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% Document Info
\author{MJC3}
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  /Title (hplc-analysis.pdf)
  /Creator (Cheatography)
  /Author (MJC3)
  /Subject (HPLC Analysis Cheat Sheet)
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\noindent
\begin{multicols}{3}
\begin{tabulary}{5.8cm}{C}
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    \vspace{-7pt}
    {\parbox{\dimexpr\textwidth-2\fboxsep\relax}{\noindent
        \hspace*{-6pt}\includegraphics[width=5.8cm]{/web/www.cheatography.com/public/images/cheatography_logo.pdf}}
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\begin{tabulary}{11cm}{L}
    \vspace{-2pt}\large{\bf{\textcolor{DarkBackground}{\textrm{HPLC Analysis Cheat Sheet}}}} \\
    \normalsize{by \textcolor{DarkBackground}{MJC3} via \textcolor{DarkBackground}{\uline{cheatography.com/212269/cs/46152/}}}
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\noindent
\begin{multicols}{3}
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  \mymulticolumn{2}{p{5.377cm}}{\bf\textcolor{white}{Cheatographer}}  \\
  \vspace{-2pt}MJC3 \\
  \uline{cheatography.com/mjc3} \\
  \end{tabulary}
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\columnbreak
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  \mymulticolumn{1}{p{5.377cm}}{\bf\textcolor{white}{Cheat Sheet}}  \\
   \vspace{-2pt}Published 18th April, 2025.\\
   Updated 18th April, 2025.\\
   Page {\thepage} of \pageref{LastPage}.
\end{tabulary}
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  \mymulticolumn{1}{p{5.377cm}}{\bf\textcolor{white}{Sponsor}}  \\
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  \vspace{-5pt}
  %\includegraphics[width=48px,height=48px]{dave.jpeg}
  Measure your website readability!\\
  www.readability-score.com
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\begin{document}
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\begin{multicols*}{3}

\begin{tabularx}{5.377cm}{p{0.4977 cm} p{0.4977 cm} }
\SetRowColor{DarkBackground}
\mymulticolumn{2}{x{5.377cm}}{\bf\textcolor{white}{Overview}}  \tn
% Row 0
\SetRowColor{LightBackground}
\mymulticolumn{2}{x{5.377cm}}{} \tn 
% Row Count 0 (+ 0)
\hhline{>{\arrayrulecolor{DarkBackground}}--}
\SetRowColor{LightBackground}
\mymulticolumn{2}{x{5.377cm}}{1.{\bf{Weigh tablet}}– Record to 4 significant figures (e.g., 1.000 g). \newline 2.{\bf{Grind tablet}} to fine powder. \newline 3.{\bf{Transfer}} powder to 10 mL volumetric flask. \newline 4.{\bf{Add \textasciitilde{}5 mL mobile phase}}, do not shake. \newline 5.{\bf{Sonicate}} for 5 mins. \newline 6.{\bf{Top up}} to 10 mL mark with mobile phase, then mix. \newline 7.{\bf{Filter}} into a beaker using syringe + filter disc. \newline 8.{\bf{Pipette}} 50 μL into 20 mL flask. \newline 9.{\bf{Fill}} to 20 mL with mobile phase, mix. \newline 10.{\bf{Transfer}} \textasciitilde{}1 mL to HPLC vial for analysis.}  \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}--}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{p{0.4977 cm} p{0.4977 cm} }
\SetRowColor{DarkBackground}
\mymulticolumn{2}{x{5.377cm}}{\bf\textcolor{white}{Transfer TP to volumetric and add MP}}  \tn
% Row 0
\SetRowColor{LightBackground}
\mymulticolumn{2}{x{5.377cm}}{1.Transfer your tablet powder into the {\bf{10 mL volumetric flask.}}} \tn 
% Row Count 2 (+ 2)
% Row 1
\SetRowColor{white}
\mymulticolumn{2}{x{5.377cm}}{2.Use a small amount of mobile phase (1-2 mL) to wash any powder left on the weighing boat into the flask.} \tn 
% Row Count 5 (+ 3)
% Row 2
\SetRowColor{LightBackground}
\mymulticolumn{2}{x{5.377cm}}{3.Add approximately {\bf{5mL of the mobile phase}} to the volumetric flask.} \tn 
% Row Count 7 (+ 2)
% Row 3
