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\usepackage{setspace}           % For line height
\usepackage{lastpage}           % Needed for total page number
\usepackage{seqsplit}           % Splits long words.
%\usepackage{opensans}          % Can't make this work so far. Shame. Would be lovely.
\usepackage[normalem]{ulem}     % For underlining links
% Most of the following are not required for the majority
% of cheat sheets but are needed for some symbol support.
\usepackage{amsmath}            % Symbols
\usepackage{MnSymbol}           % Symbols
\usepackage{wasysym}            % Symbols
%\usepackage[english,german,french,spanish,italian]{babel}              % Languages

% Document Info
\author{iplorip}
\pdfinfo{
  /Title (enzymes.pdf)
  /Creator (Cheatography)
  /Author (iplorip)
  /Subject (Enzymes Cheat Sheet)
}

% Lengths and widths
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% Header and Footer
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\fancyhead{} % Set header to blank
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\fancyhead[L]{
\noindent
\begin{multicols}{3}
\begin{tabulary}{5.8cm}{C}
    \SetRowColor{DarkBackground}
    \vspace{-7pt}
    {\parbox{\dimexpr\textwidth-2\fboxsep\relax}{\noindent
        \hspace*{-6pt}\includegraphics[width=5.8cm]{/web/www.cheatography.com/public/images/cheatography_logo.pdf}}
    }
\end{tabulary}
\columnbreak
\begin{tabulary}{11cm}{L}
    \vspace{-2pt}\large{\bf{\textcolor{DarkBackground}{\textrm{Enzymes Cheat Sheet}}}} \\
    \normalsize{by \textcolor{DarkBackground}{iplorip} via \textcolor{DarkBackground}{\uline{cheatography.com/45365/cs/13354/}}}
\end{tabulary}
\end{multicols}}

\fancyfoot[L]{ \footnotesize
\noindent
\begin{multicols}{3}
\begin{tabulary}{5.8cm}{LL}
  \SetRowColor{FootBackground}
  \mymulticolumn{2}{p{5.377cm}}{\bf\textcolor{white}{Cheatographer}}  \\
  \vspace{-2pt}iplorip \\
  \uline{cheatography.com/iplorip} \\
  \end{tabulary}
\vfill
\columnbreak
\begin{tabulary}{5.8cm}{L}
  \SetRowColor{FootBackground}
  \mymulticolumn{1}{p{5.377cm}}{\bf\textcolor{white}{Cheat Sheet}}  \\
   \vspace{-2pt}Published 31st October, 2017.\\
   Updated 31st October, 2017.\\
   Page {\thepage} of \pageref{LastPage}.
\end{tabulary}
\vfill
\columnbreak
\begin{tabulary}{5.8cm}{L}
  \SetRowColor{FootBackground}
  \mymulticolumn{1}{p{5.377cm}}{\bf\textcolor{white}{Sponsor}}  \\
  \SetRowColor{white}
  \vspace{-5pt}
  %\includegraphics[width=48px,height=48px]{dave.jpeg}
  Measure your website readability!\\
  www.readability-score.com
\end{tabulary}
\end{multicols}}




\begin{document}
\raggedright
\raggedcolumns

% Set font size to small. Switch to any value
% from this page to resize cheat sheet text:
% www.emerson.emory.edu/services/latex/latex_169.html
\footnotesize % Small font.

