\documentclass[10pt,a4paper]{article}

% Packages
\usepackage{fancyhdr}           % For header and footer
\usepackage{multicol}           % Allows multicols in tables
\usepackage{tabularx}           % Intelligent column widths
\usepackage{tabulary}           % Used in header and footer
\usepackage{hhline}             % Border under tables
\usepackage{graphicx}           % For images
\usepackage{xcolor}             % For hex colours
%\usepackage[utf8x]{inputenc}    % For unicode character support
\usepackage[T1]{fontenc}        % Without this we get weird character replacements
\usepackage{colortbl}           % For coloured tables
\usepackage{setspace}           % For line height
\usepackage{lastpage}           % Needed for total page number
\usepackage{seqsplit}           % Splits long words.
%\usepackage{opensans}          % Can't make this work so far. Shame. Would be lovely.
\usepackage[normalem]{ulem}     % For underlining links
% Most of the following are not required for the majority
% of cheat sheets but are needed for some symbol support.
\usepackage{amsmath}            % Symbols
\usepackage{MnSymbol}           % Symbols
\usepackage{wasysym}            % Symbols
%\usepackage[english,german,french,spanish,italian]{babel}              % Languages

% Document Info
\author{mjb}
\pdfinfo{
  /Title (enzymes.pdf)
  /Creator (Cheatography)
  /Author (mjb)
  /Subject (Enzymes Cheat Sheet)
}

% Lengths and widths
\addtolength{\textwidth}{6cm}
\addtolength{\textheight}{-1cm}
\addtolength{\hoffset}{-3cm}
\addtolength{\voffset}{-2cm}
\setlength{\tabcolsep}{0.2cm} % Space between columns
\setlength{\headsep}{-12pt} % Reduce space between header and content
\setlength{\headheight}{85pt} % If less, LaTeX automatically increases it
\renewcommand{\footrulewidth}{0pt} % Remove footer line
\renewcommand{\headrulewidth}{0pt} % Remove header line
\renewcommand{\seqinsert}{\ifmmode\allowbreak\else\-\fi} % Hyphens in seqsplit
% This two commands together give roughly
% the right line height in the tables
\renewcommand{\arraystretch}{1.3}
\onehalfspacing

% Commands
\newcommand{\SetRowColor}[1]{\noalign{\gdef\RowColorName{#1}}\rowcolor{\RowColorName}} % Shortcut for row colour
\newcommand{\mymulticolumn}[3]{\multicolumn{#1}{>{\columncolor{\RowColorName}}#2}{#3}} % For coloured multi-cols
\newcolumntype{x}[1]{>{\raggedright}p{#1}} % New column types for ragged-right paragraph columns
\newcommand{\tn}{\tabularnewline} % Required as custom column type in use

% Font and Colours
\definecolor{HeadBackground}{HTML}{333333}
\definecolor{FootBackground}{HTML}{666666}
\definecolor{TextColor}{HTML}{333333}
\definecolor{DarkBackground}{HTML}{A3A3A3}
\definecolor{LightBackground}{HTML}{F3F3F3}
\renewcommand{\familydefault}{\sfdefault}
\color{TextColor}

% Header and Footer
\pagestyle{fancy}
\fancyhead{} % Set header to blank
\fancyfoot{} % Set footer to blank
\fancyhead[L]{
\noindent
\begin{multicols}{3}
\begin{tabulary}{5.8cm}{C}
    \SetRowColor{DarkBackground}
    \vspace{-7pt}
    {\parbox{\dimexpr\textwidth-2\fboxsep\relax}{\noindent
        \hspace*{-6pt}\includegraphics[width=5.8cm]{/web/www.cheatography.com/public/images/cheatography_logo.pdf}}
    }
\end{tabulary}
\columnbreak
\begin{tabulary}{11cm}{L}
    \vspace{-2pt}\large{\bf{\textcolor{DarkBackground}{\textrm{Enzymes Cheat Sheet}}}} \\
    \normalsize{by \textcolor{DarkBackground}{mjb} via \textcolor{DarkBackground}{\uline{cheatography.com/128288/cs/36078/}}}
\end{tabulary}
\end{multicols}}

