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% Most of the following are not required for the majority
% of cheat sheets but are needed for some symbol support.
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% Document Info
\author{Morghay123}
\pdfinfo{
  /Title (dna-isolation-and-quantitation.pdf)
  /Creator (Cheatography)
  /Author (Morghay123)
  /Subject (DNA Isolation and Quantitation Cheat Sheet)
}

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\noindent
\begin{multicols}{3}
\begin{tabulary}{5.8cm}{C}
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    \vspace{-7pt}
    {\parbox{\dimexpr\textwidth-2\fboxsep\relax}{\noindent
        \hspace*{-6pt}\includegraphics[width=5.8cm]{/web/www.cheatography.com/public/images/cheatography_logo.pdf}}
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\begin{tabulary}{11cm}{L}
    \vspace{-2pt}\large{\bf{\textcolor{DarkBackground}{\textrm{DNA Isolation and Quantitation Cheat Sheet}}}} \\
    \normalsize{by \textcolor{DarkBackground}{Morghay123} via \textcolor{DarkBackground}{\uline{cheatography.com/53154/cs/16995/}}}
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\noindent
\begin{multicols}{3}
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  \mymulticolumn{2}{p{5.377cm}}{\bf\textcolor{white}{Cheatographer}}  \\
  \vspace{-2pt}Morghay123 \\
  \uline{cheatography.com/morghay123} \\
  \end{tabulary}
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\begin{tabulary}{5.8cm}{L}
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  \mymulticolumn{1}{p{5.377cm}}{\bf\textcolor{white}{Cheat Sheet}}  \\
   \vspace{-2pt}Not Yet Published.\\
   Updated 8th September, 2018.\\
   Page {\thepage} of \pageref{LastPage}.
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  %\includegraphics[width=48px,height=48px]{dave.jpeg}
  Measure your website readability!\\
  www.readability-score.com
\end{tabulary}
\end{multicols}}




\begin{document}
\raggedright
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% from this page to resize cheat sheet text:
% www.emerson.emory.edu/services/latex/latex_169.html
\footnotesize % Small font.

