\documentclass[10pt,a4paper]{article} % Packages \usepackage{fancyhdr} % For header and footer \usepackage{multicol} % Allows multicols in tables \usepackage{tabularx} % Intelligent column widths \usepackage{tabulary} % Used in header and footer \usepackage{hhline} % Border under tables \usepackage{graphicx} % For images \usepackage{xcolor} % For hex colours %\usepackage[utf8x]{inputenc} % For unicode character support \usepackage[T1]{fontenc} % Without this we get weird character replacements \usepackage{colortbl} % For coloured tables \usepackage{setspace} % For line height \usepackage{lastpage} % Needed for total page number \usepackage{seqsplit} % Splits long words. %\usepackage{opensans} % Can't make this work so far. Shame. Would be lovely. \usepackage[normalem]{ulem} % For underlining links % Most of the following are not required for the majority % of cheat sheets but are needed for some symbol support. \usepackage{amsmath} % Symbols \usepackage{MnSymbol} % Symbols \usepackage{wasysym} % Symbols %\usepackage[english,german,french,spanish,italian]{babel} % Languages % Document Info \author{Morghay123} \pdfinfo{ /Title (microscopy-cell-structure-staining.pdf) /Creator (Cheatography) /Author (Morghay123) /Subject (Microscopy, Cell Structure, Staining Cheat Sheet) } % Lengths and widths \addtolength{\textwidth}{6cm} \addtolength{\textheight}{-1cm} \addtolength{\hoffset}{-3cm} \addtolength{\voffset}{-2cm} \setlength{\tabcolsep}{0.2cm} % Space between columns \setlength{\headsep}{-12pt} % Reduce space between header and content \setlength{\headheight}{85pt} % If less, LaTeX automatically increases it \renewcommand{\footrulewidth}{0pt} % Remove footer line \renewcommand{\headrulewidth}{0pt} % Remove header line \renewcommand{\seqinsert}{\ifmmode\allowbreak\else\-\fi} % Hyphens in seqsplit % This two commands together give roughly % the right line height in the tables \renewcommand{\arraystretch}{1.3} \onehalfspacing % Commands \newcommand{\SetRowColor}[1]{\noalign{\gdef\RowColorName{#1}}\rowcolor{\RowColorName}} % Shortcut for row colour \newcommand{\mymulticolumn}[3]{\multicolumn{#1}{>{\columncolor{\RowColorName}}#2}{#3}} % For coloured multi-cols \newcolumntype{x}[1]{>{\raggedright}p{#1}} % New column types for ragged-right paragraph columns \newcommand{\tn}{\tabularnewline} % Required as custom column type in use % Font and Colours \definecolor{HeadBackground}{HTML}{333333} \definecolor{FootBackground}{HTML}{666666} \definecolor{TextColor}{HTML}{333333} \definecolor{DarkBackground}{HTML}{242CA3} \definecolor{LightBackground}{HTML}{F1F1F9} \renewcommand{\familydefault}{\sfdefault} \color{TextColor} % Header and Footer \pagestyle{fancy} \fancyhead{} % Set header to blank \fancyfoot{} % Set footer to blank \fancyhead[L]{ \noindent \begin{multicols}{3} \begin{tabulary}{5.8cm}{C} \SetRowColor{DarkBackground} \vspace{-7pt} {\parbox{\dimexpr\textwidth-2\fboxsep\relax}{\noindent \hspace*{-6pt}\includegraphics[width=5.8cm]{/web/www.cheatography.com/public/images/cheatography_logo.pdf}} } \end{tabulary} \columnbreak \begin{tabulary}{11cm}{L} \vspace{-2pt}\large{\bf{\textcolor{DarkBackground}{\textrm{Microscopy, Cell Structure, Staining Cheat Sheet}}}} \\ \normalsize{by \textcolor{DarkBackground}{Morghay123} via \textcolor{DarkBackground}{\uline{cheatography.com/53154/cs/17047/}}} \end{tabulary} \end{multicols}} \fancyfoot[L]{ \footnotesize \noindent \begin{multicols}{3} \begin{tabulary}{5.8cm}{LL} \SetRowColor{FootBackground} \mymulticolumn{2}{p{5.377cm}}{\bf\textcolor{white}{Cheatographer}} \\ \vspace{-2pt}Morghay123 \\ \uline{cheatography.com/morghay123} \\ \end{tabulary} \vfill \columnbreak \begin{tabulary}{5.8cm}{L} \SetRowColor{FootBackground} \mymulticolumn{1}{p{5.377cm}}{\bf\textcolor{white}{Cheat Sheet}} \\ \vspace{-2pt}Not Yet Published.