\documentclass[10pt,a4paper]{article} % Packages \usepackage{fancyhdr} % For header and footer \usepackage{multicol} % Allows multicols in tables \usepackage{tabularx} % Intelligent column widths \usepackage{tabulary} % Used in header and footer \usepackage{hhline} % Border under tables \usepackage{graphicx} % For images \usepackage{xcolor} % For hex colours %\usepackage[utf8x]{inputenc} % For unicode character support \usepackage[T1]{fontenc} % Without this we get weird character replacements \usepackage{colortbl} % For coloured tables \usepackage{setspace} % For line height \usepackage{lastpage} % Needed for total page number \usepackage{seqsplit} % Splits long words. %\usepackage{opensans} % Can't make this work so far. Shame. Would be lovely. \usepackage[normalem]{ulem} % For underlining links % Most of the following are not required for the majority % of cheat sheets but are needed for some symbol support. \usepackage{amsmath} % Symbols \usepackage{MnSymbol} % Symbols \usepackage{wasysym} % Symbols %\usepackage[english,german,french,spanish,italian]{babel} % Languages % Document Info \author{fklimowski} \pdfinfo{ /Title (unit-2-prokaryotic-cells.pdf) /Creator (Cheatography) /Author (fklimowski) /Subject (UNIT 2: Prokaryotic Cells Cheat Sheet) } % Lengths and widths \addtolength{\textwidth}{6cm} \addtolength{\textheight}{-1cm} \addtolength{\hoffset}{-3cm} \addtolength{\voffset}{-2cm} \setlength{\tabcolsep}{0.2cm} % Space between columns \setlength{\headsep}{-12pt} % Reduce space between header and content \setlength{\headheight}{85pt} % If less, LaTeX automatically increases it \renewcommand{\footrulewidth}{0pt} % Remove footer line \renewcommand{\headrulewidth}{0pt} % Remove header line \renewcommand{\seqinsert}{\ifmmode\allowbreak\else\-\fi} % Hyphens in seqsplit % This two commands together give roughly % the right line height in the tables \renewcommand{\arraystretch}{1.3} \onehalfspacing % Commands \newcommand{\SetRowColor}[1]{\noalign{\gdef\RowColorName{#1}}\rowcolor{\RowColorName}} % Shortcut for row colour \newcommand{\mymulticolumn}[3]{\multicolumn{#1}{>{\columncolor{\RowColorName}}#2}{#3}} % For coloured multi-cols \newcolumntype{x}[1]{>{\raggedright}p{#1}} % New column types for ragged-right paragraph columns \newcommand{\tn}{\tabularnewline} % Required as custom column type in use % Font and Colours \definecolor{HeadBackground}{HTML}{333333} \definecolor{FootBackground}{HTML}{666666} \definecolor{TextColor}{HTML}{333333} \definecolor{DarkBackground}{HTML}{2786A3} \definecolor{LightBackground}{HTML}{F1F7F9} \renewcommand{\familydefault}{\sfdefault} \color{TextColor} % Header and Footer \pagestyle{fancy} \fancyhead{} % Set header to blank \fancyfoot{} % Set footer to blank \fancyhead[L]{ \noindent \begin{multicols}{3} \begin{tabulary}{5.8cm}{C} \SetRowColor{DarkBackground} \vspace{-7pt} {\parbox{\dimexpr\textwidth-2\fboxsep\relax}{\noindent \hspace*{-6pt}\includegraphics[width=5.8cm]{/web/www.cheatography.com/public/images/cheatography_logo.pdf}} } \end{tabulary} \columnbreak \begin{tabulary}{11cm}{L} \vspace{-2pt}\large{\bf{\textcolor{DarkBackground}{\textrm{UNIT 2: Prokaryotic Cells Cheat Sheet}}}} \\ \normalsize{by \textcolor{DarkBackground}{fklimowski} via \textcolor{DarkBackground}{\uline{cheatography.com/172179/cs/36197/}}} \end{tabulary} \end{multicols}} \fancyfoot[L]{ \footnotesize \noindent \begin{multicols}{3} \begin{tabulary}{5.8cm}{LL} \SetRowColor{FootBackground} \mymulticolumn{2}{p{5.377cm}}{\bf\textcolor{white}{Cheatographer}} \\ \vspace{-2pt}fklimowski \\ \uline{cheatography.com/fklimowski} \\ \end{tabulary} \vfill \columnbreak \begin{tabulary}{5.8cm}{L} \SetRowColor{FootBackground} \mymulticolumn{1}{p{5.377cm}}{\bf\textcolor{white}{Cheat Sheet}} \\ \vspace{-2pt}Published 20th December, 2022.