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% Document Info
\author{Sourav Pan (Sourav355)}
\pdfinfo{
  /Title (flow-cytometry-cheat-sheet.pdf)
  /Creator (Cheatography)
  /Author (Sourav Pan (Sourav355))
  /Subject (Flow Cytometry Cheat Sheet)
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        \hspace*{-6pt}\includegraphics[width=5.8cm]{/web/www.cheatography.com/public/images/cheatography_logo.pdf}}
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    \vspace{-2pt}\large{\bf{\textcolor{DarkBackground}{\textrm{Flow Cytometry Cheat Sheet}}}} \\
    \normalsize{by \textcolor{DarkBackground}{Sourav Pan (Sourav355)} via \textcolor{DarkBackground}{\uline{cheatography.com/186860/cs/39088/}}}
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  \mymulticolumn{2}{p{5.377cm}}{\bf\textcolor{white}{Cheatographer}}  \\
  \vspace{-2pt}Sourav Pan (Sourav355) \\
  \uline{cheatography.com/sourav355} \\
        \uline{\seqsplit{biologynotesonline}.com/}
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  \mymulticolumn{1}{p{5.377cm}}{\bf\textcolor{white}{Cheat Sheet}}  \\
   \vspace{-2pt}Published 4th June, 2023.\\
   Updated 4th June, 2023.\\
   Page {\thepage} of \pageref{LastPage}.
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  Measure your website readability!\\
  www.readability-score.com
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\begin{document}
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\begin{multicols*}{2}

\begin{tabularx}{8.4cm}{X}
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\mymulticolumn{1}{x{8.4cm}}{\bf\textcolor{white}{What is Flow Cytometry?}}  \tn
\SetRowColor{white}
\mymulticolumn{1}{x{8.4cm}}{Flow cytometry is a technique used to analyze and measure characteristics of cells or particles in a fluid sample. \newline % Row Count 3 (+ 3)
It provides quantitative data about cell populations based on their physical and chemical properties.% Row Count 6 (+ 3)
} \tn 
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\par\addvspace{1.3em}

\begin{tabularx}{8.4cm}{x{2.48 cm} x{5.52 cm} }
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\mymulticolumn{2}{x{8.4cm}}{\bf\textcolor{white}{Basic Components of a Flow Cytometer}}  \tn
% Row 0
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Fluidics System & Controls the flow of the sample through the instrument. \tn 
% Row Count 3 (+ 3)
% Row 1
\SetRowColor{white}
Optics System & ncludes lasers, detectors, and filters for detecting and measuring light signals. \tn 
% Row Count 6 (+ 3)
% Row 2
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Electronics System & Converts optical signals into electronic signals for data analysis. \tn 
% Row Count 9 (+ 3)
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\begin{tabularx}{8.4cm}{X}
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\mymulticolumn{1}{x{8.4cm}}{\bf\textcolor{white}{Data Acquisition and Analysis}}  \tn
% Row 0
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\mymulticolumn{1}{x{8.4cm}}{Forward Scatter (FSC): Measures the size of particles/cells.} \tn 
% Row Count 2 (+ 2)
% Row 1
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\mymulticolumn{1}{x{8.4cm}}{Side Scatter (SSC): Reflects the internal complexity or granularity of particles/cells.} \tn 
% Row Count 4 (+ 2)
% Row 2
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\mymulticolumn{1}{x{8.4cm}}{Fluorescence Detectors: Capture emitted light at specific wavelengths from labeled cells.} \tn 
% Row Count 6 (+ 2)
% Row 3
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\mymulticolumn{1}{x{8.4cm}}{Compensation: Corrects spectral overlap between fluorochromes to obtain accurate fluorescence measurements.} \tn 
% Row Count 9 (+ 3)
% Row 4
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\mymulticolumn{1}{x{8.4cm}}{Gating: Defines specific populations of interest within the sample based on fluorescence intensity and scatter characteristics.} \tn 
% Row Count 12 (+ 3)
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\begin{tabularx}{8.4cm}{X}
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\mymulticolumn{1}{x{8.4cm}}{\bf\textcolor{white}{Considerations and Tips:}}  \tn
% Row 0
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\mymulticolumn{1}{x{8.4cm}}{Choose appropriate fluorochromes and antibodies for specific targets.} \tn 
% Row Count 2 (+ 2)
% Row 1
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\mymulticolumn{1}{x{8.4cm}}{Use appropriate controls (positive, negative, compensation) to ensure accurate results.} \tn 
% Row Count 4 (+ 2)
% Row 2
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\mymulticolumn{1}{x{8.4cm}}{Maintain instrument performance through regular cleaning, calibration, and quality control.} \tn 
% Row Count 6 (+ 2)
% Row 3
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\mymulticolumn{1}{x{8.4cm}}{Optimize staining protocols and instrument settings for best results.} \tn 
% Row Count 8 (+ 2)
% Row 4
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\mymulticolumn{1}{x{8.4cm}}{Data analysis software skills are crucial for extracting meaningful information.} \tn 
% Row Count 10 (+ 2)
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\par\addvspace{1.3em}

