\documentclass[10pt,a4paper]{article} % Packages \usepackage{fancyhdr} % For header and footer \usepackage{multicol} % Allows multicols in tables \usepackage{tabularx} % Intelligent column widths \usepackage{tabulary} % Used in header and footer \usepackage{hhline} % Border under tables \usepackage{graphicx} % For images \usepackage{xcolor} % For hex colours %\usepackage[utf8x]{inputenc} % For unicode character support \usepackage[T1]{fontenc} % Without this we get weird character replacements \usepackage{colortbl} % For coloured tables \usepackage{setspace} % For line height \usepackage{lastpage} % Needed for total page number \usepackage{seqsplit} % Splits long words. %\usepackage{opensans} % Can't make this work so far. Shame. Would be lovely. \usepackage[normalem]{ulem} % For underlining links % Most of the following are not required for the majority % of cheat sheets but are needed for some symbol support. \usepackage{amsmath} % Symbols \usepackage{MnSymbol} % Symbols \usepackage{wasysym} % Symbols %\usepackage[english,german,french,spanish,italian]{babel} % Languages % Document Info \author{no (hqneyroses)} \pdfinfo{ /Title (topic-3-biology.pdf) /Creator (Cheatography) /Author (no (hqneyroses)) /Subject (Topic 3 biology Cheat Sheet) } % Lengths and widths \addtolength{\textwidth}{6cm} \addtolength{\textheight}{-1cm} \addtolength{\hoffset}{-3cm} \addtolength{\voffset}{-2cm} \setlength{\tabcolsep}{0.2cm} % Space between columns \setlength{\headsep}{-12pt} % Reduce space between header and content \setlength{\headheight}{85pt} % If less, LaTeX automatically increases it \renewcommand{\footrulewidth}{0pt} % Remove footer line \renewcommand{\headrulewidth}{0pt} % Remove header line \renewcommand{\seqinsert}{\ifmmode\allowbreak\else\-\fi} % Hyphens in seqsplit % This two commands together give roughly % the right line height in the tables \renewcommand{\arraystretch}{1.3} \onehalfspacing % Commands \newcommand{\SetRowColor}[1]{\noalign{\gdef\RowColorName{#1}}\rowcolor{\RowColorName}} % Shortcut for row colour \newcommand{\mymulticolumn}[3]{\multicolumn{#1}{>{\columncolor{\RowColorName}}#2}{#3}} % For coloured multi-cols \newcolumntype{x}[1]{>{\raggedright}p{#1}} % New column types for ragged-right paragraph columns \newcommand{\tn}{\tabularnewline} % Required as custom column type in use % Font and Colours \definecolor{HeadBackground}{HTML}{333333} \definecolor{FootBackground}{HTML}{666666} \definecolor{TextColor}{HTML}{333333} \definecolor{DarkBackground}{HTML}{C29767} \definecolor{LightBackground}{HTML}{FBF8F5} \renewcommand{\familydefault}{\sfdefault} \color{TextColor} % Header and Footer \pagestyle{fancy} \fancyhead{} % Set header to blank \fancyfoot{} % Set footer to blank \fancyhead[L]{ \noindent \begin{multicols}{3} \begin{tabulary}{5.8cm}{C} \SetRowColor{DarkBackground} \vspace{-7pt} {\parbox{\dimexpr\textwidth-2\fboxsep\relax}{\noindent \hspace*{-6pt}\includegraphics[width=5.8cm]{/web/www.cheatography.com/public/images/cheatography_logo.pdf}} } \end{tabulary} \columnbreak \begin{tabulary}{11cm}{L} \vspace{-2pt}\large{\bf{\textcolor{DarkBackground}{\textrm{Topic 3 biology Cheat Sheet}}}} \\ \normalsize{by \textcolor{DarkBackground}{no (hqneyroses)} via \textcolor{DarkBackground}{\uline{cheatography.com/196921/cs/41510/}}} \end{tabulary} \end{multicols}} \fancyfoot[L]{ \footnotesize \noindent \begin{multicols}{3} \begin{tabulary}{5.8cm}{LL} \SetRowColor{FootBackground} \mymulticolumn{2}{p{5.377cm}}{\bf\textcolor{white}{Cheatographer}} \\ \vspace{-2pt}no (hqneyroses) \\ \uline{cheatography.com/hqneyroses} \\ \end{tabulary} \vfill \columnbreak \begin{tabulary}{5.8cm}{L} \SetRowColor{FootBackground} \mymulticolumn{1}{p{5.377cm}}{\bf\textcolor{white}{Cheat Sheet}} \\ \vspace{-2pt}Not Yet Published.