\documentclass[10pt,a4paper]{article} % Packages \usepackage{fancyhdr} % For header and footer \usepackage{multicol} % Allows multicols in tables \usepackage{tabularx} % Intelligent column widths \usepackage{tabulary} % Used in header and footer \usepackage{hhline} % Border under tables \usepackage{graphicx} % For images \usepackage{xcolor} % For hex colours %\usepackage[utf8x]{inputenc} % For unicode character support \usepackage[T1]{fontenc} % Without this we get weird character replacements \usepackage{colortbl} % For coloured tables \usepackage{setspace} % For line height \usepackage{lastpage} % Needed for total page number \usepackage{seqsplit} % Splits long words. %\usepackage{opensans} % Can't make this work so far. Shame. Would be lovely. \usepackage[normalem]{ulem} % For underlining links % Most of the following are not required for the majority % of cheat sheets but are needed for some symbol support. \usepackage{amsmath} % Symbols \usepackage{MnSymbol} % Symbols \usepackage{wasysym} % Symbols %\usepackage[english,german,french,spanish,italian]{babel} % Languages % Document Info \author{dolly} \pdfinfo{ /Title (the-evolution-of-microorganisms-and-microbiology.pdf) /Creator (Cheatography) /Author (dolly) /Subject (The Evolution of Microorganisms and Microbiology Cheat Sheet) } % Lengths and widths \addtolength{\textwidth}{6cm} \addtolength{\textheight}{-1cm} \addtolength{\hoffset}{-3cm} \addtolength{\voffset}{-2cm} \setlength{\tabcolsep}{0.2cm} % Space between columns \setlength{\headsep}{-12pt} % Reduce space between header and content \setlength{\headheight}{85pt} % If less, LaTeX automatically increases it \renewcommand{\footrulewidth}{0pt} % Remove footer line \renewcommand{\headrulewidth}{0pt} % Remove header line \renewcommand{\seqinsert}{\ifmmode\allowbreak\else\-\fi} % Hyphens in seqsplit % This two commands together give roughly % the right line height in the tables \renewcommand{\arraystretch}{1.3} \onehalfspacing % Commands \newcommand{\SetRowColor}[1]{\noalign{\gdef\RowColorName{#1}}\rowcolor{\RowColorName}} % Shortcut for row colour \newcommand{\mymulticolumn}[3]{\multicolumn{#1}{>{\columncolor{\RowColorName}}#2}{#3}} % For coloured multi-cols \newcolumntype{x}[1]{>{\raggedright}p{#1}} % New column types for ragged-right paragraph columns \newcommand{\tn}{\tabularnewline} % Required as custom column type in use % Font and Colours \definecolor{HeadBackground}{HTML}{333333} \definecolor{FootBackground}{HTML}{666666} \definecolor{TextColor}{HTML}{333333} \definecolor{DarkBackground}{HTML}{9C3267} \definecolor{LightBackground}{HTML}{F8F2F5} \renewcommand{\familydefault}{\sfdefault} \color{TextColor} % Header and Footer \pagestyle{fancy} \fancyhead{} % Set header to blank \fancyfoot{} % Set footer to blank \fancyhead[L]{ \noindent \begin{multicols}{3} \begin{tabulary}{5.8cm}{C} \SetRowColor{DarkBackground} \vspace{-7pt} {\parbox{\dimexpr\textwidth-2\fboxsep\relax}{\noindent \hspace*{-6pt}\includegraphics[width=5.8cm]{/web/www.cheatography.com/public/images/cheatography_logo.pdf}} } \end{tabulary} \columnbreak \begin{tabulary}{11cm}{L} \vspace{-2pt}\large{\bf{\textcolor{DarkBackground}{\textrm{The Evolution of Microorganisms and Microbiology Cheat Sheet}}}} \\ \normalsize{by \textcolor{DarkBackground}{dolly} via \textcolor{DarkBackground}{\uline{cheatography.com/183950/cs/38322/}}} \end{tabulary} \end{multicols}} \fancyfoot[L]{ \footnotesize \noindent \begin{multicols}{3} \begin{tabulary}{5.8cm}{LL} \SetRowColor{FootBackground} \mymulticolumn{2}{p{5.377cm}}{\bf\textcolor{white}{Cheatographer}} \\ \vspace{-2pt}dolly \\ \uline{cheatography.com/dolly} \\ \end{tabulary} \vfill \columnbreak \begin{tabulary}{5.8cm}{L} \SetRowColor{FootBackground} \mymulticolumn{1}{p{5.377cm}}{\bf\textcolor{white}{Cheat Sheet}} \\ \vspace{-2pt}Not Yet Published.\\ Updated 21st April, 2023.\\ Page {\thepage} of \pageref{LastPage}. \end{tabulary} \vfill \columnbreak \begin{tabulary}{5.8cm}{L} \SetRowColor{FootBackground} \mymulticolumn{1}{p{5.377cm}}{\bf\textcolor{white}{Sponsor}} \\ \SetRowColor{white} \vspace{-5pt} %\includegraphics[width=48px,height=48px]{dave.jpeg} Measure your website readability!\\ www.readability-score.com \end{tabulary} \end{multicols}} \begin{document} \raggedright \raggedcolumns % Set font size to small. Switch to any value % from this page to resize cheat sheet text: % www.emerson.emory.edu/services/latex/latex_169.html \footnotesize % Small font. \begin{multicols*}{2} \begin{tabularx}{8.4cm}{X} \SetRowColor{DarkBackground} \mymulticolumn{1}{x{8.4cm}}{\bf\textcolor{white}{The Importance of Microorganisms}} \tn % Row 0 \SetRowColor{LightBackground} \mymulticolumn{1}{x{8.4cm}}{Who are the members of the microbial world?} \tn \mymulticolumn{1}{x{8.4cm}}{\hspace*{6 px}\rule{2px}{6px}\hspace*{6 px}- Cellular and acellular microorganisms too small to be clearly seen by the unaided eye\{\{nl\}\}- Organisms with no highly differentiated tissues\{\{nl\}\}- Relatively simple in their constructions} \tn % Row Count 6 (+ 6) % Row 1 \SetRowColor{white} \mymulticolumn{1}{x{8.4cm}}{What are the types of microorganisms} \tn \mymulticolumn{1}{x{8.4cm}}{\hspace*{6 px}\rule{2px}{6px}\hspace*{6 px}1. Cellular - Includes Fungi, Protists, Bacteria and Archaea\{\{nl\}\}2. Acellular (Not made up of cells/divided into cells) - Includes Virus, Viroids, Satellites and Prions} \tn % Row Count 11 (+ 5) % Row 2 \SetRowColor{LightBackground} \mymulticolumn{1}{x{8.4cm}}{Describe prokaryotic cells} \tn \mymulticolumn{1}{x{8.4cm}}{\hspace*{6 px}\rule{2px}{6px}\hspace*{6 px}Their contents are not divided into compartments by membranes} \tn % Row Count 14 (+ 3) % Row 3 \SetRowColor{white} \mymulticolumn{1}{x{8.4cm}}{Describe eukaryotic cells} \tn \mymulticolumn{1}{x{8.4cm}}{\hspace*{6 px}\rule{2px}{6px}\hspace*{6 px}- Have a membrane-enclosed organelles\{\{nl\}\}- More complex morphologically\{\{nl\}\}- Usually larger than prokaryotes} \tn % Row Count 18 (+ 4) % Row 4 \SetRowColor{LightBackground} \mymulticolumn{1}{x{8.4cm}}{What are the three domain systems?} \tn \mymulticolumn{1}{x{8.4cm}}{\hspace*{6 px}\rule{2px}{6px}\hspace*{6 px}- Bacteria\{\{nl\}\}- Archaea\{\{nl\}\}- Eukaryotes} \tn % Row Count 20 (+ 2) % Row 5 \SetRowColor{white} \mymulticolumn{1}{x{8.4cm}}{Describe the domain bacteria} \tn \mymulticolumn{1}{x{8.4cm}}{\hspace*{6 px}\rule{2px}{6px}\hspace*{6 px}- Usually single-celled organism\{\{nl\}\} - Contain peptidoglycan in their cell wall\{\{nl\}\}- Most lack membrane bounded nucleus\{\{nl\}\}- Ubiquitous and some live in extreme environments} \tn % Row Count 25 (+ 5) % Row 6 \SetRowColor{LightBackground} \mymulticolumn{1}{x{8.4cm}}{Describe the domain archaea} \tn \mymulticolumn{1}{x{8.4cm}}{\hspace*{6 px}\rule{2px}{6px}\hspace*{6 px}- Distinguished from bacteria by unique rRNA sequences\{\{nl\}\}- Have unique membrane lipids\{\{nl\}\}- Lack peptidoglycan in their cell walls\{\{nl\}\}- Many live in extreme environments} \tn % Row Count 30 (+ 5) \end{tabularx} \par\addvspace{1.3em} \vfill \columnbreak \begin{tabularx}{8.4cm}{X} \SetRowColor{DarkBackground} \mymulticolumn{1}{x{8.4cm}}{\bf\textcolor{white}{The Importance of Microorganisms (cont)}} \tn % Row 7 \SetRowColor{white} \mymulticolumn{1}{x{8.4cm}}{Describe the domain eukarya} \tn \mymulticolumn{1}{x{8.4cm}}{\hspace*{6 px}\rule{2px}{6px}\hspace*{6 px}- Include plants, animals, protists and fungi\{\{nl\}\} - Protists are generally larger than bacteria and archaea\{\{nl\}\}- Fungi have metabolic capabilities} \tn % Row Count 5 (+ 5) % Row 8 \SetRowColor{LightBackground} \mymulticolumn{1}{x{8.4cm}}{What are the differences between organisms' rRNA?} \tn \mymulticolumn{1}{x{8.4cm}}{\hspace*{6 px}\rule{2px}{6px}\hspace*{6 px}- Archaea SSU rRNA are more similar to eukaryotes compared to bacteria\{\{nl\}\}- Prokaryotes have tRNA on their rRNA\{\{nl\}\}- Prokaryotes have longer rRNA compared to eukaryotes} \tn % Row Count 10 (+ 5) % Row 9 \SetRowColor{white} \mymulticolumn{1}{x{8.4cm}}{Describe viruses} \tn \mymulticolumn{1}{x{8.4cm}}{\hspace*{6 px}\rule{2px}{6px}\hspace*{6 px}- Smallest of all microbes\{\{nl\}\}- Require host cell to infect\{\{nl\}\}- Consist of nucleic acid and protein} \tn % Row Count 14 (+ 4) % Row 10 \SetRowColor{LightBackground} \mymulticolumn{1}{x{8.4cm}}{Describe viroids and satellites} \tn \mymulticolumn{1}{x{8.4cm}}{\hspace*{6 px}\rule{2px}{6px}\hspace*{6 px}Composed of RNA only but some have DNA} \tn % Row Count 16 (+ 2) % Row 11 \SetRowColor{white} \mymulticolumn{1}{x{8.4cm}}{Describe prions} \tn \mymulticolumn{1}{x{8.4cm}}{\hspace*{6 px}\rule{2px}{6px}\hspace*{6 px}- Infectious proteins\{\{nl\}\}- Lack nucleic acid} \tn % Row Count 18 (+ 2) % Row 12 \SetRowColor{LightBackground} \mymulticolumn{1}{x{8.4cm}}{How did the methods used to classify microbes changed?} \tn \mymulticolumn{1}{x{8.4cm}}{\hspace*{6 px}\rule{2px}{6px}\hspace*{6 px}Before\{\{nl\}\}- Organisms were classified into five kingdoms ( Monera, Protista, Fungi, Animalia, Plantae)\{\{nl\}\}- All organisms with prokaryotic cell structures are under Monera\{\{nl\}\}- Prokaryotes are too diverse to be grouped together in a single kingdom so this is invalid\{\{nl\}\}After\{\{nl\}\}- Recent discoveries on rRNA lead to classification into three domains (Bacteria, Archaea, Eukaryotes)} \tn % Row Count 29 (+ 11) % Row 13 \SetRowColor{white} \mymulticolumn{1}{x{8.4cm}}{Different characteristic that distinguish microorganisms from the other} \tn \mymulticolumn{1}{x{8.4cm}}{\hspace*{6 px}\rule{2px}{6px}\hspace*{6 px}- Bacteria: Contain peptidoglycan in their cell walls\{\{nl\}\}- Archaea: Have unique membrane lipid\{\{nl\}\}- Protists: Usually larger than bacteria and archaea\{\{nl\}\}- Fungi: Have metabolic capabilities\{\{nl\}\}- Viruses: Composed of nucleic acid and proteins\{\{nl\}\}- Viroids: Composed of RNA only\{\{nl\}\}- Satellites: Composed of RNA/DNA\{\{nl\}\}- Prions: Composed of