\\ Updated 20th December, 2022.\\ Page {\thepage} of \pageref{LastPage}. \end{tabulary} \vfill \columnbreak \begin{tabulary}{5.8cm}{L} \SetRowColor{FootBackground} \mymulticolumn{1}{p{5.377cm}}{\bf\textcolor{white}{Sponsor}} \\ \SetRowColor{white} \vspace{-5pt} %\includegraphics[width=48px,height=48px]{dave.jpeg} Measure your website readability!\\ www.readability-score.com \end{tabulary} \end{multicols}} \begin{document} \raggedright \raggedcolumns % Set font size to small. Switch to any value % from this page to resize cheat sheet text: % www.emerson.emory.edu/services/latex/latex_169.html \footnotesize % Small font. \begin{multicols*}{3} \begin{tabularx}{5.377cm}{x{1.64772 cm} x{1.73926 cm} x{1.19002 cm} } \SetRowColor{DarkBackground} \mymulticolumn{3}{x{5.377cm}}{\bf\textcolor{white}{prokaryotic vs eukaryotic cells}} \tn % Row 0 \SetRowColor{LightBackground} PROKARYOTE & EUKARYOTE & BOTH \tn % Row Count 1 (+ 1) % Row 1 \SetRowColor{white} Tiny (≈ 0.2 - 10 μm) & Bigger (≈ 10 - 100 μm) & Ribosomes \tn % Row Count 3 (+ 2) % Row 2 \SetRowColor{LightBackground} No membrane bound organelles & Has membrane bound organelles & DNA \tn % Row Count 5 (+ 2) % Row 3 \SetRowColor{white} Division by binary fission & Division by binary fission, mitosis or meiosis & Cell membrane \tn % Row Count 9 (+ 4) % Row 4 \SetRowColor{LightBackground} Cell wall with peptidoglycan & Cell wall with cellulose (plants) or chitin (fungus) & Cytoplasm \tn % Row Count 13 (+ 4) % Row 5 \SetRowColor{white} DNA in nucleoid (no nuclear membrane) & DNA in nucleus (with nuclear membrane) & Essential functions of life \tn % Row Count 16 (+ 3) % Row 6 \SetRowColor{LightBackground} Flagella rotates & Flagella moves laterally & \tn % Row Count 18 (+ 2) % Row 7 \SetRowColor{white} DNA is circular and naked & DNA is linear and associated with histone proteins & \tn % Row Count 22 (+ 4) % Row 8 \SetRowColor{LightBackground} Smaller 70s ribosomes & Larger 80s ribosomes & \tn % Row Count 24 (+ 2) % Row 9 \SetRowColor{white} Can have plasmids & Does not have plasmids & \tn % Row Count 26 (+ 2) \hhline{>{\arrayrulecolor{DarkBackground}}---} \end{tabularx} \par\addvspace{1.3em} \begin{tabularx}{5.377cm}{X} \SetRowColor{DarkBackground} \mymulticolumn{1}{x{5.377cm}}{\bf\textcolor{white}{Prokaryotic DNA}} \tn % Row 0 \SetRowColor{LightBackground} \mymulticolumn{1}{x{5.377cm}}{Prokaryotic cell DNA can be found in two locations:} \tn % Row Count 2 (+ 2) % Row 1 \SetRowColor{white} \mymulticolumn{1}{x{5.377cm}}{Nucleoid: main DNA of the cell. DNA is not enclosed in a membrane (found freely cytoplasm) DNA is a single loop DNA is not wrapped around proteins (termed "naked")} \tn % Row Count 6 (+ 4) % Row 2 \SetRowColor{LightBackground} \mymulticolumn{1}{x{5.377cm}}{Plasmid: extra piece(s) of DNA Circular and naked Smaller than main nucleoid DNA Replicates independently of the nucleoid DNA Not found in all prokaryotic cells Can be shared between bacteria Often contain genes for antibiotic resistance} \tn % Row Count 11 (+ 5) % Row 3 \SetRowColor{white} \mymulticolumn{1}{x{5.377cm}}{Naked means that the DNA is not associated with proteins.