\\ Updated 16th December, 2023.\\ Page {\thepage} of \pageref{LastPage}. \end{tabulary} \vfill \columnbreak \begin{tabulary}{5.8cm}{L} \SetRowColor{FootBackground} \mymulticolumn{1}{p{5.377cm}}{\bf\textcolor{white}{Sponsor}} \\ \SetRowColor{white} \vspace{-5pt} %\includegraphics[width=48px,height=48px]{dave.jpeg} Measure your website readability!\\ www.readability-score.com \end{tabulary} \end{multicols}} \begin{document} \raggedright \raggedcolumns % Set font size to small. Switch to any value % from this page to resize cheat sheet text: % www.emerson.emory.edu/services/latex/latex_169.html \footnotesize % Small font. \begin{multicols*}{4} \begin{tabularx}{3.833cm}{X} \SetRowColor{DarkBackground} \mymulticolumn{1}{x{3.833cm}}{\bf\textcolor{white}{Subheading 1}} \tn % Row 0 \SetRowColor{LightBackground} \mymulticolumn{1}{x{3.833cm}}{{\bf{EPIGENETICS AND STEM CELLS}}} \tn % Row Count 1 (+ 1) \hhline{>{\arrayrulecolor{DarkBackground}}-} \end{tabularx} \par\addvspace{1.3em} \begin{tabularx}{3.833cm}{X} \SetRowColor{DarkBackground} \mymulticolumn{1}{x{3.833cm}}{\bf\textcolor{white}{Definition of stem cell}} \tn % Row 0 \SetRowColor{LightBackground} \mymulticolumn{1}{x{3.833cm}}{An unspecialised cell which is differentiated and can give rise to specialised cells as well as divide to produce more stem cells} \tn % Row Count 3 (+ 3) \hhline{>{\arrayrulecolor{DarkBackground}}-} \end{tabularx} \par\addvspace{1.3em} \begin{tabularx}{3.833cm}{X} \SetRowColor{DarkBackground} \mymulticolumn{1}{x{3.833cm}}{\bf\textcolor{white}{Definition of totipotent stem cell}} \tn % Row 0 \SetRowColor{LightBackground} \mymulticolumn{1}{x{3.833cm}}{A cell that has the ability to differentiate into all cell types} \tn % Row Count 2 (+ 2) \hhline{>{\arrayrulecolor{DarkBackground}}-} \end{tabularx} \par\addvspace{1.3em} \begin{tabularx}{3.833cm}{X} \SetRowColor{DarkBackground} \mymulticolumn{1}{x{3.833cm}}{\bf\textcolor{white}{Definition of polygenic}} \tn % Row 0 \SetRowColor{LightBackground} \mymulticolumn{1}{x{3.833cm}}{A characteristic showing continuous variation caused by multiple genes at different loci} \tn % Row Count 2 (+ 2) \hhline{>{\arrayrulecolor{DarkBackground}}-} \end{tabularx} \par\addvspace{1.3em} \begin{tabularx}{3.833cm}{X} \SetRowColor{DarkBackground} \mymulticolumn{1}{x{3.833cm}}{\bf\textcolor{white}{Difference between tissue and organ}} \tn % Row 0 \SetRowColor{LightBackground} \mymulticolumn{1}{x{3.833cm}}{Tissue is made of one type of cell whereas an organ is made of different tissues} \tn % Row Count 2 (+ 2) \hhline{>{\arrayrulecolor{DarkBackground}}-} \end{tabularx} \par\addvspace{1.3em} \begin{tabularx}{3.833cm}{X} \SetRowColor{DarkBackground} \mymulticolumn{1}{x{3.833cm}}{\bf\textcolor{white}{How a tissue differs in structure from a system}} \tn % Row 0 \SetRowColor{LightBackground} \mymulticolumn{1}{x{3.833cm}}{• tissue contains one type of cell} \tn % Row Count 1 (+ 1) % Row 1 \SetRowColor{white} \mymulticolumn{1}{x{3.833cm}}{• a system contains (many) \{different tissues / organs \}} \tn % Row Count 3 (+ 2) \hhline{>{\arrayrulecolor{DarkBackground}}-} \end{tabularx} \par\addvspace{1.3em} \begin{tabularx}{3.833cm}{X} \SetRowColor{DarkBackground} \mymulticolumn{1}{x{3.833cm}}{\bf\textcolor{white}{Difference between t. and p. stem cells}} \tn % Row 0 \SetRowColor{LightBackground} \mymulticolumn{1}{x{3.833cm}}{1. totipotent cells can \{ give rise to / differentiate to become \} \{any cell / extra