\fancyfoot[L]{ \footnotesize
\noindent
\begin{multicols}{3}
\begin{tabulary}{5.8cm}{LL}
  \SetRowColor{FootBackground}
  \mymulticolumn{2}{p{5.377cm}}{\bf\textcolor{white}{Cheatographer}}  \\
  \vspace{-2pt}mjb \\
  \uline{cheatography.com/mjb} \\
  \end{tabulary}
\vfill
\columnbreak
\begin{tabulary}{5.8cm}{L}
  \SetRowColor{FootBackground}
  \mymulticolumn{1}{p{5.377cm}}{\bf\textcolor{white}{Cheat Sheet}}  \\
   \vspace{-2pt}Not Yet Published.\\
   Updated 13th December, 2022.\\
   Page {\thepage} of \pageref{LastPage}.
\end{tabulary}
\vfill
\columnbreak
\begin{tabulary}{5.8cm}{L}
  \SetRowColor{FootBackground}
  \mymulticolumn{1}{p{5.377cm}}{\bf\textcolor{white}{Sponsor}}  \\
  \SetRowColor{white}
  \vspace{-5pt}
  %\includegraphics[width=48px,height=48px]{dave.jpeg}
  Measure your website readability!\\
  www.readability-score.com
\end{tabulary}
\end{multicols}}




\begin{document}
\raggedright
\raggedcolumns

% Set font size to small. Switch to any value
% from this page to resize cheat sheet text:
% www.emerson.emory.edu/services/latex/latex_169.html
\footnotesize % Small font.