\begin{multicols*}{3}

\begin{tabularx}{5.377cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{5.377cm}}{\bf\textcolor{white}{Lactate dehydrogenase}}  \tn
\SetRowColor{white}
\mymulticolumn{1}{x{5.377cm}}{lactate + NAD+ -{}-\textgreater{} pyruvate + NADH + H+ \newline % Row Count 1 (+ 1)
tetrameric \newline % Row Count 2 (+ 1)
H-form: aerobic , heart L-{}-\textgreater{}P \newline % Row Count 3 (+ 1)
M-form: anaerobic , muscle/liver  P-{}-\textgreater{}L \newline % Row Count 4 (+ 1)
Reagents: Lactate,NAD+,Oxidized PMS, Oxidized NBT \newline % Row Count 5 (+ 1)
Specific Activity stain \newline % Row Count 6 (+ 1)
LDH-1: pyruvate inhibition  \newline % Row Count 7 (+ 1)
LDH-1/2: 2-hydroxybutyrate as S \newline % Row Count 8 (+ 1)
LDH-4/5: greater heat stability% Row Count 9 (+ 1)
} \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{5.377cm}}{\bf\textcolor{white}{Creatine Kinase}}  \tn
\SetRowColor{white}
\mymulticolumn{1}{x{5.377cm}}{Creatine + ATP \textless{}-{}-\textgreater{} creatine phosphate + ADP + H+ \newline % Row Count 1 (+ 1)
Dimeric \newline % Row Count 2 (+ 1)
Cardiac: MM+ {\bf{MB}} (Myocardial infarction) \newline % Row Count 3 (+ 1)
Skeletal: MM \newline % Row Count 4 (+ 1)
Brain: BB% Row Count 5 (+ 1)
} \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{5.377cm}}{\bf\textcolor{white}{Chymosin (Rennin)}}  \tn
\SetRowColor{white}
\mymulticolumn{1}{x{5.377cm}}{Aspartic protease \newline % Row Count 1 (+ 1)
Cleave single peptide bond,  \newline % Row Count 2 (+ 1)
release acidic C-terminal peptide \newline % Row Count 3 (+ 1)
Ca induced aggregation of modified casein micelle-{}-\textgreater{} precipitate as curd% Row Count 5 (+ 2)
} \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{5.377cm}}{\bf\textcolor{white}{Affinity label}}  \tn
\SetRowColor{white}
\mymulticolumn{1}{x{5.377cm}}{Specific \& Irreversible inhibitor \newline % Row Count 1 (+ 1)
Specificity group \& reactive group \newline % Row Count 2 (+ 1)
resembles substrate \newline % Row Count 3 (+ 1)
TPCK on His-57 of Chymotrypsin% Row Count 4 (+ 1)
} \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{5.377cm}}{\bf\textcolor{white}{Determination of enzyme activity}}  \tn
\SetRowColor{white}
\mymulticolumn{1}{x{5.377cm}}{NAD+ : absorbance change at 340nm \newline % Row Count 1 (+ 1)
FAD: absorbance change at 440 nm X% Row Count 2 (+ 1)
} \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{5.377cm}}{\bf\textcolor{white}{Deter}}  \tn
\SetRowColor{white}
\mymulticolumn{1}{x{5.377cm}}{Follow INITIAL rate, rate drops  \newline % Row Count 1 (+ 1)
1. substrate depletion \newline % Row Count 2 (+ 1)
2. reverse reaction \newline % Row Count 3 (+ 1)
3. product inhibition \newline % Row Count 4 (+ 1)
4. enzyme stability% Row Count 5 (+ 1)
} \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{5.377cm}}{\bf\textcolor{white}{Differentiation}}  \tn
\SetRowColor{LightBackground}
\mymulticolumn{1}{p{5.377cm}}{\vspace{1px}\centerline{\includegraphics[width=5.1cm]{/web/www.cheatography.com/public/uploads/iplorip_1509478874_SDvDD-1.png}}} \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{5.377cm}}{\bf\textcolor{white}{Homeostatsis(regulation)}}  \tn
\SetRowColor{white}
\mymulticolumn{1}{x{5.377cm}}{1. {[}S{]} control, M-M vs Cooperativity \newline % Row Count 1 (+ 1)
2. Allosteric effect \newline % Row Count 2 (+ 1)
3. {[}S{]}cycle, 2-way, 6-Phosphofructokinase \& Fructose bisphosphatase \newline % Row Count 4 (+ 2)
4. Zymogen activation \newline % Row Count 5 (+ 1)