embryonic tissues / eq \}} \tn % Row Count 3 (+ 3) % Row 1 \SetRowColor{white} \mymulticolumn{1}{x{3.833cm}}{2. pluripotent cannot \{ give rise to / differentiate to become \} \{ all cells in the body / extra embryonic tissues / eq \}} \tn % Row Count 6 (+ 3) % Row 2 \SetRowColor{LightBackground} \mymulticolumn{1}{x{3.833cm}}{3. idea that only totipotent cells give rise to other totipotent cells} \tn % Row Count 8 (+ 2) % Row 3 \SetRowColor{white} \mymulticolumn{1}{x{3.833cm}}{4. idea that totipotent cells can give rise to an entire human being, pluripotent cells cannot} \tn % Row Count 10 (+ 2) \hhline{>{\arrayrulecolor{DarkBackground}}-} \end{tabularx} \par\addvspace{1.3em} \begin{tabularx}{3.833cm}{X} \SetRowColor{DarkBackground} \mymulticolumn{1}{x{3.833cm}}{\bf\textcolor{white}{Cell specialisation / differentiation}} \tn % Row 0 \SetRowColor{LightBackground} \mymulticolumn{1}{x{3.833cm}}{1. stimulus / chemical / hormone} \tn % Row Count 1 (+ 1) % Row 1 \SetRowColor{white} \mymulticolumn{1}{x{3.833cm}}{2. genes that are (active / switched on / expressed) are transcribed to produce mRNA for the active genes} \tn % Row Count 4 (+ 3) % Row 2 \SetRowColor{LightBackground} \mymulticolumn{1}{x{3.833cm}}{3. mRNA is translated to produce protein} \tn % Row Count 5 (+ 1) % Row 3 \SetRowColor{white} \mymulticolumn{1}{x{3.833cm}}{5. this protein (permanently) modifies cell OR idea that this protein determines \{ cell structure / function \}} \tn % Row Count 8 (+ 3) \hhline{>{\arrayrulecolor{DarkBackground}}-} \end{tabularx} \par\addvspace{1.3em} \begin{tabularx}{3.833cm}{X} \SetRowColor{DarkBackground} \mymulticolumn{1}{x{3.833cm}}{\bf\textcolor{white}{Becoming specialised beta cells}} \tn % Row 0 \SetRowColor{LightBackground} \mymulticolumn{1}{x{3.833cm}}{1. stimulus e.g. chemical} \tn % Row Count 1 (+ 1) % Row 1 \SetRowColor{white} \mymulticolumn{1}{x{3.833cm}}{2. some genes are \{ active / switched on / expressed \}} \tn % Row Count 3 (+ 2) % Row 2 \SetRowColor{LightBackground} \mymulticolumn{1}{x{3.833cm}}{3. transcription / mRNA produced at active genes} \tn % Row Count 4 (+ 1) % Row 3 \SetRowColor{white} \mymulticolumn{1}{x{3.833cm}}{4. mRNA is \{translated / used\} to produce} \tn % Row Count 5 (+ 1) % Row 4 \SetRowColor{LightBackground} \mymulticolumn{1}{x{3.833cm}}{5. this protein modifies cell OR idea that this protein} \tn % Row Count 7 (+ 2) \hhline{>{\arrayrulecolor{DarkBackground}}-} \end{tabularx} \par\addvspace{1.3em} \begin{tabularx}{3.833cm}{X} \SetRowColor{DarkBackground} \mymulticolumn{1}{x{3.833cm}}{\bf\textcolor{white}{Describe how cells become specialised}} \tn % Row 0 \SetRowColor{LightBackground} \mymulticolumn{1}{x{3.833cm}}{1. stimulus / chemical / hormone} \tn % Row Count 1 (+ 1) % Row 1 \SetRowColor{white} \mymulticolumn{1}{x{3.833cm}}{2. some genes are \{ active / switched on / expressed \} ;} \tn % Row Count 3 (+ 2) % Row 2 \SetRowColor{LightBackground} \mymulticolumn{1}{x{3.833cm}}{3. transcription / mRNA produced \} at active genes ;} \tn % Row Count 5 (+ 2) % Row 3 \SetRowColor{white} \mymulticolumn{1}{x{3.833cm}}{4. mRNA is \{ translated / used \} to produce \{ protein / polypeptide \} ;} \tn % Row Count 7 (+ 2) % Row 4 \SetRowColor{LightBackground} \mymulticolumn{1}{x{3.833cm}}{5. this protein (permanently) modifies cell OR idea that this protein determines \{ cell structure / function \}} \tn % Row Count 10 (+ 3) \hhline{>{\arrayrulecolor{DarkBackground}}-} \end{tabularx} \par\addvspace{1.3em} \begin{tabularx}{3.833cm}{X} \SetRowColor{DarkBackground} \mymulticolumn{1}{x{3.833cm}}{\bf\textcolor{white}{How cells become