\begin{multicols*}{2}

\begin{tabularx}{8.4cm}{x{3.2 cm} x{4.8 cm} }
\SetRowColor{DarkBackground}
\mymulticolumn{2}{x{8.4cm}}{\bf\textcolor{white}{Specimens in the Lab}}  \tn
% Row 0
\SetRowColor{LightBackground}
 & {\bf{Adequate for PCR}} \tn 
% Row Count 1 (+ 1)
% Row 1
\SetRowColor{white}
- Whole Blood & - Dried Blood \tn 
% Row Count 2 (+ 1)
% Row 2
\SetRowColor{LightBackground}
- Bone Marrow & - Saliva \tn 
% Row Count 3 (+ 1)
% Row 3
\SetRowColor{white}
- Tissue/ Scrapings & - Bone, Teeth \tn 
% Row Count 5 (+ 2)
% Row 4
\SetRowColor{LightBackground}
- Bacteria & - Amniotic fluid \tn 
% Row Count 6 (+ 1)
% Row 5
\SetRowColor{white}
- Fungi/Yeasts & - Hair follicles/ Hair Shafts \tn 
% Row Count 8 (+ 2)
% Row 6
\SetRowColor{LightBackground}
- Buccal Cells & - Buccal Cells \tn 
% Row Count 9 (+ 1)
% Row 7
\SetRowColor{white}
- Hair & - Cerebrospinal fluid \tn 
% Row Count 10 (+ 1)
% Row 8
\SetRowColor{LightBackground}
- Urine & - Fixed tissue \tn 
% Row Count 11 (+ 1)
% Row 9
\SetRowColor{white}
- Stool & - Stool \tn 
% Row Count 12 (+ 1)
% Row 10
\SetRowColor{LightBackground}
 & - Soil \tn 
% Row Count 13 (+ 1)
% Row 11
\SetRowColor{white}
 & - Urine \tn 
% Row Count 14 (+ 1)
\hhline{>{\arrayrulecolor{DarkBackground}}--}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{8.4cm}{x{3.6 cm} x{4.4 cm} }
\SetRowColor{DarkBackground}
\mymulticolumn{2}{x{8.4cm}}{\bf\textcolor{white}{DNA Extraction Methods}}  \tn
% Row 0
\SetRowColor{LightBackground}
\mymulticolumn{2}{x{8.4cm}}{{\bf{Organic}}} \tn 
% Row Count 1 (+ 1)
% Row 1
\SetRowColor{white}
\mymulticolumn{2}{x{8.4cm}}{{\emph{Uses organic chemical, phenol, choloroform}}} \tn 
% Row Count 2 (+ 1)
% Row 2
\SetRowColor{LightBackground}
1. Lysis (NaOH, SDS) & 2. Acidification (acetic acid, salt) \tn 
% Row Count 4 (+ 2)
% Row 3
\SetRowColor{white}
3 Extraction (phenol, chloroform) & 4. DNA precipitation (ethanol) \tn 
% Row Count 6 (+ 2)
% Row 4
\SetRowColor{LightBackground}
\mymulticolumn{2}{x{8.4cm}}{{\bf{Inorganic}}} \tn 
% Row Count 7 (+ 1)
% Row 5
\SetRowColor{white}
\mymulticolumn{2}{x{8.4cm}}{{\emph{Uses inorganic chemicals, detergent, ethylenediamine tetraacetic acid (EDTA), acetic acid, salt (salting out, spooling)}}} \tn 
% Row Count 10 (+ 3)
% Row 6
\SetRowColor{LightBackground}
1. Lysis (Tris, EDTA, SDS) & 2. Protein precipitation (sodium acetate) \tn 
% Row Count 12 (+ 2)
% Row 7
\SetRowColor{white}
\mymulticolumn{2}{x{8.4cm}}{3. DNA precipitation (isopropanol)} \tn 
% Row Count 13 (+ 1)
% Row 8
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\mymulticolumn{2}{x{8.4cm}}{{\bf{Solid phase}}} \tn 
% Row Count 14 (+ 1)
% Row 9
\SetRowColor{white}
\mymulticolumn{2}{x{8.4cm}}{{\emph{DNA is immobilized on a solid support, beads, or columns}}} \tn 
% Row Count 16 (+ 2)
% Row 10
\SetRowColor{LightBackground}
1. Lysis (supplied reagents) & 2. Acidification (supplied reagents) \tn 
% Row Count 18 (+ 2)
% Row 11
\SetRowColor{white}
3. Adsorption (low pH) & 4. Wash (supplied buffer) \tn 
% Row Count 20 (+ 2)
% Row 12
\SetRowColor{LightBackground}
\mymulticolumn{2}{x{8.4cm}}{5. Elute DNA (low salt)} \tn 
% Row Count 21 (+ 1)
% Row 13
\SetRowColor{white}
Solid-Phase Isolation & -Kits\{\{nl\}\}-Spin Columns \tn 
% Row Count 23 (+ 2)
% Row 14
\SetRowColor{LightBackground}
\mymulticolumn{2}{x{8.4cm}}{Nucleic acid binding to silica beads in the basis for many automated extraction systems} \tn 
% Row Count 25 (+ 2)
% Row 15
\SetRowColor{white}
\mymulticolumn{2}{x{8.4cm}}{Limiting specimens (fixed tissue, dried, bone)} \tn 
% Row Count 26 (+ 1)
% Row 16
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\mymulticolumn{2}{x{8.4cm}}{Rapid extration for routine testing} \tn 
% Row Count 27 (+ 1)
% Row 17
\SetRowColor{white}
\mymulticolumn{2}{x{8.4cm}}{{\bf{Crude lysis}}} \tn 
% Row Count 28 (+ 1)
% Row 18
\SetRowColor{LightBackground}
DNA does NOT need to be pure & RE digestion, gel electrophoresis, screening large amounts of DNA, some PCR, challenging specimens \tn 
% Row Count 33 (+ 5)
\end{tabularx}
\par\addvspace{1.3em}