} \tn % Row Count 13 (+ 2) % Row 4 \SetRowColor{LightBackground} \mymulticolumn{1}{x{5.377cm}}{Extracellular means outside of cell} \tn % Row Count 14 (+ 1) \hhline{>{\arrayrulecolor{DarkBackground}}-} \end{tabularx} \par\addvspace{1.3em} \begin{tabularx}{5.377cm}{X} \SetRowColor{DarkBackground} \mymulticolumn{1}{x{5.377cm}}{\bf\textcolor{white}{Antibiotics}} \tn % Row 0 \SetRowColor{LightBackground} \mymulticolumn{1}{x{5.377cm}}{Antibiotics are chemicals that kill bacteria or inhibit bacteria replication.} \tn % Row Count 2 (+ 2) % Row 1 \SetRowColor{white} \mymulticolumn{1}{x{5.377cm}}{Antibiotics block processes that occur in prokaryotic cells but not in eukaryotic cells.} \tn % Row Count 4 (+ 2) % Row 2 \SetRowColor{LightBackground} \mymulticolumn{1}{x{5.377cm}}{Some antibiotics damage prokaryotic DNA} \tn % Row Count 5 (+ 1) % Row 3 \SetRowColor{white} \mymulticolumn{1}{x{5.377cm}}{Some antibiotics block prokaryotic ribosomes (70S)} \tn % Row Count 6 (+ 1) % Row 4 \SetRowColor{LightBackground} \mymulticolumn{1}{x{5.377cm}}{Some antibiotics block bacterial cell wall synthesis} \tn % Row Count 8 (+ 2) % Row 5 \SetRowColor{white} \mymulticolumn{1}{x{5.377cm}}{Some antibiotics block bacterial metabolism} \tn % Row Count 9 (+ 1) % Row 6 \SetRowColor{LightBackground} \mymulticolumn{1}{x{5.377cm}}{Antibiotics block specific structures and metabolic pathways found in bacteria. Viruses do not have these structures or metabolic pathways. No cell wall No ribosomes or protein synthesis Do not replicate their own DNA (hijack the host cell replication machinery)} \tn % Row Count 15 (+ 6) \hhline{>{\arrayrulecolor{DarkBackground}}-} \end{tabularx} \par\addvspace{1.3em} \begin{tabularx}{5.377cm}{X} \SetRowColor{DarkBackground} \mymulticolumn{1}{x{5.377cm}}{\bf\textcolor{white}{Functions within Prokaryote}} \tn % Row 0 \SetRowColor{LightBackground} \mymulticolumn{1}{x{5.377cm}}{Cell (plasma) membrane: responsible for regulating what materials move into and out of the cell} \tn % Row Count 2 (+ 2) % Row 1 \SetRowColor{white} \mymulticolumn{1}{x{5.377cm}}{Nucleoid: DNA with ends that come together to form a circle and is NOT wrapped around proteins (termed "naked")} \tn % Row Count 5 (+ 3) % Row 2 \SetRowColor{LightBackground} \mymulticolumn{1}{x{5.377cm}}{Cytoplasm (cytosol): gel-like fluid substance (mostly water with many dissolved molecules), site of metabolic reactions} \tn % Row Count 8 (+ 3) % Row 3 \SetRowColor{white} \mymulticolumn{1}{x{5.377cm}}{Ribosome: build proteins during translation} \tn % Row Count 9 (+ 1) % Row 4 \SetRowColor{LightBackground} \mymulticolumn{1}{x{5.377cm}}{Plasmid*: extra piece(s) of small, circular DNA that can be shared between bacteria, often contain genes for antibiotic resistance} \tn % Row Count 12 (+ 3) % Row 5 \SetRowColor{white} \mymulticolumn{1}{x{5.377cm}}{Cell wall*: provides shape and allows the cell to withstand turgor pressure without bursting} \tn % Row Count 14 (+ 2) % Row 6 \SetRowColor{LightBackground} \mymulticolumn{1}{x{5.377cm}}{Pili*: enable the cell to attach to surfaces} \tn % Row Count 15 (+ 1) % Row 7 \SetRowColor{white} \mymulticolumn{1}{x{5.377cm}}{Capsule*: helps the cell keep from dehydrating and adhere to surfaces} \tn % Row Count 17 (+ 2) % Row 8 \SetRowColor{LightBackground} \mymulticolumn{1}{x{5.377cm}}{Flagellum*: Long extensions used in cell locomotion (movement)} \tn % Row Count 19 (+ 2) % Row 9 \SetRowColor{white} \mymulticolumn{1}{x{5.377cm}}{* Structure is found in SOME but not ALL prokaryotic cells.