specialised}} \tn % Row 0 \SetRowColor{LightBackground} \mymulticolumn{1}{x{3.833cm}}{• chemical signal cause some genes to be activated/switched on} \tn % Row Count 2 (+ 2) % Row 1 \SetRowColor{white} \mymulticolumn{1}{x{3.833cm}}{• only activated genes are transcribed/produce mRNA} \tn % Row Count 4 (+ 2) % Row 2 \SetRowColor{LightBackground} \mymulticolumn{1}{x{3.833cm}}{• (mRNA leads to) synthesis of specific proteins which causes cell modification} \tn % Row Count 6 (+ 2) \hhline{>{\arrayrulecolor{DarkBackground}}-} \end{tabularx} \par\addvspace{1.3em} \begin{tabularx}{3.833cm}{X} \SetRowColor{DarkBackground} \mymulticolumn{1}{x{3.833cm}}{\bf\textcolor{white}{Epigenetic mod. in daughter cells}} \tn % Row 0 \SetRowColor{LightBackground} \mymulticolumn{1}{x{3.833cm}}{• genes \{ activated / deactivated \} (in stem cells)} \tn % Row Count 2 (+ 2) % Row 1 \SetRowColor{white} \mymulticolumn{1}{x{3.833cm}}{• (because of) \{ methylation of DNA / histone binding \}} \tn % Row Count 4 (+ 2) % Row 2 \SetRowColor{LightBackground} \mymulticolumn{1}{x{3.833cm}}{• (therefore) the same genes will be activated in the daughter cells} \tn % Row Count 6 (+ 2) \hhline{>{\arrayrulecolor{DarkBackground}}-} \end{tabularx} \par\addvspace{1.3em} \begin{tabularx}{3.833cm}{X} \SetRowColor{DarkBackground} \mymulticolumn{1}{x{3.833cm}}{\bf\textcolor{white}{Subheading 2}} \tn % Row 0 \SetRowColor{LightBackground} \mymulticolumn{1}{x{3.833cm}}{{\bf{EUKARYOTES AND PROKARYOTES}}} \tn % Row Count 1 (+ 1) \hhline{>{\arrayrulecolor{DarkBackground}}-} \end{tabularx} \par\addvspace{1.3em} \begin{tabularx}{3.833cm}{X} \SetRowColor{DarkBackground} \mymulticolumn{1}{x{3.833cm}}{\bf\textcolor{white}{rER in transport of proteins within pancreas cell}} \tn % Row 0 \SetRowColor{LightBackground} \mymulticolumn{1}{x{3.833cm}}{1. idea that ribosomes synthesise the \{ polypeptide / protein / eq \}} \tn % Row Count 2 (+ 2) % Row 1 \SetRowColor{white} \mymulticolumn{1}{x{3.833cm}}{2. proteins \{ move into / transported through / eq \} (the rER)} \tn % Row Count 4 (+ 2) % Row 2 \SetRowColor{LightBackground} \mymulticolumn{1}{x{3.833cm}}{3. protein is folded / forms \{3-D shape / secondary structure / tertiary structure \}} \tn % Row Count 6 (+ 2) % Row 3 \SetRowColor{white} \mymulticolumn{1}{x{3.833cm}}{4. idea that vesicles (containing the protein) are formed by rER} \tn % Row Count 8 (+ 2) \hhline{>{\arrayrulecolor{DarkBackground}}-} \end{tabularx} \par\addvspace{1.3em} \begin{tabularx}{3.833cm}{X} \SetRowColor{DarkBackground} \mymulticolumn{1}{x{3.833cm}}{\bf\textcolor{white}{How insulin is MPS by cell}} \tn % Row 0 \SetRowColor{LightBackground} \mymulticolumn{1}{x{3.833cm}}{1. in the rER insulin is folded e.g. forms \{3-D shape, secondary / tertiary structure} \tn % Row Count 2 (+ 2) % Row 1 \SetRowColor{white} \mymulticolumn{1}{x{3.833cm}}{2. insulin being packaged into (transport) vesicles by the rER ;} \tn % Row Count 4 (+ 2) % Row 2 \SetRowColor{LightBackground} \mymulticolumn{1}{x{3.833cm}}{3. vesicles \{ move to / fuse with / eq \} the Golgi apparatus / vesicles (fuse to) form the Golgi apparatus ;} \tn % Row Count 7 (+ 3) % Row 3 \SetRowColor{white} \mymulticolumn{1}{x{3.833cm}}{4. insulin being changed in Golgi apparatus ;} \tn % Row Count 8 (+ 1) % Row 4 \SetRowColor{LightBackground} \mymulticolumn{1}{x{3.833cm}}{5. idea of insulin being transferred in (secretory) vesicles from the Golgi apparatus to the cell (surface) membrane ;} \tn % Row Count 11 (+ 3) % Row 5 \SetRowColor{white} \mymulticolumn{1}{x{3.833cm}}{6. vesicles (containing insulin) fuse with cell (surface) membrane / exocytosis ;} \tn % Row Count 13 (+ 2) \hhline{>{\arrayrulecolor{DarkBackground}}-} \SetRowColor{LightBackground} \mymulticolumn{1}{x{3.833cm}}{{\bf{Note: MPS (abbreviation since I couldn't fit it in the title): modified, packaged and secreted}}} \tn \hhline{>{\arrayrulecolor{DarkBackground}}-} \end{tabularx} \par\addvspace{1.3em} \begin{tabularx}{3.833cm}{X} \SetRowColor{DarkBackground} \mymulticolumn{1}{x{3.833cm}}{\bf\textcolor{white}{Journey of protein in cell}} \tn % Row 0 \SetRowColor{LightBackground} \mymulticolumn{1}{x{3.833cm}}{1. proteins are produced on the ribosomes} \tn % Row Count 1 (+ 1) % Row 1 \SetRowColor{white} \mymulticolumn{1}{x{3.833cm}}{2. proteins which are produced on the ribosomes on the surface of rER are foded and processed in rER} \tn % Row Count 3 (+ 2) % Row 2 \SetRowColor{LightBackground} \mymulticolumn{1}{x{3.833cm}}{3. proteins are then modified in the golgi apparatus/body} \tn % Row Count 5 (+ 2) % Row 3 \SetRowColor{white} \mymulticolumn{1}{x{3.833cm}}{4. golgi apparatus packages proteins into vesicles around the cell} \tn % Row Count 7 (+ 2) % Row 4 \SetRowColor{LightBackground} \mymulticolumn{1}{x{3.833cm}}{5. proteins leave the cell by exocytosis once vesicles fuse with the cell membrane} \tn % Row Count 9 (+ 2) \hhline{>{\arrayrulecolor{DarkBackground}}-} \end{tabularx} \par\addvspace{1.3em} \begin{tabularx}{3.833cm}{X} \SetRowColor{DarkBackground} \mymulticolumn{1}{x{3.833cm}}{\bf\textcolor{white}{Subheading 3}} \tn % Row 0 \SetRowColor{LightBackground} \mymulticolumn{1}{x{3.833cm}}{{\bf{CELLS AND REPRODUCTION}}} \tn % Row Count 1 (+ 1) \hhline{>{\arrayrulecolor{DarkBackground}}-} \end{tabularx} \par\addvspace{1.3em} \begin{tabularx}{3.833cm}{X} \SetRowColor{DarkBackground} \mymulticolumn{1}{x{3.833cm}}{\bf\textcolor{white}{Definition of a locus}} \tn % Row 0 \SetRowColor{LightBackground} \mymulticolumn{1}{x{3.833cm}}{The location of a gene on a chromsome} \tn % Row Count 1 (+ 1) \hhline{>{\arrayrulecolor{DarkBackground}}-} \end{tabularx} \par\addvspace{1.3em} \begin{tabularx}{3.833cm}{X} \SetRowColor{DarkBackground} \mymulticolumn{1}{x{3.833cm}}{\bf\textcolor{white}{Definition of a sex-linked disorder}} \tn % Row 0 \SetRowColor{LightBackground} \mymulticolumn{1}{x{3.833cm}}{A disorder caused by a \{ mutated / faulty \} gene located on the \{X / Y \} chromsome} \tn % Row Count 2 (+ 2) \hhline{>{\arrayrulecolor{DarkBackground}}-} \end{tabularx} \par\addvspace{1.3em} \begin{tabularx}{3.833cm}{X} \SetRowColor{DarkBackground} \mymulticolumn{1}{x{3.833cm}}{\bf\textcolor{white}{Role of cell cycle}} \tn % Row 0 \SetRowColor{LightBackground} \mymulticolumn{1}{x{3.833cm}}{• growth of organism} \tn % Row Count 1 (+ 1) % Row 1 \SetRowColor{white} \mymulticolumn{1}{x{3.833cm}}{• asexual reproduction} \tn % Row Count 2 (+ 1) % Row 2 \SetRowColor{LightBackground} \mymulticolumn{1}{x{3.833cm}}{• repair of tissues} \tn % Row Count 3 (+ 1) % Row 3 \SetRowColor{white} \mymulticolumn{1}{x{3.833cm}}{• idea of control of cell \{ growth / division / mitosis \}} \tn % Row Count 5 (+ 2) \hhline{>{\arrayrulecolor{DarkBackground}}-} \end{tabularx} \par\addvspace{1.3em} \begin{tabularx}{3.833cm}{X} \SetRowColor{DarkBackground} \mymulticolumn{1}{x{3.833cm}}{\bf\textcolor{white}{Events of fertilisation after acrosome reaction}} \tn % Row 0 \SetRowColor{LightBackground} \mymulticolumn{1}{x{3.833cm}}{1. Fusion of sperm cell (membrane) with egg cell membrane} \tn % Row Count 2 (+ 2) % Row 1 \SetRowColor{white} \mymulticolumn{1}{x{3.833cm}}{2. Cortical granules release contents (into zona pellucida)} \tn % Row Count 4 (+ 2) % Row 2 \SetRowColor{LightBackground} \mymulticolumn{1}{x{3.833cm}}{3. Contents of cortical granules react with the zona pellucida / zona pellucida \{thickens / hardens \}} \tn % Row Count 7 (+ 3) % Row 3 \SetRowColor{white} \mymulticolumn{1}{x{3.833cm}}{4. Fusion of \{ sperm and egg / haploid \} nuclei} \tn % Row Count 8 (+ 1) \hhline{>{\arrayrulecolor{DarkBackground}}-} \end{tabularx} \par\addvspace{1.3em} \begin{tabularx}{3.833cm}{X} \SetRowColor{DarkBackground} \mymulticolumn{1}{x{3.833cm}}{\bf\textcolor{white}{Role of meiosis in production of gametes}} \tn % Row 0 \SetRowColor{LightBackground} \mymulticolumn{1}{x{3.833cm}}{1. idea of producing haploid \{ nuclei / cells \}} \tn % Row Count 1 (+ 1) % Row 1 \SetRowColor{white} \mymulticolumn{1}{x{3.833cm}}{2. genetic variation through \{ crossing over / independent assortment \} / eq} \tn % Row Count 3 (+ 2) % Row 2 \SetRowColor{LightBackground} \mymulticolumn{1}{x{3.833cm}}{3. Random / independent assortment gives rise to \{ new / different / eq \} combinations of (paternal and maternal) chromosomes} \tn % Row Count 6 (+ 3) % Row 3 \SetRowColor{white} \mymulticolumn{1}{x{3.833cm}}{4. crossing over involves swapping of \{ sections / eq \} of chromatids} \tn % Row Count 8 (+ 2) % Row 4 \SetRowColor{LightBackground} \mymulticolumn{1}{x{3.833cm}}{5. new combinations of alleles produced} \tn % Row Count 9 (+ 1) \hhline{>{\arrayrulecolor{DarkBackground}}-} \end{tabularx} \par\addvspace{1.3em} \begin{tabularx}{3.833cm}{x{1.7165 cm} x{1.7165 cm} } \SetRowColor{DarkBackground} \mymulticolumn{2}{x{3.833cm}}{\bf\textcolor{white}{Mitosis and meiosis comparison}} \tn % Row 0 \SetRowColor{LightBackground} \mymulticolumn{2}{x{3.833cm}}{• both mitosis and meiosis increase the number of cells} \tn % Row Count 2 (+ 2) % Row 1 \SetRowColor{white} • mitosis produces diploid cells & • meiosis produces haploid cells \tn % Row Count 4 (+ 2) % Row 2 \SetRowColor{LightBackground} • mitosis produces genetically identical cells & • meiosis produces cells that are genetically different to each other \tn % Row Count 8 (+ 4) % Row 3 \SetRowColor{white} • mitosis results in 8 spermatocytes from each stem cell & • meiosis results in 4 sperm cells from spermatocyte \tn % Row Count 11 (+ 3) % Row 4 \SetRowColor{LightBackground} • mitosis results in 2 genetically identical daughter cells & • meiosis results in 4 genetically varied daughter cells \tn % Row Count 15 (+ 4) \hhline{>{\arrayrulecolor{DarkBackground}}--} \end{tabularx} \par\addvspace{1.3em} \begin{tabularx}{3.833cm}{X} \SetRowColor{DarkBackground} \mymulticolumn{1}{x{3.833cm}}{\bf\textcolor{white}{How meiosis causes GV in gametes}} \tn % Row 0 \SetRowColor{LightBackground} \mymulticolumn{1}{x{3.833cm}}{1. independent assortment \{ of maternal and paternal chromosomes / eq \}} \tn % Row Count 2 (+ 2) % Row 1 \SetRowColor{white} \mymulticolumn{1}{x{3.833cm}}{2. crossing over - swapping over \{ DNA / sections of chromatid \} / eq} \tn % Row Count 4 (+ 2) % Row 2 \SetRowColor{LightBackground} \mymulticolumn{1}{x{3.833cm}}{3. produces recombinants / new combinations of alleles / eq} \tn % Row Count 6 (+ 2) \hhline{>{\arrayrulecolor{DarkBackground}}-} \end{tabularx} \par\addvspace{1.3em} \begin{tabularx}{3.833cm}{X} \SetRowColor{DarkBackground} \mymulticolumn{1}{x{3.833cm}}{\bf\textcolor{white}{Interphase (cell cycle)}} \tn % Row 0 \SetRowColor{LightBackground} \mymulticolumn{1}{x{3.833cm}}{1. G1 - cell grows bigger and replicates its organelles. A high amount of protein synthesis is taking place in order to build new organelles.