\begin{multicols*}{3}

\begin{tabularx}{5.377cm}{x{2.4885 cm} x{2.4885 cm} }
\SetRowColor{DarkBackground}
\mymulticolumn{2}{x{5.377cm}}{\bf\textcolor{white}{General Properties of Enzymes}}  \tn
% Row 0
\SetRowColor{LightBackground}
How enzymes differ from ordinary chemical catalysts & faster, milder conditions, specific, regulatable \tn 
% Row Count 3 (+ 3)
% Row 1
\SetRowColor{white}
Class 1: Oxidoreductase & Catalyze Redox Reactions \tn 
% Row Count 5 (+ 2)
% Row 2
\SetRowColor{LightBackground}
Class 2: Transferases & Transfer of functional groups \tn 
% Row Count 7 (+ 2)
% Row 3
\SetRowColor{white}
Class 3: Hydrolases & Hydrolysis Reactions \tn 
% Row Count 8 (+ 1)
% Row 4
\SetRowColor{LightBackground}
Class 4: Lyases & Group elimination to form double bonds \tn 
% Row Count 10 (+ 2)
% Row 5
\SetRowColor{white}
Class 5: Isomerases & Isomerization \tn 
% Row Count 11 (+ 1)
% Row 6
\SetRowColor{LightBackground}
Class 6: Ligases & Bond formation using ATP hydrolysis \tn 
% Row Count 13 (+ 2)
% Row 7
\SetRowColor{white}
Cofactors & Can be metal ions or coenzymes. Substances that increase the rate of enzymes in their reactions. \tn 
% Row Count 18 (+ 5)
% Row 8
\SetRowColor{LightBackground}
Coenzymes & Are chemically changed, organic, must be regenerated. Can be cosubstrates or prosthetic groups. \tn 
% Row Count 23 (+ 5)
% Row 9
\SetRowColor{white}
Cosubstrates & dissociate from enzyme \tn 
% Row Count 25 (+ 2)
% Row 10
\SetRowColor{LightBackground}
Prosthetic groups & permanently associated with enzyme \tn 
% Row Count 27 (+ 2)
% Row 11
\SetRowColor{white}
Free energy of activation ΔG⁺⁺ & free energy of transition state minus free energy of reactants. When this variable is larger, the reaction is slower. \tn 
% Row Count 33 (+ 6)
\hhline{>{\arrayrulecolor{DarkBackground}}--}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{x{1.84149 cm} x{3.13551 cm} }
\SetRowColor{DarkBackground}
\mymulticolumn{2}{x{5.377cm}}{\bf\textcolor{white}{Enzyme Inhibition}}  \tn
% Row 0
\SetRowColor{LightBackground}
Competitive Inhibitor & Binds at substrate binding site. Reduces concentration of free enzyme available for substrate. Ex: Aspartic Protease \tn 
% Row Count 5 (+ 5)
% Row 1
\SetRowColor{white}
Uncompetitive Inhibitor & The inhibitor binds to the enzyme-substrate complex but not to the free enzyme. Distorts SUBSTRATE OCCUPIED active site. \tn 
% Row Count 10 (+ 5)
% Row 2
\SetRowColor{LightBackground}
\seqsplit{Mixed/Noncompetitive} & Compounds that bind to the free enzyme AND to the enzyme-substrate complex \tn 
% Row Count 13 (+ 3)
\hhline{>{\arrayrulecolor{DarkBackground}}--}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{x{2.4885 cm} x{2.4885 cm} }
\SetRowColor{DarkBackground}
\mymulticolumn{2}{x{5.377cm}}{\bf\textcolor{white}{Catalytic Mechanisms}}  \tn
% Row 0
\SetRowColor{LightBackground}
1. Acid Base Catalysis & Ex: RNase, activity is affected by pH \tn 
% Row Count 2 (+ 2)
% Row 1
\SetRowColor{white}
Acid Catalysis & enzyme gives substrate a proton \tn 
% Row Count 4 (+ 2)
% Row 2
\SetRowColor{LightBackground}
Base Catalysis & enzyme takes proton from substrate \tn 
% Row Count 6 (+ 2)
% Row 3
\SetRowColor{white}
Amino acids that act as acids & Asp, Glu \tn 
% Row Count 8 (+ 2)
% Row 4
\SetRowColor{LightBackground}
Amino acids that act as bases & Arg, Lys, His \tn 
% Row Count 10 (+ 2)
% Row 5
\SetRowColor{white}
2. Covalent Catalysis & Nucleophilic attack on substrate by enzyme, resulting in temporary covalent bond formation \tn 
% Row Count 15 (+ 5)
% Row 6
\SetRowColor{LightBackground}
Nucleophilic groups & Have electrons, want proton \tn 
% Row Count 17 (+ 2)
% Row 7
\SetRowColor{white}
Electrophilic groups & Have protons, want electrons \tn 
% Row Count 19 (+ 2)
% Row 8
\SetRowColor{LightBackground}
3. Catalysis through proximity and orientation & bring substrates into contact, freeze out relative rotational and translational motions in transition state \tn 
% Row Count 25 (+ 6)
% Row 9
\SetRowColor{white}
4. Catalysis through binding transition state & strained version of substrate fits in enzyme better than unstrained substrate \tn 
% Row Count 29 (+ 4)
% Row 10
\SetRowColor{LightBackground}
 & This implies that you can inhibit with a transition state analog \tn 
% Row Count 33 (+ 4)
\end{tabularx}
\par\addvspace{1.3em}

