize both scales clearly. \newline % Row Count 11 (+ 2)
6. Align the stage and ocular scales with a slight offset for ease of reading. \newline % Row Count 13 (+ 2)
7. Choose two positions on each scale, preferably on opposite sides. \newline % Row Count 15 (+ 2)
8. Count the divisions on the stage scale (ssd) between the chosen positions. \newline % Row Count 17 (+ 2)
9. Count the divisions on the ocular scale (osd), which equals ssd. \newline % Row Count 19 (+ 2)
10. Record the number of ocular spaces (y) and stage spaces (x). \newline % Row Count 21 (+ 2)
11.Calculate the calibration factor: 1 ocular space = (x/y) × 10 µm. \newline % Row Count 23 (+ 2)
12.Repeat the process for the second assigned objective.% Row Count 25 (+ 2)
} \tn 
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\mymulticolumn{1}{x{8.4cm}}{Microscope Field of View Showing Ocular Micrometer and Stage Micrometer Superimposed each other.}  \tn 
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\mymulticolumn{1}{x{8.4cm}}{\bf\textcolor{white}{Part II: Protocol for Using Micrometers}}  \tn
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\mymulticolumn{1}{x{8.4cm}}{1. Place biological sample slide (e.g. Pollen Grain), focus on area. \newline % Row Count 2 (+ 2)
2. Locate ocular micrometer through eyepiece. \newline % Row Count 3 (+ 1)
3. Align structure with ocular micrometer. \newline % Row Count 4 (+ 1)
4. Count ocular divisions needed to span the structure. \newline % Row Count 6 (+ 2)
5. Repeat for other objectives and other biological specimens. \newline % Row Count 8 (+ 2)
6. Record divisions for each measurement. \newline % Row Count 9 (+ 1)
7. Calculate structure size (e.g. pollen grain): Ocular divisions × calibration factor. \newline % Row Count 11 (+ 2)
8. This protocol accurately measures biological structures.% Row Count 13 (+ 2)
} \tn 
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\mymulticolumn{1}{x{8.4cm}}{Plant Samples} \tn 
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\mymulticolumn{1}{x{8.4cm}}{Size of Pollen Grain under 10X = \_\_\_\_\_\_\_\_\_ µm} \tn 
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\mymulticolumn{1}{x{8.4cm}}{Size of Pollen Grain under 40X = \_\_\_\_\_\_\_\_\_ µm} \tn 
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\mymulticolumn{1}{x{8.4cm}}{\bf\textcolor{white}{CONCLUSION}}  \tn
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\mymulticolumn{1}{x{8.4cm}}{In conclusion, micrometer scales within microscope oculars are pivotal for accurate sample size determination in biology. By replacing the stage micrometer with a sample slide, cell sizes can be measured% Row Count 5 (+ 5)
} \tn 
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% That's all folks
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