\vfill
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\begin{tabularx}{8.4cm}{x{3.6 cm} x{4.4 cm} }
\SetRowColor{DarkBackground}
\mymulticolumn{2}{x{8.4cm}}{\bf\textcolor{white}{DNA Extraction Methods (cont)}}  \tn
% Row 19
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Proteolytic enzymes & Proteinase K (PK) \tn 
% Row Count 2 (+ 2)
% Row 20
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Chelating resins & Chelex \tn 
% Row Count 3 (+ 1)
% Row 21
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% Row Count 4 (+ 1)
% Row 22
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\mymulticolumn{2}{x{8.4cm}}{Heat tissue (hair roots, saliva, etc.) in 300 uL 5\%-20\% Chelex 100 resin} \tn 
% Row Count 6 (+ 2)
% Row 23
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\mymulticolumn{2}{x{8.4cm}}{Chelex removes multivalent cations} \tn 
% Row Count 7 (+ 1)
% Row 24
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\mymulticolumn{2}{x{8.4cm}}{DNA is in supernatant} \tn 
% Row Count 8 (+ 1)
% Row 25
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\mymulticolumn{2}{x{8.4cm}}{{\bf{Fixed tissue}}} \tn 
% Row Count 9 (+ 1)
% Row 26
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1. \textasciitilde{}20 micron sections & Macrodissect or microdissect \tn 
% Row Count 11 (+ 2)
% Row 27
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\mymulticolumn{2}{x{8.4cm}}{2. Digest (proteinase K, Tris buffer)} \tn 
% Row Count 12 (+ 1)
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\par\addvspace{1.3em}

\begin{tabularx}{8.4cm}{x{4 cm} x{4 cm} }
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\mymulticolumn{2}{x{8.4cm}}{\bf\textcolor{white}{DNA Isolation}}  \tn
% Row 0
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DNA isolation, purification and quantitation & Free of proteins, lipids, other nucleic acids \tn 
% Row Count 3 (+ 3)
% Row 1
\SetRowColor{white}
Different methods depending on specimen type & \seqsplit{????????????????????}????????????????? \tn 
% Row Count 6 (+ 3)
% Row 2
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Different methods depending on type of DNA needed & \seqsplit{????????????????????}??????????????????????? \tn 
% Row Count 9 (+ 3)
\hhline{>{\arrayrulecolor{DarkBackground}}--}
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\par\addvspace{1.3em}