\\ Updated 13th September, 2018.\\ Page {\thepage} of \pageref{LastPage}. \end{tabulary} \vfill \columnbreak \begin{tabulary}{5.8cm}{L} \SetRowColor{FootBackground} \mymulticolumn{1}{p{5.377cm}}{\bf\textcolor{white}{Sponsor}} \\ \SetRowColor{white} \vspace{-5pt} %\includegraphics[width=48px,height=48px]{dave.jpeg} Measure your website readability!\\ www.readability-score.com \end{tabulary} \end{multicols}} \begin{document} \raggedright \raggedcolumns % Set font size to small. Switch to any value % from this page to resize cheat sheet text: % www.emerson.emory.edu/services/latex/latex_169.html \footnotesize % Small font. \begin{multicols*}{2} \begin{tabularx}{8.4cm}{x{2.32 cm} x{5.68 cm} } \SetRowColor{DarkBackground} \mymulticolumn{2}{x{8.4cm}}{\bf\textcolor{white}{Bacterial Stains}} \tn % Row 0 \SetRowColor{LightBackground} {\bf{Simple Stain}} & -Single basic dye e.g. Methylene blue\{\{nl\}\}- All bacteria take the color of the dye \tn % Row Count 3 (+ 3) % Row 1 \SetRowColor{white} \mymulticolumn{2}{x{8.4cm}}{{\bf{Differential Stain}}} \tn % Row Count 4 (+ 1) % Row 2 \SetRowColor{LightBackground} Primary Stain & \_ First dye used in the staining process\{\{nl\}\}-Will initially stain all cells and then be removed from a subset \tn % Row Count 8 (+ 4) % Row 3 \SetRowColor{white} Mordant & Improves the ability of the primary stain to bind cells \tn % Row Count 10 (+ 2) % Row 4 \SetRowColor{LightBackground} \seqsplit{Decolorizer} & Removes the primary stain from a subset of cells \tn % Row Count 12 (+ 2) % Row 5 \SetRowColor{white} \seqsplit{Counterstain} & Second dye that stains decolorized cells \tn % Row Count 14 (+ 2) % Row 6 \SetRowColor{LightBackground} \mymulticolumn{2}{x{8.4cm}}{{\emph{Positive stain = purple color, negative charge}}} \tn % Row Count 15 (+ 1) % Row 7 \SetRowColor{white} \mymulticolumn{2}{x{8.4cm}}{{\emph{Negative stain = pink stain, negative charge}}} \tn % Row Count 16 (+ 1) \hhline{>{\arrayrulecolor{DarkBackground}}--} \end{tabularx} \par\addvspace{1.3em} \begin{tabularx}{8.4cm}{x{2.204 cm} x{2.736 cm} x{2.66 cm} } \SetRowColor{DarkBackground} \mymulticolumn{3}{x{8.4cm}}{\bf\textcolor{white}{Types of Microscopes}} \tn % Row 0 \SetRowColor{LightBackground} Light Microscope \seqsplit{(Bright-field)} & {\emph{Unstained}}\{\{nl\}\}Passes light directly through specimen\{\{nl\}\}unless cell is naturally pigmented or artificially stained, image has little contrast\{\{nl\}\}{\emph{Stained}}\{\{nl\}\}Staining with various dyes enhances contrast, but most staining procedures require that cells be fixed (preserved) & 1.Iris diaphragm\{\{nl\}\} 2.Condenser\{\{nl\}\} 3.Specimen\{\{nl\}\} 4.Objective lense\{\{nl\}\} 5.Eye\{\{nl\}\} \tn % Row Count 21 (+ 21) % Row 1 \SetRowColor{white} Dark-field Microscope & -Light is projected at an angle to the surface, causing any variations to deflect light up into the camera\{\{nl\}\}-Nothing is seen by the vision system if there are no