} \tn % Row Count 21 (+ 2) \hhline{>{\arrayrulecolor{DarkBackground}}-} \end{tabularx} \par\addvspace{1.3em} \begin{tabularx}{5.377cm}{X} \SetRowColor{DarkBackground} \mymulticolumn{1}{x{5.377cm}}{\bf\textcolor{white}{Genetic Material}} \tn \SetRowColor{LightBackground} \mymulticolumn{1}{p{5.377cm}}{\vspace{1px}\centerline{\includegraphics[width=5.1cm]{/web/www.cheatography.com/public/uploads/fklimowski_1671538989_Screenshot 2022-12-20 at 5.52.25 PM.png}}} \tn \hhline{>{\arrayrulecolor{DarkBackground}}-} \end{tabularx} \par\addvspace{1.3em} \begin{tabularx}{5.377cm}{X} \SetRowColor{DarkBackground} \mymulticolumn{1}{x{5.377cm}}{\bf\textcolor{white}{Binary Fission}} \tn % Row 0 \SetRowColor{LightBackground} \mymulticolumn{1}{x{5.377cm}}{Reproduction is one of the functions of life.} \tn % Row Count 1 (+ 1) % Row 1 \SetRowColor{white} \mymulticolumn{1}{x{5.377cm}}{Asexual reproduction is a type of reproduction in which: Offspring arise from a single parent cell or organism The offspring are genetically identical to the parent} \tn % Row Count 5 (+ 4) % Row 2 \SetRowColor{LightBackground} \mymulticolumn{1}{x{5.377cm}}{Binary fission and mitosis are mechanisms of asexual reproduction.} \tn % Row Count 7 (+ 2) % Row 3 \SetRowColor{white} \mymulticolumn{1}{x{5.377cm}}{1. Prepare for Division The parent cell must have enough energy and resources in order to divide.} \tn % Row Count 9 (+ 2) % Row 4 \SetRowColor{LightBackground} \mymulticolumn{1}{x{5.377cm}}{2. DNA Replication The replication of the circular DNA is semi- conservative and depends on complementary base pairing. Plasmid replicates separately.} \tn % Row Count 13 (+ 4) % Row 5 \SetRowColor{white} \mymulticolumn{1}{x{5.377cm}}{3. DNA Attachment The two DNA molecules each attach to a different part of the cell membrane. Cell Growth The cell builds more membrane and wall, elongating the cell} \tn % Row Count 17 (+ 4) % Row 6 \SetRowColor{LightBackground} \mymulticolumn{1}{x{5.377cm}}{4. Pinching In The cell membrane and wall continue to grow and begin to pinch inwards, creating furrows in the cell edge.} \tn % Row Count 20 (+ 3) % Row 7 \SetRowColor{white} \mymulticolumn{1}{x{5.377cm}}{5. Division The cell membrane and wall continue to grow, with the two furrows eventually meeting (called a septum) to separate the cell into two.} \tn % Row Count 23 (+ 3) % Row 8 \SetRowColor{LightBackground} \mymulticolumn{1}{x{5.377cm}}{6. Daughter Cells The two daughter cells that result from binary fission are genetically identical to the single cell from which they arose.} \tn % Row Count 26 (+ 3) \hhline{>{\arrayrulecolor{DarkBackground}}-} \end{tabularx} \par\addvspace{1.3em} \begin{tabularx}{5.377cm}{X} \SetRowColor{DarkBackground} \mymulticolumn{1}{x{5.377cm}}{\bf\textcolor{white}{Ribosomes}} \tn % Row 0 \SetRowColor{LightBackground} \mymulticolumn{1}{x{5.377cm}}{In both prokaryotes and eukaryotes, ribosomes... catalyzes the synthesis (creation) of proteins are composed of two subunits that come together to form a functioning structure.} \tn % Row Count 4 (+ 4) % Row 1 \SetRowColor{white} \mymulticolumn{1}{x{5.377cm}}{Prokaryotes have smaller (70s) ribosomes.} \tn % Row Count 5 (+ 1) % Row 2 \SetRowColor{LightBackground} \mymulticolumn{1}{x{5.377cm}}{Eukaryotes have larger (80s) ribosomes.