\begin{tabularx}{8.4cm}{X}
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\mymulticolumn{1}{x{8.4cm}}{\bf\textcolor{white}{Staining and Sample Preparation}}  \tn
% Row 0
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\mymulticolumn{1}{x{8.4cm}}{Fluorescent Labels: Use fluorescent dyes or antibodies to label specific cell components or markers of interest.} \tn 
% Row Count 3 (+ 3)
% Row 1
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\mymulticolumn{1}{x{8.4cm}}{Fixation and Permeabilization: Helps preserve cell structure and allows the entry of antibodies into the cell.} \tn 
% Row Count 6 (+ 3)
% Row 2
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\mymulticolumn{1}{x{8.4cm}}{Compensation Controls: Used to correct for spectral overlap between different fluorochromes.} \tn 
% Row Count 8 (+ 2)
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\par\addvspace{1.3em}

\begin{tabularx}{8.4cm}{X}
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\mymulticolumn{1}{x{8.4cm}}{\bf\textcolor{white}{Applications of Flow Cytometry}}  \tn
% Row 0
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\mymulticolumn{1}{x{8.4cm}}{Immunophenotyping: Identifying and characterizing different cell types in a mixed population.} \tn 
% Row Count 2 (+ 2)
% Row 1
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\mymulticolumn{1}{x{8.4cm}}{Cell Cycle Analysis: Determining the distribution of cells in different phases of the cell cycle.} \tn 
% Row Count 4 (+ 2)
% Row 2
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\mymulticolumn{1}{x{8.4cm}}{Apoptosis Detection: Assessing cell death and apoptosis using markers like Annexin V and propidium iodide.} \tn 
% Row Count 7 (+ 3)
% Row 3
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\mymulticolumn{1}{x{8.4cm}}{Intracellular Protein Analysis: Analyzing protein expression within cells using antibodies and permeabilization methods.} \tn 
% Row Count 10 (+ 3)
% Row 4
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\mymulticolumn{1}{x{8.4cm}}{Cell Sorting: Separating specific cell populations based on defined criteria using high-speed droplet-based sorting.} \tn 
% Row Count 13 (+ 3)
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\begin{tabularx}{8.4cm}{X}
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\mymulticolumn{1}{x{8.4cm}}{\bf\textcolor{white}{Read More About Flow Cytometry}}  \tn
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\mymulticolumn{1}{x{8.4cm}}{\{\{link="https://microbiologynote.com/flow-cytometry/"\}\}Flow Cytometry – Types, Purpose, Reagents, Examples, Application \{\{/link\}\}} \tn 
% Row Count 3 (+ 3)
% Row 1
\SetRowColor{white}
\mymulticolumn{1}{x{8.4cm}}{\{\{link="https://microbiologynote.com/flow-cytometry-principle-process-uses/"\}\}Flow cytometry Principle, Process, Uses  \{\{/link\}\}} \tn 
% Row Count 6 (+ 3)
% Row 2
\SetRowColor{LightBackground}
\mymulticolumn{1}{x{8.4cm}}{\{\{link="https://microbiologynote.com/flow-cytometry-laboratory-for-cell-viability-staining-using-propidium-iodide/"\}\}Flow Cytometry Laboratory for cell viability staining using propidium iodide.  \{\{/link\}\}} \tn 
% Row Count 11 (+ 5)
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\par\addvspace{1.3em}


% That's all folks
\end{multicols*}

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