aberrations on the surface\{\{nl\}\}-dark background makes the organism glow & 1.Light source\{\{nl\}\} 2.Dark Field Patch Stop\{\{nl\}\} 3.Condenser Lens\{\{nl\}\} 4.Sample\{\{nl\}\} 5.Direct Illumination Block\{\{nl\}\} 6.Objective Lens\{\{nl\}\} 7.Eyes\{\{nl\}\} \tn % Row Count 38 (+ 17) \end{tabularx} \par\addvspace{1.3em} \vfill \columnbreak \begin{tabularx}{8.4cm}{x{2.204 cm} x{2.736 cm} x{2.66 cm} } \SetRowColor{DarkBackground} \mymulticolumn{3}{x{8.4cm}}{\bf\textcolor{white}{Types of Microscopes (cont)}} \tn % Row 2 \SetRowColor{LightBackground} \seqsplit{Phase-contrast} microscope & Enhances contrast in unstained cells by amplifying variations in refractive index within specimen\{\{nl\}\}-especially useful for examining living, unpigmented cells & 1.Light from source\{\{nl\}\} 2.Annular ring\{\{nl\}\} 3.Condenser\{\{nl\}\} 4.Specimen\{\{nl\}\} 5.Objective\{\{nl\}\} 6.Deflected Light\{\{nl\}\} 7.Phase Ring\{\{nl\}\} 8.Eye \tn % Row Count 12 (+ 12) % Row 3 \SetRowColor{white} \seqsplit{Fluorescent} microscope (UV light) & -Shows the location of specific molecules in the cell\{\{nl\}\}-Fluorescent substances absorb UV radiation and emit visible light\{\{nl\}\}-the fluorescing molecules may occur naturally in the specimen but more often are made by tagging the molecules of interest with fluorescent dyes or antibodies\{\{nl\}\}{\emph{Fluoresceins=Apple Green}}\{\{nl\}\}{\emph{Rhodamines=Orange red}} & 1.HBO lamp house\{\{nl\}\} 2.Excitation filter\{\{nl\}\} 3.Objective\{\{nl\}\} 4.Specimen\{\{nl\}\} 5.Dichroic beam splitter\{\{nl\}\} 6.Barrier Filter\{\{nl\}\} 7.Eyepiece \tn % Row Count 38 (+ 26) \end{tabularx} \par\addvspace{1.3em} \vfill \columnbreak \begin{tabularx}{8.4cm}{x{2.204 cm} x{2.736 cm} x{2.66 cm} } \SetRowColor{DarkBackground} \mymulticolumn{3}{x{8.4cm}}{\bf\textcolor{white}{Types of Microscopes (cont)}} \tn % Row 4 \SetRowColor{LightBackground} Electron microscope & -Uses electrons to scan specimens to create and magnify images\{\{nl\}\}-Produces \seqsplit{clear/detailed} 3D images\{\{nl\}\}-Specimen has to be held in place\{\{nl\}\}-Unable to view living specimen\{\{nl\}\}-up to 50,0000x magnification & 1.Electron\{\{nl\}\} 2.Condenser\{\{nl\}\} 3.Specimen\{\{nl\}\} 4.Objective lens\{\{nl\}\} 5.First image\{\{nl\}\} 6.Final image\{\{nl\}\} 7.Fluorescent screen\{\{nl\}\} 8.Eye \tn % Row Count 16 (+ 16) \hhline{>{\arrayrulecolor{DarkBackground}}---} \end{tabularx} \par\addvspace{1.3em} \begin{tabularx}{8.4cm}{x{2.64 cm} x{5.36 cm} } \SetRowColor{DarkBackground} \mymulticolumn{2}{x{8.4cm}}{\bf\textcolor{white}{Bacterial Cell Structures}} \tn % Row 0 \SetRowColor{LightBackground} \mymulticolumn{2}{x{8.4cm}}{{\bf{Outer Layers}}} \tn % Row Count 1 (+ 1) % Row 1 \SetRowColor{white} \mymulticolumn{2}{x{8.4cm}}{1. Glycocalyx (capsule,slime layer)} \tn % Row Count 2 (+ 1) % Row 2 \SetRowColor{LightBackground} 2. Cell Wall & {\emph{Gram Positive Cell Wall}}\{\{nl\}\}-Peptidoglycan\{\{nl\}\}-teichoic acid and lipoteichoic acid\{\{nl\}\}{\emph{Gram Negative Cell Wall}}\{\{nl\}\}-Outer membrane\{\{nl\}\}-{}-LPS, Lipoprotein, Porin protein\{\{nl\}\}-Peptidoglycan\{\{nl\}\}-Periplasmic space \tn % Row Count 11 (+ 9) % Row 3 \SetRowColor{white} \mymulticolumn{2}{x{8.4cm}}{{\bf{Cell Membrane}}} \tn % Row Count 12 (+ 1) % Row 4 \SetRowColor{LightBackground} Phospholipid Bilayer & -fatty acids\{\{nl\}\}-glycerol \tn % Row