\SetRowColor{white}
\mymulticolumn{2}{x{5.377cm}}{4.{\bf{Do not stir or shake the flask at this step!}}} \tn 
% Row Count 8 (+ 1)
% Row 4
\SetRowColor{LightBackground}
\mymulticolumn{2}{x{5.377cm}}{5.Sonicate the flask for 5 minutes} \tn 
% Row Count 9 (+ 1)
% Row 5
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\mymulticolumn{2}{x{5.377cm}}{6.While your sample is in the sonicator you can start to prepare your next sample (if required).} \tn 
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% Row 6
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\mymulticolumn{2}{x{5.377cm}}{7.{\bf{The mobile phase is irritant}} so please wear gloves and goggles. Wash off immediately if it comes into contact with skin.} \tn 
% Row Count 14 (+ 3)
\hhline{>{\arrayrulecolor{DarkBackground}}--}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{p{0.4977 cm} p{0.4977 cm} }
\SetRowColor{DarkBackground}
\mymulticolumn{2}{x{5.377cm}}{\bf\textcolor{white}{Full Method}}  \tn
% Row 0
\SetRowColor{LightBackground}
\mymulticolumn{2}{x{5.377cm}}{1. Put on gloves and goggles} \tn 
% Row Count 1 (+ 1)
% Row 1
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\mymulticolumn{2}{x{5.377cm}}{2.Weigh the tablet – record to 4 significant figures.} \tn 
% Row Count 3 (+ 2)
% Row 2
\SetRowColor{LightBackground}
\mymulticolumn{2}{x{5.377cm}}{3.Calculate the required amount of powdered tablet that contains 200 mg paracetamol} \tn 
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% Row 3
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\mymulticolumn{2}{x{5.377cm}}{4.Crush the tablet (try to keep powder towards centre of the bowl)} \tn 
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% Row 4
\SetRowColor{LightBackground}
\mymulticolumn{2}{x{5.377cm}}{5.Tare the balance with the weighing boat. Transfer as accurately as possible the calculated amount of powder to the weighing boat.} \tn 
% Row Count 10 (+ 3)
% Row 5
\SetRowColor{white}
\mymulticolumn{2}{x{5.377cm}}{6.Add the powder to the 10 mL flask, wash any residual power into the flask with mobile phase} \tn 
% Row Count 12 (+ 2)
% Row 6
\SetRowColor{LightBackground}
\mymulticolumn{2}{x{5.377cm}}{7.FILL THE FLASK HALFWAY with approximately 5 mL of mobile phase and do not shake the content!} \tn 
% Row Count 14 (+ 2)
% Row 7
\SetRowColor{white}
\mymulticolumn{2}{x{5.377cm}}{8.Take your sample to the sonicator.} \tn 
% Row Count 15 (+ 1)
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\mymulticolumn{2}{x{5.377cm}}{9.Once sonicated, fill your flask to the 10 mL line and mix it.} \tn 
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% Row 9
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\mymulticolumn{2}{x{5.377cm}}{10.Filter your sample and put 50 μL in the 20 mL flask using a mechanical pipettor.} \tn 
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% Row 10
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\mymulticolumn{2}{x{5.377cm}}{11.Fill the 20 mL flask to the line with mobile phase, mix and transfer to HPLC vial.} \tn 
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\begin{tabularx}{5.377cm}{x{1.24425 cm} x{3.73275 cm} }
\SetRowColor{DarkBackground}
\mymulticolumn{2}{x{5.377cm}}{\bf\textcolor{white}{Dilution in 20ml flask}}  \tn
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{\bf{TRANSFER:}} & Collect 50 μL of filtrate and transfer into the 20 mL volumetric flask. \tn 
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\mymulticolumn{2}{x{5.377cm}}{1. Put a tip on the pipettor} \tn 
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\mymulticolumn{2}{x{5.377cm}}{2.Push the pipette plunger down to the first stop position and hold.} \tn 