\begin{tabularx}{8.4cm}{x{4.96 cm} x{3.04 cm} }
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\mymulticolumn{2}{x{8.4cm}}{\bf\textcolor{white}{DNA Quantitation}}  \tn
% Row 0
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\mymulticolumn{2}{x{8.4cm}}{{\bf{Spectrophotometry}}} \tn 
% Row Count 1 (+ 1)
% Row 1
\SetRowColor{white}
\mymulticolumn{2}{x{8.4cm}}{Instrument that allows you to measure the amount of light that is absorbed in a sample or the amount of light transmitted based on the solute in the sample} \tn 
% Row Count 5 (+ 4)
% Row 2
\SetRowColor{LightBackground}
\mymulticolumn{2}{x{8.4cm}}{Filters and/or prisms are used to filter the light to obtain a certain wavelength} \tn 
% Row Count 7 (+ 2)
% Row 3
\SetRowColor{white}
\mymulticolumn{2}{x{8.4cm}}{A= absorbance\{\{nl\}\}\%T= transmittance} \tn 
% Row Count 8 (+ 1)
% Row 4
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\mymulticolumn{2}{x{8.4cm}}{A260/A280 Ratio} \tn 
% Row Count 9 (+ 1)
% Row 5
\SetRowColor{white}
\mymulticolumn{2}{x{8.4cm}}{1.65 \textless{}= A260/A280 \textless{}= 2.50} \tn 
% Row Count 10 (+ 1)
% Row 6
\SetRowColor{LightBackground}
\mymulticolumn{2}{x{8.4cm}}{dsDNA concentration = 50 ug/mL x OD260 x dilution factor} \tn 
% Row Count 12 (+ 2)
% Row 7
\SetRowColor{white}
\mymulticolumn{2}{x{8.4cm}}{{\bf{Beer - Lambert Law}}} \tn 
% Row Count 13 (+ 1)
% Row 8
\SetRowColor{LightBackground}
\mymulticolumn{2}{x{8.4cm}}{Absorbance is directly proportionate to the concentration of the solute (DNA)} \tn 
% Row Count 15 (+ 2)
% Row 9
\SetRowColor{white}
\mymulticolumn{2}{x{8.4cm}}{Absorptivity constant - 50 for DNA} \tn 
% Row Count 16 (+ 1)
% Row 10
\SetRowColor{LightBackground}
\mymulticolumn{2}{x{8.4cm}}{- Conversion factor from optical density unit (absorbance units) to concentration} \tn 
% Row Count 18 (+ 2)
% Row 11
\SetRowColor{white}
\mymulticolumn{2}{x{8.4cm}}{- Residual phenol - bad!!} \tn 
% Row Count 19 (+ 1)
% Row 12
\SetRowColor{LightBackground}
\mymulticolumn{2}{x{8.4cm}}{{\bf{Calculations}}} \tn 
% Row Count 20 (+ 1)
% Row 13
\SetRowColor{white}
\mymulticolumn{2}{x{8.4cm}}{Usually have to dilute samples from preps to begin} \tn 
% Row Count 21 (+ 1)
% Row 14
\SetRowColor{LightBackground}
A DNA prep diluted 1:100 has an A260 reading of 0.200. Find the concentration in ug/mL & \{\{nl\}\}\{\{nl\}\}\{\{nl\}\}\{\{nl\}\} \tn 
% Row Count 25 (+ 4)
% Row 15
\SetRowColor{white}
A DNA prep diluted 1:50 has an A260 reading of 0.307. Find the concentration in ug/mL. & \{\{nl\}\}\{\{nl\}\}\{\{nl\}\}\{\{nl\}\} \tn 
% Row Count 29 (+ 4)
\hhline{>{\arrayrulecolor{DarkBackground}}--}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{8.4cm}{p{0.8 cm} p{0.8 cm} }
\SetRowColor{DarkBackground}
\mymulticolumn{2}{x{8.4cm}}{\bf\textcolor{white}{Separating out the cells}}  \tn
% Row 0
\SetRowColor{LightBackground}
\mymulticolumn{2}{x{8.4cm}}{Differetial Density gradient centrifugation} \tn 
% Row Count 1 (+ 1)
\hhline{>{\arrayrulecolor{DarkBackground}}--}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{8.4cm}{p{0.8 cm} p{0.8 cm} }
\SetRowColor{DarkBackground}
\mymulticolumn{2}{x{8.4cm}}{\bf\textcolor{white}{Breaking open the cells}}  \tn
% Row 0
\SetRowColor{LightBackground}
\mymulticolumn{2}{x{8.4cm}}{Alkaline lysis} \tn 
% Row Count 1 (+ 1)
% Row 1
\SetRowColor{white}
\mymulticolumn{2}{x{8.4cm}}{Differential lysis} \tn 
% Row Count 2 (+ 1)
\hhline{>{\arrayrulecolor{DarkBackground}}--}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{8.4cm}{p{0.8 cm} p{0.8 cm} }
\SetRowColor{DarkBackground}
\mymulticolumn{2}{x{8.4cm}}{\bf\textcolor{white}{Cell wall digestion}}  \tn
% Row 0
\SetRowColor{LightBackground}
\mymulticolumn{2}{x{8.4cm}}{Lysozyme} \tn 
% Row Count 1 (+ 1)
% Row 1
\SetRowColor{white}
\mymulticolumn{2}{x{8.4cm}}{Zymolyase} \tn 
% Row Count 2 (+ 1)
% Row 2
\SetRowColor{LightBackground}
\mymulticolumn{2}{x{8.4cm}}{Detergents} \tn 
% Row Count 3 (+ 1)
% Row 3
\SetRowColor{white}
\mymulticolumn{2}{x{8.4cm}}{Boiling} \tn 
% Row Count 4 (+ 1)
\hhline{>{\arrayrulecolor{DarkBackground}}--}
\end{tabularx}
\par\addvspace{1.3em}


% That's all folks
\end{multicols*}

\end{document}