Count 14 (+ 2) % Row 5 \SetRowColor{white} \mymulticolumn{2}{x{8.4cm}}{{\bf{Cytoplasm}}} \tn % Row Count 15 (+ 1) % Row 6 \SetRowColor{LightBackground} \mymulticolumn{2}{x{8.4cm}}{Chromatin} \tn % Row Count 16 (+ 1) % Row 7 \SetRowColor{white} \mymulticolumn{2}{x{8.4cm}}{Ribosomes} \tn % Row Count 17 (+ 1) % Row 8 \SetRowColor{LightBackground} \mymulticolumn{2}{x{8.4cm}}{{\bf{Cell Shapes}}} \tn % Row Count 18 (+ 1) % Row 9 \SetRowColor{white} Cocci & -Circles \tn % Row Count 19 (+ 1) % Row 10 \SetRowColor{LightBackground} Bacilli & -Rods \tn % Row Count 20 (+ 1) % Row 11 \SetRowColor{white} Spirilla & -Spirals \tn % Row Count 21 (+ 1) \hhline{>{\arrayrulecolor{DarkBackground}}--} \end{tabularx} \par\addvspace{1.3em} \begin{tabularx}{8.4cm}{x{1.008 cm} x{2.016 cm} x{2.088 cm} x{2.088 cm} } \SetRowColor{DarkBackground} \mymulticolumn{4}{x{8.4cm}}{\bf\textcolor{white}{Types of Differential Stains}} \tn % Row 0 \SetRowColor{LightBackground} {\emph{Stain Type}} & {\emph{Specific Dyes}} & {\emph{Purpose}} & {\emph{Outcome}} \tn % Row Count 3 (+ 3) % Row 1 \SetRowColor{white} {\bf{Gram Stain}} & Uses crystal violet, Gram's iodine, ethanol \seqsplit{(decolorizer)} and safranin & Used to \seqsplit{distinguish} cells by cell-wall type \seqsplit{(gram-positive}, \seqsplit{gram-negative)} & \seqsplit{Gram-positive} cells stain \seqsplit{purple/violet}. \seqsplit{Gram-negative} cells stain pink. \tn % Row Count 10 (+ 7) % Row 2 \SetRowColor{LightBackground} {\bf{Acid-fast Stain}} & After staining with basic fuchsin, acid-fast bacteria resist \seqsplit{decolorization} by \seqsplit{acid-alcohol}. Non acid-fast bacteria are \seqsplit{counterstained} with methylene blue. & Used to \seqsplit{distinguish} acid-fast bacteria such as M. \seqsplit{tuberculosis}, from non-acid fast cells & Acid-fast bacteria are red; non-acid fast cells are blue. \tn % Row Count 25 (+ 15) % Row 3 \SetRowColor{white} {\bf{Endospore Stain}} & Uses heat to stain endospores with malachite green, then cell is washed and \seqsplit{counterstained} with safranin & Used to \seqsplit{distinguish} organisms with endospores from those without; used to study the endospore. & Endospores appear \seqsplit{bluish-green;} other structures appear pink to red. \tn % Row Count 35 (+ 10) \end{tabularx} \par\addvspace{1.3em} \vfill \columnbreak \begin{tabularx}{8.4cm}{x{1.008 cm} x{2.016 cm} x{2.088 cm} x{2.088 cm} } \SetRowColor{DarkBackground} \mymulticolumn{4}{x{8.4cm}}{\bf\textcolor{white}{Types of Differential Stains (cont)}} \tn % Row 4 \SetRowColor{LightBackground} {\bf{Flagella Stain}} & Flagella are coated with tannic acid or potassium alum mordant, then stained using either \seqsplit{pararosaline} or basic fuchsin & Used to view and study flagella in bacteria that have them & Flagella are visible if present \tn % Row Count 11 (+ 11) % Row 5 \SetRowColor{white} {\bf{Capsule Stain}} & Negatie staining with India ink or nigrosin is used to stain the background, leaving a clear area of the cell and the capsule. \seqsplit{Counterstaining} can be used to stain the cell while leaving the capsule clear & Used to \seqsplit{distinguish} cells with capsules from those without & Capsules appear clear or as halos if present \tn % Row Count 30 (+ 19) \hhline{>{\arrayrulecolor{DarkBackground}}----} \end{tabularx} \par\addvspace{1.3em} % That's all folks \end{multicols*} \end{document}