} \tn % Row Count 3 (+ 3) % Row 1 \SetRowColor{white} \mymulticolumn{1}{x{3.833cm}}{2. S - The cell replicates its DNA} \tn % Row Count 4 (+ 1) % Row 2 \SetRowColor{LightBackground} \mymulticolumn{1}{x{3.833cm}}{3. G2 - The cell keeps growing until all of the organelles have duplicated.} \tn % Row Count 6 (+ 2) \hhline{>{\arrayrulecolor{DarkBackground}}-} \end{tabularx} \par\addvspace{1.3em} \begin{tabularx}{3.833cm}{X} \SetRowColor{DarkBackground} \mymulticolumn{1}{x{3.833cm}}{\bf\textcolor{white}{Mitosis stages}} \tn % Row 0 \SetRowColor{LightBackground} \mymulticolumn{1}{x{3.833cm}}{• Interphase – DNA rep / normal cell functions} \tn % Row Count 1 (+ 1) % Row 1 \SetRowColor{white} \mymulticolumn{1}{x{3.833cm}}{• Prophase – nuclear membrane breaks down / chromosomes condense become visible} \tn % Row Count 3 (+ 2) % Row 2 \SetRowColor{LightBackground} \mymulticolumn{1}{x{3.833cm}}{• Metaphase – meet in middle / equator} \tn % Row Count 4 (+ 1) % Row 3 \SetRowColor{white} \mymulticolumn{1}{x{3.833cm}}{• Anaphase – chromatids pulled to opposite poles} \tn % Row Count 6 (+ 2) % Row 4 \SetRowColor{LightBackground} \mymulticolumn{1}{x{3.833cm}}{• Telophase – nuclear membrane reformed – chromosomes lengthen} \tn % Row Count 8 (+ 2) \hhline{>{\arrayrulecolor{DarkBackground}}-} \end{tabularx} \par\addvspace{1.3em} \begin{tabularx}{3.833cm}{X} \SetRowColor{DarkBackground} \mymulticolumn{1}{x{3.833cm}}{\bf\textcolor{white}{CO and IA}} \tn % Row 0 \SetRowColor{LightBackground} \mymulticolumn{1}{x{3.833cm}}{• crossing over is the exchange of sections of DNA between non-sister chromatids} \tn % Row Count 2 (+ 2) % Row 1 \SetRowColor{white} \mymulticolumn{1}{x{3.833cm}}{• independent assortment is a random process where either chromosome from any gamete could be anywhere} \tn % Row Count 5 (+ 3) \hhline{>{\arrayrulecolor{DarkBackground}}-} \end{tabularx} \par\addvspace{1.3em} \begin{tabularx}{3.833cm}{X} \SetRowColor{DarkBackground} \mymulticolumn{1}{x{3.833cm}}{\bf\textcolor{white}{Subheading 4}} \tn % Row 0 \SetRowColor{LightBackground} \mymulticolumn{1}{x{3.833cm}}{{\bf{CORE PRACTICAL 5}}} \tn % Row Count 1 (+ 1) \hhline{>{\arrayrulecolor{DarkBackground}}-} \end{tabularx} \par\addvspace{1.3em} \begin{tabularx}{3.833cm}{X} \SetRowColor{DarkBackground} \mymulticolumn{1}{x{3.833cm}}{\bf\textcolor{white}{Controls for CP 5}} \tn % Row 0 \SetRowColor{LightBackground} \mymulticolumn{1}{x{3.833cm}}{• same variety/age/length of the garlic root tip} \tn % Row Count 1 (+ 1) % Row 1 \SetRowColor{white} \mymulticolumn{1}{x{3.833cm}}{• same stain (toluidine blue stain)} \tn % Row Count 2 (+ 1) % Row 2 \SetRowColor{LightBackground} \mymulticolumn{1}{x{3.833cm}}{• temperature} \tn % Row Count 3 (+ 1) % Row 3 \SetRowColor{white} \mymulticolumn{1}{x{3.833cm}}{• humidity} \tn % Row Count 4 (+ 1) % Row 4 \SetRowColor{LightBackground} \mymulticolumn{1}{x{3.833cm}}{• mineral ion concentration} \tn % Row Count 5 (+ 1) \hhline{>{\arrayrulecolor{DarkBackground}}-} \end{tabularx} \par\addvspace{1.3em} \begin{tabularx}{3.833cm}{X} \SetRowColor{DarkBackground} \mymulticolumn{1}{x{3.833cm}}{\bf\textcolor{white}{Stages of mitosis pract.