\vfill
\columnbreak
\begin{tabularx}{5.377cm}{x{2.4885 cm} x{2.4885 cm} }
\SetRowColor{DarkBackground}
\mymulticolumn{2}{x{5.377cm}}{\bf\textcolor{white}{Catalytic Mechanisms (cont)}}  \tn
% Row 11
\SetRowColor{LightBackground}
5 Metal ion catalysis & the unique electronic properties of the metal ion facilitate the reaction. \tn 
% Row Count 4 (+ 4)
% Row 12
\SetRowColor{white}
Serine Proteases & catalyze peptide bond hydrolysis (breakage) in target proteins. \tn 
% Row Count 8 (+ 4)
% Row 13
\SetRowColor{LightBackground}
 & proximity and orientation effects, acid–base catalysis, covalent catalysis, electrostatic catalysis, and transition state stabilization. \tn 
% Row Count 15 (+ 7)
% Row 14
\SetRowColor{white}
Chymotrypsin & Binds Bulky hydrophobic side chain, cleaved by trypsin \tn 
% Row Count 18 (+ 3)
% Row 15
\SetRowColor{LightBackground}
Trypsin & Binds positively charged side chain, cleaved by enteropeptidase \tn 
% Row Count 22 (+ 4)
% Row 16
\SetRowColor{white}
Elastase & Binds neutral, small side chains, cleaved by trypsin \tn 
% Row Count 25 (+ 3)
\hhline{>{\arrayrulecolor{DarkBackground}}--}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{x{2.28942 cm} x{2.68758 cm} }
\SetRowColor{DarkBackground}
\mymulticolumn{2}{x{5.377cm}}{\bf\textcolor{white}{Reaction Kinetics}}  \tn
% Row 0
\SetRowColor{LightBackground}
To find the mechanism of an enzyme & measure kinetics, use X-ray crystalography \tn 
% Row Count 2 (+ 2)
% Row 1
\SetRowColor{white}
K, rate constant & the rate of an elementary reaction is proportional to the frequency by which the reacting molecules come together. The proportionality constant is k \tn 
% Row Count 10 (+ 8)
% Row 2
\SetRowColor{LightBackground}
V, Reaction Velocity & the instantaneous rate of appearance of product (or disappearance of substrate). \tn 
% Row Count 14 (+ 4)
% Row 3
\SetRowColor{white}
Order & the molecularity of a reaction, i.e. the number of molecules that must simultaneously collide to generate a product. \tn 
% Row Count 20 (+ 6)
% Row 4
\SetRowColor{LightBackground}
First Order & A -\textgreater{} P, linear \tn 
% Row Count 21 (+ 1)
% Row 5
\SetRowColor{white}
Second Order & A + A -\textgreater{} P \tn 
% Row Count 22 (+ 1)
% Row 6
\SetRowColor{LightBackground}
Third Order & A + B -\textgreater{} P \tn 
% Row Count 23 (+ 1)
% Row 7
\SetRowColor{white}
KS & measure of enzyme affinity for its substrate \tn 
% Row Count 26 (+ 3)
% Row 8
\SetRowColor{LightBackground}
Km & the concentration of substrate which permits the enzyme to achieve half Vmax \tn 
% Row Count 30 (+ 4)
\end{tabularx}
\par\addvspace{1.3em}

\vfill
\columnbreak
\begin{tabularx}{5.377cm}{x{2.28942 cm} x{2.68758 cm} }
\SetRowColor{DarkBackground}
\mymulticolumn{2}{x{5.377cm}}{\bf\textcolor{white}{Reaction Kinetics (cont)}}  \tn
% Row 9
\SetRowColor{LightBackground}
Bisubstrate reactiona & usually group transfer reactions \tn 
% Row Count 2 (+ 2)
% Row 10
\SetRowColor{white}
Sequential reactions & All substrates must combine with the enzyme before reaction can take place and products released. \tn 
% Row Count 7 (+ 5)
% Row 11
\SetRowColor{LightBackground}
Ordered & order matters \tn 
% Row Count 8 (+ 1)
% Row 12
\SetRowColor{white}
Random & order does not matter \tn 
% Row Count 9 (+ 1)
% Row 13
\SetRowColor{LightBackground}
Ping Pong Reaction & One or more products are released before all substrates have been added. • The two substrates do not encounter one another on the enzyme surface. \tn 
% Row Count 16 (+ 7)
\hhline{>{\arrayrulecolor{DarkBackground}}--}
\end{tabularx}
\par\addvspace{1.3em}


% That's all folks
\end{multicols*}

\end{document}