5. Covalent modification (phosphorylation,adenylylation,myristoylation,ADP-ribosylation,methylation,acetylation,ubiquitination) \newline % Row Count 8 (+ 3)
6. Enzyme cascade \newline % Row Count 9 (+ 1)
7. Cascade amp \newline % Row Count 10 (+ 1)
8. Enzyme induction/degradation% Row Count 11 (+ 1)
} \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{5.377cm}}{\bf\textcolor{white}{Modification of amino acids}}  \tn
\SetRowColor{white}
\mymulticolumn{1}{x{5.377cm}}{Active site residues more susceptible \newline % Row Count 1 (+ 1)
Ser-195 on chymotrypsin by DIPF -{}-\textgreater{}Activity \newline % Row Count 2 (+ 1)
Modify 1\textasciicircum{}st\textasciicircum{} with {[}S{]}/{[}I{]} to protect active site, then modify again in absence \newline % Row Count 4 (+ 2)
ADH inactivated by iodoacetate more than iodoacetamide \newline % Row Count 6 (+ 2)
2AA involved: pKa \textgreater{}2 units apart:Good \newline % Row Count 7 (+ 1)
Close-{}-\textgreater{} tVmax never achieved% Row Count 8 (+ 1)
} \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{5.377cm}}{\bf\textcolor{white}{Lite Beer}}  \tn
\SetRowColor{white}
\mymulticolumn{1}{x{5.377cm}}{Barley:α-amylase cannot break down α-1,6 bond + dextrin -{}-\textgreater{} Yeast \newline % Row Count 2 (+ 2)
Glucoamylase from Aspergillus niger: break α-1,6 bond, less dextrin% Row Count 4 (+ 2)
} \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{5.377cm}}{\bf\textcolor{white}{Aspartame}}  \tn
\SetRowColor{white}
\mymulticolumn{1}{x{5.377cm}}{Thermolysin \newline % Row Count 1 (+ 1)
{\bf{L-phenylalanine}} + N-protected L-aspartate \newline % Row Count 2 (+ 1)
N: benzyloxycarbonyl% Row Count 3 (+ 1)
} \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{5.377cm}}{\bf\textcolor{white}{Catalysis}}  \tn
\SetRowColor{white}
\mymulticolumn{1}{x{5.377cm}}{1. Strain/Distortion: entropy reduction \newline % Row Count 1 (+ 1)
2. Acid-Base : carbonic anhydrase \newline % Row Count 2 (+ 1)
3. Covalent catalysis: serine protease \newline % Row Count 3 (+ 1)
4. Lower Ea: Zn\&Arg127 in carboxypeptidase A stabilize TS% Row Count 5 (+ 2)
} \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\SetRowColor{LightBackground}
\mymulticolumn{1}{x{5.377cm}}{Histidine can be both e\textasciicircum{}-\textasciicircum{} donor/acceptor \newline TS analogue: {\bf{pyrrole-2-carboxylate}} on {\emph{proline racemase}} as inhibitor \newline Abzyme: mimic Ferrochelatase}  \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{5.377cm}}{\bf\textcolor{white}{Ribozyme}}  \tn
\SetRowColor{LightBackground}
\mymulticolumn{1}{p{5.377cm}}{\vspace{1px}\centerline{\includegraphics[width=5.1cm]{/web/www.cheatography.com/public/uploads/iplorip_1509472553_L19RNA-1-min.png}}} \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\SetRowColor{LightBackground}
\mymulticolumn{1}{x{5.377cm}}{RNA less versatile (4 building blocks AUCG) \newline unable to form large non-polar region \newline nucleic acid preferred as substrate \newline RNA susceptible to hydrolysis}  \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{5.377cm}}{\bf\textcolor{white}{Organic Cofactors}}  \tn
\SetRowColor{LightBackground}
\mymulticolumn{1}{p{5.377cm}}{\vspace{1px}\centerline{\includegraphics[width=5.1cm]{/web/www.cheatography.com/public/uploads/iplorip_1509473290_organic cofactors-1.png}}} \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\SetRowColor{LightBackground}