}} \tn % Row 0 \SetRowColor{LightBackground} \mymulticolumn{1}{x{3.833cm}}{1. carefully remove the root tip from an onion that has been grown in water} \tn % Row Count 2 (+ 2) % Row 1 \SetRowColor{white} \mymulticolumn{1}{x{3.833cm}}{2. place the root tip in hydrochloric acid which will soften the tissue} \tn % Row Count 4 (+ 2) % Row 2 \SetRowColor{LightBackground} \mymulticolumn{1}{x{3.833cm}}{3. leave the root tip in the hydrochloric acid for 5 minutes then take it out and place it in distilled water} \tn % Row Count 7 (+ 3) % Row 3 \SetRowColor{white} \mymulticolumn{1}{x{3.833cm}}{4. add a few drops of toluidine blue stain} \tn % Row Count 8 (+ 1) % Row 4 \SetRowColor{LightBackground} \mymulticolumn{1}{x{3.833cm}}{5. add a cover slip on top and macerate the root tip} \tn % Row Count 10 (+ 2) % Row 5 \SetRowColor{white} \mymulticolumn{1}{x{3.833cm}}{6. view under a microscope from the lowest magnification first then the highest magnification} \tn % Row Count 12 (+ 2) % Row 6 \SetRowColor{LightBackground} \mymulticolumn{1}{x{3.833cm}}{7. calculate the mitotic index (insert equation here)} \tn % Row Count 14 (+ 2) \hhline{>{\arrayrulecolor{DarkBackground}}-} \end{tabularx} \par\addvspace{1.3em} \begin{tabularx}{3.833cm}{X} \SetRowColor{DarkBackground} \mymulticolumn{1}{x{3.833cm}}{\bf\textcolor{white}{How to prepare a RTS so chromosomes can be seen}} \tn % Row 0 \SetRowColor{LightBackground} \mymulticolumn{1}{x{3.833cm}}{1. removal of 5-10mm of root tips} \tn % Row Count 1 (+ 1) % Row 1 \SetRowColor{white} \mymulticolumn{1}{x{3.833cm}}{2. use of hydrochloric acid to separate cells / soften tissue} \tn % Row Count 3 (+ 2) % Row 2 \SetRowColor{LightBackground} \mymulticolumn{1}{x{3.833cm}}{3. add stain, e.g. toluidine (blue), orcein ;} \tn % Row Count 4 (+ 1) % Row 3 \SetRowColor{white} \mymulticolumn{1}{x{3.833cm}}{4. heating slide to intensify the colour / stain ;} \tn % Row Count 6 (+ 2) % Row 4 \SetRowColor{LightBackground} \mymulticolumn{1}{x{3.833cm}}{5. place the root tip on microscope slide, covering and squashing (to separate the cells)} \tn % Row Count 8 (+ 2) \hhline{>{\arrayrulecolor{DarkBackground}}-} \end{tabularx} \par\addvspace{1.3em} \begin{tabularx}{3.833cm}{X} \SetRowColor{DarkBackground} \mymulticolumn{1}{x{3.833cm}}{\bf\textcolor{white}{Mitosis practical (another ans.)}} \tn % Row 0 \SetRowColor{LightBackground} \mymulticolumn{1}{x{3.833cm}}{1. samples from different distances from the tip of the root taken} \tn % Row Count 2 (+ 2) % Row 1 \SetRowColor{white} \mymulticolumn{1}{x{3.833cm}}{2. measure distance from tip using an eyepiece graticule} \tn % Row Count 4 (+ 2) % Row 2 \SetRowColor{LightBackground} \mymulticolumn{1}{x{3.833cm}}{3. details of root tip squash procedure (e.g. correct use of hydrochloric acid, maceration procedure, squashing to produce single layer of cells)} \tn % Row Count 7 (+ 3) % Row 3 \SetRowColor{white} \mymulticolumn{1}{x{3.833cm}}{4. use of an appropriate named stain (e.g. toluidine stain, ethanoic orcein stain)} \tn % Row Count 9 (+ 2) % Row 4 \SetRowColor{LightBackground} \mymulticolumn{1}{x{3.833cm}}{5. squash \{ under a coverslip / on a microscope slide \}} \tn % Row Count 11 (+ 2) % Row 5 \SetRowColor{white} \mymulticolumn{1}{x{3.833cm}}{6. details of how to assess percentage of cells undergoing mitosis (e.g. count total number of cells and number of mitotic cells)} \tn % Row Count 14 (+ 3) % Row 6 \SetRowColor{LightBackground} \mymulticolumn{1}{x{3.833cm}}{{\bf{Note: Obviously you need to develop your answer. These are just points...}}} \tn % Row Count 16 (+ 2) \hhline{>{\arrayrulecolor{DarkBackground}}-} \end{tabularx} \par\addvspace{1.3em} \begin{tabularx}{3.833cm}{X} \SetRowColor{DarkBackground} \mymulticolumn{1}{x{3.833cm}}{\bf\textcolor{white}{Mitotic index}} \tn \SetRowColor{LightBackground} \mymulticolumn{1}{p{3.833cm}}{\vspace{1px}\centerline{\includegraphics[width=5.1cm]{/web/www.cheatography.com/public/uploads/hqneyroses_1702631892_mitotic+index.jpg}}} \tn \hhline{>{\arrayrulecolor{DarkBackground}}-} \end{tabularx} \par\addvspace{1.3em} % That's all folks \end{multicols*} \end{document}