} \tn % Row Count 6 (+ 1) \hhline{>{\arrayrulecolor{DarkBackground}}-} \end{tabularx} \par\addvspace{1.3em} \begin{tabularx}{5.377cm}{X} \SetRowColor{DarkBackground} \mymulticolumn{1}{x{5.377cm}}{\bf\textcolor{white}{Micrographs}} \tn % Row 0 \SetRowColor{LightBackground} \mymulticolumn{1}{x{5.377cm}}{Ultrastructures are cellular structures that are too small to be seen with a light microscope. Since prokaryotic cells are so small, any detail requires an electron microscope to be seen.} \tn % Row Count 4 (+ 4) \hhline{>{\arrayrulecolor{DarkBackground}}-} \end{tabularx} \par\addvspace{1.3em} \begin{tabularx}{5.377cm}{X} \SetRowColor{DarkBackground} \mymulticolumn{1}{x{5.377cm}}{\bf\textcolor{white}{E. Coli}} \tn \SetRowColor{LightBackground} \mymulticolumn{1}{p{5.377cm}}{\vspace{1px}\centerline{\includegraphics[width=5.1cm]{/web/www.cheatography.com/public/uploads/fklimowski_1671538821_Screenshot 2022-12-20 at 5.50.01 PM.png}}} \tn \hhline{>{\arrayrulecolor{DarkBackground}}-} \end{tabularx} \par\addvspace{1.3em} \begin{tabularx}{5.377cm}{X} \SetRowColor{DarkBackground} \mymulticolumn{1}{x{5.377cm}}{\bf\textcolor{white}{E. Coli}} \tn % Row 0 \SetRowColor{LightBackground} \mymulticolumn{1}{x{5.377cm}}{Drawing should include} \tn % Row Count 1 (+ 1) % Row 1 \SetRowColor{white} \mymulticolumn{1}{x{5.377cm}}{Cell wall – uniformly thick and drawn outside the plasma membrane; Capsule – drawn outside the cell wall; Plasma / cell membrane – drawn as a continuous single line; Cytoplasm; Ribosome – drawn as a small circle or dark dot; Nucleoid- shown as a tangle of thread or irregular shape without a nuclear membrane; Flagella – shown to be longer than any pili; Pili - hair like structures Plasmid – circular ring of DNA; Size stated -{}- 1 to 10 μm} \tn % Row Count 11 (+ 10) \hhline{>{\arrayrulecolor{DarkBackground}}-} \end{tabularx} \par\addvspace{1.3em} \begin{tabularx}{5.377cm}{X} \SetRowColor{DarkBackground} \mymulticolumn{1}{x{5.377cm}}{\bf\textcolor{white}{Florey and Chain}} \tn % Row 0 \SetRowColor{LightBackground} \mymulticolumn{1}{x{5.377cm}}{Florey and Chain were the scientists who followed up most successfully on Alexander Fleming's discovery of penicillin, sharing with him the 1945 Nobel Prize in Physiology or Medicine.} \tn % Row Count 4 (+ 4) % Row 1 \SetRowColor{white} \mymulticolumn{1}{x{5.377cm}}{Tested the antibacterial properties of penicillin on mice. 8 mice were injected with disease causing Streptococci (infectious bacteria) 4 of these were then injected with penicillin, the other 4 were not 16 hours later, the 4 mice that had received penicillin were alive, but the untreated mice were dead.} \tn % Row Count 11 (+ 7) \hhline{>{\arrayrulecolor{DarkBackground}}-} \end{tabularx} \par\addvspace{1.3em} \begin{tabularx}{5.377cm}{x{2.4885 cm} x{2.4885 cm} } \SetRowColor{DarkBackground} \mymulticolumn{2}{x{5.377cm}}{\bf\textcolor{white}{Research Risks}} \tn % Row 0 \SetRowColor{LightBackground} Then & Now \tn % Row Count 1 (+ 1) % Row 1 \SetRowColor{white} tested humans without consent, no protocols or rules to follow, impure drug sample, rushed, fast testing trial, patients at the point of death & testing humans not allowed without consent, strict protocols, highly purified drug samples, drug testing, healthy people often tested before they get sick \tn % Row Count 9 (+ 8) \hhline{>{\arrayrulecolor{DarkBackground}}--} \end{tabularx} \par\addvspace{1.3em} % That's all folks \end{multicols*} \end{document}