\mymulticolumn{1}{x{5.377cm}}{Catalytic cofactor: e.g. TPP/FAD \newline Stoichiometric cofactor: cosubstrate}  \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{5.377cm}}{\bf\textcolor{white}{Radioisotope}}  \tn
\SetRowColor{white}
\mymulticolumn{1}{x{5.377cm}}{Glutamate decarboxylase \newline % Row Count 1 (+ 1)
CO2 : trap gas in alkali \newline % Row Count 2 (+ 1)
Monoamine oxidase \newline % Row Count 3 (+ 1)
R-CHO:extracted by ether after acidification (acidified R-NH2 will remain in aq phase) \newline % Row Count 5 (+ 2)
Cholinesterase \newline % Row Count 6 (+ 1)
COOH: ion exchange, importance of {\bf{label position}}% Row Count 8 (+ 2)
} \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{5.377cm}}{\bf\textcolor{white}{Scintillation Proximity Assay}}  \tn
\SetRowColor{white}
\mymulticolumn{1}{x{5.377cm}}{Radioligand stimulate bead to emit light, when in {\bf{close proximity}} \newline % Row Count 2 (+ 2)
High affinity capture system: biotinylated substrate \& streptavidin-coated beads \newline % Row Count 4 (+ 2)
NO separation needed. S or P bind to bead% Row Count 5 (+ 1)
} \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{5.377cm}}{\bf\textcolor{white}{Competitive inhibitor}}  \tn
\SetRowColor{LightBackground}
\mymulticolumn{1}{p{5.377cm}}{\vspace{1px}\centerline{\includegraphics[width=5.1cm]{/web/www.cheatography.com/public/uploads/iplorip_1509477604_Dixon-1-1.png}}} \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\SetRowColor{LightBackground}
\mymulticolumn{1}{x{5.377cm}}{Same site, mutually exclusive \newline Vmax unchanged Km increased}  \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{5.377cm}}{\bf\textcolor{white}{Non-competitive inhibitor}}  \tn
\SetRowColor{LightBackground}
\mymulticolumn{1}{p{5.377cm}}{\vspace{1px}\centerline{\includegraphics[width=5.1cm]{/web/www.cheatography.com/public/uploads/iplorip_1509477871_Non-c-1.png}}} \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\SetRowColor{LightBackground}
\mymulticolumn{1}{x{5.377cm}}{ESI present, Km unchanged, Vmax decrease,  \newline equal Ki,same \% inhib.}  \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{5.377cm}}{\bf\textcolor{white}{Pre-steady state kinetics}}  \tn
\SetRowColor{white}
\mymulticolumn{1}{x{5.377cm}}{E+S-{}-\textgreater{} ES(flurorescence) \newline % Row Count 1 (+ 1)
Stopped flow technique, follow time course of fluorescence change% Row Count 3 (+ 2)
} \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{5.377cm}}{\bf\textcolor{white}{Irreversible inhibitor}}  \tn
\SetRowColor{white}
\mymulticolumn{1}{x{5.377cm}}{Diisopropyl phosphofluoridate ({\bf{DIPF}}) modifies {\emph{serine}} on AchE% Row Count 2 (+ 2)
} \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{5.377cm}}{\bf\textcolor{white}{Hill Coefficient}}  \tn
\SetRowColor{LightBackground}
\mymulticolumn{1}{p{5.377cm}}{\vspace{1px}\centerline{\includegraphics[width=5.1cm]{/web/www.cheatography.com/public/uploads/iplorip_1509480342_Hill-1.png}}} \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\SetRowColor{LightBackground}
\mymulticolumn{1}{x{5.377cm}}{Important: Choice of {[}S{]} \newline Cooperativity: Same {\bf{site}}\&{\bf{ligand}}}  \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{5.377cm}}{\bf\textcolor{white}{Chymotrypsin}}  \tn
\SetRowColor{white}
\mymulticolumn{1}{x{5.377cm}}{Active site {\bf{Ser195,His57\&Asp102}} form charge relay system-{}-\textgreater{} high reactivity of Ser195 \newline % Row Count 2 (+ 2)
Selective for carboxyl side of aromatic or large hydrophobic residue(Met) \newline % Row Count 4 (+ 2)