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\mymulticolumn{2}{x{5.377cm}}{3.Insert the tip into the filtered solution, draw plunger back to the top, once the tip is filled take it out of the solution.} \tn 
% Row Count 9 (+ 3)
% Row 4
\SetRowColor{LightBackground}
\mymulticolumn{2}{x{5.377cm}}{4.Push the pipette plunger down all the way to the 2nd stop position to release all the solution into the 20 mL flask.} \tn 
% Row Count 12 (+ 3)
% Row 5
\SetRowColor{white}
{\bf{FILL:}} & Fill the flask up to 20 mL line with the mobile phase \tn 
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{\bf{MIX:}} & Mix thoroughly \tn 
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{\bf{TRANSFER:}} & Transfer approximately 1 mL of the solution to the HPLC vial using the pipettor. \tn 
% Row Count 18 (+ 3)
\hhline{>{\arrayrulecolor{DarkBackground}}--}
\SetRowColor{LightBackground}
\mymulticolumn{2}{x{5.377cm}}{{\bf{Note:}} as you are filling the flask make sure you are looking at the line at the {\bf{eye level}} to avoid parallax}  \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}--}
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\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{x{2.4885 cm} x{2.4885 cm} }
\SetRowColor{DarkBackground}
\mymulticolumn{2}{x{5.377cm}}{\bf\textcolor{white}{Weigh and Grind tablet}}  \tn
% Row 0
\SetRowColor{LightBackground}
\mymulticolumn{2}{x{5.377cm}}{{\bf{WEIGHING:}} tare the balance with the weighing boat on it, then add 1 tablet and record this value to {\bf{4 significant figures}}, e.g., 1 g = 1.000 g or 0.12 g = 0.1200 g} \tn 
% Row Count 4 (+ 4)
% Row 1
\SetRowColor{white}
{\bf{CALCULATION:}} : Using this equation calculate the amount of powder required & 𝑴𝒂𝒔𝒔 \seqsplit{𝒓𝒆𝒒𝒖𝒊𝒓𝒆𝒅=}  (𝑴𝒂𝒔𝒔 𝒐𝒇 𝒕𝒉𝒆 \seqsplit{𝒕𝒂𝒃𝒍𝒆𝒕} )/(𝟐𝟓𝟎 𝒎𝒈)  𝒙 𝟐𝟎𝟎 𝒎𝒈 \tn 
% Row Count 13 (+ 9)
% Row 2
\SetRowColor{LightBackground}
\mymulticolumn{2}{x{5.377cm}}{*Mass of the tablet is the actual value weighed and recorded which indicates the actual amount in the weighing boat.} \tn 
% Row Count 16 (+ 3)
% Row 3
\SetRowColor{white}
\mymulticolumn{2}{x{5.377cm}}{{\bf{GRINDING:}} when crushing the tablet try not to spread the powder onto the walls as this will make collection harder for the next stage} \tn 
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\hhline{>{\arrayrulecolor{DarkBackground}}--}
\end{tabularx}
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\begin{tabularx}{5.377cm}{x{0.89586 cm} x{4.08114 cm} }
\SetRowColor{DarkBackground}
\mymulticolumn{2}{x{5.377cm}}{\bf\textcolor{white}{Fill up to the 10 mL line and mix.}}  \tn
% Row 0
\SetRowColor{LightBackground}
\mymulticolumn{2}{x{5.377cm}}{Fill up to 10 mL line with mobile phase} \tn 
% Row Count 1 (+ 1)
% Row 1
\SetRowColor{white}
\mymulticolumn{2}{x{5.377cm}}{Now you can mix it by shaking.} \tn 
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% Row 2
\SetRowColor{LightBackground}
{\bf{Note:}} & Note: as you are filling the flask make sure you are looking at the line at the eye level to avoid parallax. \tn 
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\hhline{>{\arrayrulecolor{DarkBackground}}--}
\SetRowColor{LightBackground}
\mymulticolumn{2}{x{5.377cm}}{Filter the solution  \newline  \newline 1.Transfer the content of the 10 mL flask into the larger beaker. \newline 2.Draw up some of the solution into the syringe without the filter attached. \newline 3.Screw the filter disc on the syringe \newline 4.Filter the solution into the small beaker. \newline  \newline Note: the syringe is quite stiff with the filter disk on, so don't be afraid to push hard.}  \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}--}
\end{tabularx}
\par\addvspace{1.3em}


% That's all folks
\end{multicols*}

\end{document}