infectious protein only} \tn % Row Count 40 (+ 11) \hhline{>{\arrayrulecolor{DarkBackground}}-} \end{tabularx} \par\addvspace{1.3em} \begin{tabularx}{8.4cm}{X} \SetRowColor{DarkBackground} \mymulticolumn{1}{x{8.4cm}}{\bf\textcolor{white}{Microbiology and Its Origins}} \tn % Row 0 \SetRowColor{LightBackground} \mymulticolumn{1}{x{8.4cm}}{Explain the endosymbiotic hypothesis} \tn \mymulticolumn{1}{x{8.4cm}}{\hspace*{6 px}\rule{2px}{6px}\hspace*{6 px}Over time the bacterial endosymbiont of ancestral cell in the eukaryotic lineage lost its ability to live independently, becoming either a mitochondrion if it used aerobic respirations or chloroplasts if it was a photosynthetic bacterium} \tn % Row Count 7 (+ 7) % Row 1 \SetRowColor{white} \mymulticolumn{1}{x{8.4cm}}{What are the evidences to support endosymbiotic hypothesis?} \tn \mymulticolumn{1}{x{8.4cm}}{\hspace*{6 px}\rule{2px}{6px}\hspace*{6 px}- Mitochondria and chloroplasts have similar SSU rRNA with bacteria:Mitochondria - proteobacteria while chloroplast and green algae - cyanobacterium\{\{nl\}\}- Peptidoglycan found in chloroplasts\{\{nl\}\}- Mitochondria and chloroplast have similar DNA and ribosomes with Bacteria's} \tn % Row Count 15 (+ 8) % Row 2 \SetRowColor{LightBackground} \mymulticolumn{1}{x{8.4cm}}{Explain the hyrogen hypothesis} \tn \mymulticolumn{1}{x{8.4cm}}{\hspace*{6 px}\rule{2px}{6px}\hspace*{6 px}- The endosymbiont was an anaerobic bacterium that produces hydrogen and carbon dioxide as end products of its metabolism\{\{nl\}\} -Over time, the host become dependent to the hydrogen produced by the endosymbiont thus it evolved into several organelles\{\{nl\}\}- Perform aerobic respiration: Mitochondria\{\{nl\}\} - Produce ATP through fermentation: Hydrogenosome} \tn % Row Count 24 (+ 9) % Row 3 \SetRowColor{white} \mymulticolumn{1}{x{8.4cm}}{Describe Koch's Postulates} \tn \mymulticolumn{1}{x{8.4cm}}{\hspace*{6 px}\rule{2px}{6px}\hspace*{6 px}1. The microorganism must be present in every case of the disease but absent from healthy organisms\{\{nl\}\}2. The suspected microorganism must be isolated and grown in a pure culture\{\{nl\}\}3. The same disease must result when the microorganism is inoculated into a healthy host\{\{nl\}\}4. The same microorganism must be isolated from the infected host} \tn % Row Count 33 (+ 9) \end{tabularx} \par\addvspace{1.3em} \vfill \columnbreak \begin{tabularx}{8.4cm}{X} \SetRowColor{DarkBackground} \mymulticolumn{1}{x{8.4cm}}{\bf\textcolor{white}{Microbiology and Its Origins (cont)}} \tn % Row 4 \SetRowColor{LightBackground} \mymulticolumn{1}{x{8.4cm}}{What is a pure culture?} \tn \mymulticolumn{1}{x{8.4cm}}{\hspace*{6 px}\rule{2px}{6px}\hspace*{6 px}A medium used to isolated suspected bacterial pathogens} \tn % Row Count 3 (+ 3) % Row 5 \SetRowColor{white} \mymulticolumn{1}{x{8.4cm}}{Why are pure cultures important to Koch's postulates?} \tn \mymulticolumn{1}{x{8.4cm}}{\hspace*{6 px}\rule{2px}{6px}\hspace*{6 px}- To isolate suspected bacterial pathogens\{\{nl\}\} - Agar is not broken down by most bacteria\{\{nl\}\}- Agar will not melt until it reach 100c and will only solidify if it reach 50c} \tn % Row Count 9 (+ 6) \hhline{>{\arrayrulecolor{DarkBackground}}-} \end{tabularx} \par\addvspace{1.3em} % That's all folks \end{multicols*} \end{document}