Biphasic kinetics: \newline % Row Count 5 (+ 1)
1. Burst phase: covalent complex \newline % Row Count 6 (+ 1)
2. SS Phase: hydrolysis+ recovery  \newline % Row Count 7 (+ 1)
Double displacement, p-nitrophenolate% Row Count 8 (+ 1)
} \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{x{1.05271 cm} x{1.64772 cm} x{1.87657 cm} }
\SetRowColor{DarkBackground}
\mymulticolumn{3}{x{5.377cm}}{\bf\textcolor{white}{Cofactor}}  \tn
% Row 0
\SetRowColor{LightBackground}
\seqsplit{Apoenzyme} & inactive form &  \tn 
% Row Count 1 (+ 1)
% Row 1
\SetRowColor{white}
Cofactor & non-protein &  \tn 
% Row Count 2 (+ 1)
% Row 2
\SetRowColor{LightBackground}
\seqsplit{Holoenzyme} & active &  \tn 
% Row Count 4 (+ 2)
% Row 3
\SetRowColor{white}
\seqsplit{Metalloenzyme} & participate in \seqsplit{reaction(lewis} acid) & Zn in carbonic anhydrase \tn 
% Row Count 7 (+ 3)
% Row 4
\SetRowColor{LightBackground}
\seqsplit{Metalloenzyme} & stabilize transition state & Zn in \seqsplit{carboxypeptidase} A \tn 
% Row Count 9 (+ 2)
% Row 5
\SetRowColor{white}
Metal \seqsplit{activated} enzyme & maintain active conformation & K+ in pyruvate kinase \tn 
% Row Count 12 (+ 3)
% Row 6
\SetRowColor{LightBackground}
Metal \seqsplit{activated} enzyme & form substrate complex bridge & Mg in kinase \tn 
% Row Count 15 (+ 3)
% Row 7
\SetRowColor{white}
\seqsplit{Prosthetic} group & tightly bound & NH2 on pyridoxal phosphate of aspartate transaminase \tn 
% Row Count 19 (+ 4)
% Row 8
\SetRowColor{LightBackground}
Coenzyme & loosly bound &  \tn 
% Row Count 20 (+ 1)
% Row 9
\SetRowColor{white}
\seqsplit{Cosubstrate(coenzyme)} & convert to product after Rx & NAD+ \tn 
% Row Count 23 (+ 3)
\hhline{>{\arrayrulecolor{DarkBackground}}---}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{p{0.62655 cm} p{0.58478 cm} x{1.2531 cm} x{1.71257 cm} }
\SetRowColor{DarkBackground}
\mymulticolumn{4}{x{5.377cm}}{\bf\textcolor{white}{Coenzyme analogue as drug}}  \tn
% Row 0
\SetRowColor{LightBackground}
Drug & \seqsplit{Analogue} of & enzyme inhibited & MOA \& Use \tn 
% Row Count 3 (+ 3)
% Row 1
\SetRowColor{white}
\seqsplit{Sulfonamide} & PABA & \seqsplit{dihydropteroate} synthase & folic acid synthesis,Abx \tn 
% Row Count 5 (+ 2)
% Row 2
\SetRowColor{LightBackground}
\seqsplit{Methotrexate} & \seqsplit{Folate} & \seqsplit{dihydrofolate} reductase & THF synthesis,childhood leukemia \tn 
% Row Count 7 (+ 2)
\hhline{>{\arrayrulecolor{DarkBackground}}----}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{5.377cm}}{\bf\textcolor{white}{Plot}}  \tn
\SetRowColor{LightBackground}
\mymulticolumn{1}{p{5.377cm}}{\vspace{1px}\centerline{\includegraphics[width=5.1cm]{/web/www.cheatography.com/public/uploads/iplorip_1509477404_Plot-1.png}}} \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{5.377cm}}{\bf\textcolor{white}{Uncompetitive inhib.}}  \tn
\SetRowColor{white}
\mymulticolumn{1}{x{5.377cm}}{S binding to E-{}-\textgreater{} expose site for I binding \newline % Row Count 1 (+ 1)
both Km Vmax decrease to same extent, ESI present, same slope% Row Count 3 (+ 2)
} \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{5.377cm}}{\bf\textcolor{white}{Mixed inhibition}}  \tn
\SetRowColor{white}
\mymulticolumn{1}{x{5.377cm}}{Binding affinity(Ki) not the same \newline % Row Count 1 (+ 1)
Vmax decrease, Km can in/decrease% Row Count 2 (+ 1)
} \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{5.377cm}}{\bf\textcolor{white}{Suicide substrate}}  \tn
\SetRowColor{white}
\mymulticolumn{1}{x{5.377cm}}{P irreversibly bind to E \newline % Row Count 1 (+ 1)
{\bf{Deprenyl}} on MAO on Flavin prosthetic group% Row Count 2 (+ 1)
} \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{5.377cm}}{\bf\textcolor{white}{Substrate inhibition}}  \tn
\SetRowColor{white}
\mymulticolumn{1}{x{5.377cm}}{High {[}S{]} favour ESS(nonproductive binding)  \newline % Row Count 1 (+ 1)
e.g. succinate dehydrogenase (select points for drawing)% Row Count 3 (+ 2)
} \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{x{2.18988 cm} x{2.78712 cm} }
\SetRowColor{DarkBackground}
\mymulticolumn{2}{x{5.377cm}}{\bf\textcolor{white}{Single displacement Rx}}  \tn
% Row 0
\SetRowColor{LightBackground}
Random sequential & creatine kinase \tn 
% Row Count 1 (+ 1)
% Row 1
\SetRowColor{white}
Compulsory order & ADH(NAD\textasciicircum{}+\textasciicircum{} bind 1\textasciicircum{}st\textasciicircum{}) \tn 
% Row Count 2 (+ 1)
\hhline{>{\arrayrulecolor{DarkBackground}}--}
\SetRowColor{LightBackground}
\mymulticolumn{2}{x{5.377cm}}{{\bf{Ternary complex}} present}  \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}--}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{5.377cm}}{\bf\textcolor{white}{Double displacement Rx (Ping-Pong)}}  \tn
\SetRowColor{white}
\mymulticolumn{1}{x{5.377cm}}{aspartate transaminase \newline % Row Count 1 (+ 1)
aspartate+ α-ketoglutarate -{}-\textgreater{}oxaloacetate+ glutamate (NH2 displaced)% Row Count 3 (+ 2)
} \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{5.377cm}}{\bf\textcolor{white}{Isotope exchange}}  \tn
\SetRowColor{white}
\mymulticolumn{1}{x{5.377cm}}{Occurs only in double displacement; \newline % Row Count 1 (+ 1)
exception: maltose phosphorylase \newline % Row Count 2 (+ 1)
isotope from 1\textasciicircum{}st\textasciicircum{} P back to 1\textasciicircum{}st\textasciicircum{} S in absence of 2\textasciicircum{}nd\textasciicircum{} S       e.g. sucrose phosphorylase   \newline % Row Count 4 (+ 2)
Glu-Fru + Fru* \textless{}-{}-\textgreater{} Glu-Fru* + Fru% Row Count 5 (+ 1)
} \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{x{1.46464 cm} x{1.73926 cm} x{1.3731 cm} }
\SetRowColor{DarkBackground}
\mymulticolumn{3}{x{5.377cm}}{\bf\textcolor{white}{Diff. subunits of multimeric enzyme}}  \tn
% Row 0
\SetRowColor{LightBackground}
Catalytic \& Regulatory & Aspartate \seqsplit{transcarbamoylase(ATCase)} & ATP\&CTP \tn 
% Row Count 3 (+ 3)
% Row 1
\SetRowColor{white}
2\textasciicircum{}nd\textasciicircum{} unit modify specificity & Lactose synthase & \seqsplit{α-lactalbumin} \tn 
% Row Count 6 (+ 3)
% Row 2
\SetRowColor{LightBackground}
2 diff. cat. units & tryptophan \seqsplit{synthase(α2β2)} & Tunnel connect active sites \tn 
% Row Count 9 (+ 3)
\hhline{>{\arrayrulecolor{DarkBackground}}---}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{5.377cm}}{\bf\textcolor{white}{Lysozyme}}  \tn
\SetRowColor{white}
\mymulticolumn{1}{x{5.377cm}}{hydrolyze glycosidic bond bet C-1 of NAM and C-4 of NAG,  {\bf{Non-identical site}} \newline % Row Count 2 (+ 2)
Site of cleavagea: bet D\&E, distorted Ring D \newline % Row Count 3 (+ 1)
Glu-35 as acid, H\textasciicircum{}+\textasciicircum{} to O of glycosidic bond \newline % Row Count 4 (+ 1)
Carbonium cation stabilized by  \newline % Row Count 5 (+ 1)
1. -ve charge on Asp-52 \newline % Row Count 6 (+ 1)
2. half-chair formation of sugar D (strain) (resonance stabilize +charge on C-1 with O)% Row Count 8 (+ 2)
} \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}


% That's all folks
\end{multicols*}

\end{document}