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% Document Info
\author{Carsonmccall}
\pdfinfo{
  /Title (immunodiagnostics.pdf)
  /Creator (Cheatography)
  /Author (Carsonmccall)
  /Subject (Immunodiagnostics Cheat Sheet)
}

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\fancyhead[L]{
\noindent
\begin{multicols}{3}
\begin{tabulary}{5.8cm}{C}
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    \vspace{-7pt}
    {\parbox{\dimexpr\textwidth-2\fboxsep\relax}{\noindent
        \hspace*{-6pt}\includegraphics[width=5.8cm]{/web/www.cheatography.com/public/images/cheatography_logo.pdf}}
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\columnbreak
\begin{tabulary}{11cm}{L}
    \vspace{-2pt}\large{\bf{\textcolor{DarkBackground}{\textrm{Immunodiagnostics Cheat Sheet}}}} \\
    \normalsize{by \textcolor{DarkBackground}{Carsonmccall} via \textcolor{DarkBackground}{\uline{cheatography.com/209662/cs/45119/}}}
\end{tabulary}
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\fancyfoot[L]{ \footnotesize
\noindent
\begin{multicols}{3}
\begin{tabulary}{5.8cm}{LL}
  \SetRowColor{FootBackground}
  \mymulticolumn{2}{p{5.377cm}}{\bf\textcolor{white}{Cheatographer}}  \\
  \vspace{-2pt}Carsonmccall \\
  \uline{cheatography.com/carsonmccall} \\
  \end{tabulary}
\vfill
\columnbreak
\begin{tabulary}{5.8cm}{L}
  \SetRowColor{FootBackground}
  \mymulticolumn{1}{p{5.377cm}}{\bf\textcolor{white}{Cheat Sheet}}  \\
   \vspace{-2pt}Not Yet Published.\\
   Updated 28th November, 2024.\\
   Page {\thepage} of \pageref{LastPage}.
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  \vspace{-5pt}
  %\includegraphics[width=48px,height=48px]{dave.jpeg}
  Measure your website readability!\\
  www.readability-score.com
\end{tabulary}
\end{multicols}}




\begin{document}
\raggedright
\raggedcolumns

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\begin{multicols*}{3}

\begin{tabularx}{5.377cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{5.377cm}}{\bf\textcolor{white}{Immunofluorescence Assays}}  \tn
% Row 0
\SetRowColor{LightBackground}
\mymulticolumn{1}{x{5.377cm}}{} \tn 
% Row Count 0 (+ 0)
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{x{1.9908 cm} x{2.9862 cm} }
\SetRowColor{DarkBackground}
\mymulticolumn{2}{x{5.377cm}}{\bf\textcolor{white}{Fluorescent Antibody Test (FA)}}  \tn
% Row 0
\SetRowColor{LightBackground}
{\bf{Mechanism}} & Fluorescently labeled mAb to bind and illuminate a target Ag/Ab \tn 
% Row Count 3 (+ 3)
% Row 1
\SetRowColor{white}
{\bf{Designed to Detect}} & A sample's Antigen {\bf{OR}} Antibody \tn 
% Row Count 5 (+ 2)
% Row 2
\SetRowColor{LightBackground}
{\bf{Reagent}} & mAb-FITC conjugate \textasciitilde{} {\emph{Ab tagged with a Fluorescein}} \tn 
% Row Count 8 (+ 3)
% Row 3
\SetRowColor{white}
{\bf{Compatible Sample Types}} & Serum or tissue section \tn 
% Row Count 10 (+ 2)
% Row 4
\SetRowColor{LightBackground}
\mymulticolumn{2}{x{5.377cm}}{} \tn 
% Row Count 10 (+ 0)
% Row 5
\SetRowColor{white}
\mymulticolumn{2}{x{5.377cm}}{\{\{ac\}\}	 {\bf{{\emph{Types}}}}} \tn 
% Row Count 11 (+ 1)
% Row 6
\SetRowColor{LightBackground}
{\bf{1. Direct (DFA)}} & Detection of sample's {\bf{antigen}} \tn 
% Row Count 13 (+ 2)
% Row 7
\SetRowColor{white}
{\emph{Sample}} & Unknown {\bf{Antigen}} in blood \tn 
% Row Count 15 (+ 2)
% Row 8
\SetRowColor{LightBackground}
{\bf{2. Indirect (IFA)}} & Detection of sample's {\bf{antibody}} \tn 
% Row Count 17 (+ 2)
% Row 9
\SetRowColor{white}
{\emph{Sample}} & Unknown {\bf{Antibody}} in blood (Ag = known) \tn 
% Row Count 19 (+ 2)
% Row 10
\SetRowColor{LightBackground}
{\emph{Reagent}} & Secondary mAb-FITC conjugate \tn 
% Row Count 21 (+ 2)
\hhline{>{\arrayrulecolor{DarkBackground}}--}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{x{2.33919 cm} x{2.63781 cm} }
\SetRowColor{DarkBackground}
\mymulticolumn{2}{x{5.377cm}}{\bf\textcolor{white}{Direct Fluorescent Antibody}}  \tn
% Row 0
\SetRowColor{LightBackground}
\mymulticolumn{2}{x{5.377cm}}{\{\{ac\}\}	 {\bf{Uses}}} \tn 
% Row Count 1 (+ 1)
% Row 1
\SetRowColor{white}
{\emph{Bovine Viral Diarrhea Virus (BVDV)}} & Detection of {\bf{live BVDV}} in bovine blood \tn 
% Row Count 3 (+ 2)
% Row 2
\SetRowColor{LightBackground}
{\emph{Rabies in Brain Necropsy}} & Detection of the Rabies {\bf{virus}} in the brain tissue \tn 
% Row Count 6 (+ 3)
% Row 3
\SetRowColor{white}
\mymulticolumn{2}{x{5.377cm}}{} \tn 
% Row Count 6 (+ 0)
% Row 4
\SetRowColor{LightBackground}
{\bf{Sample}} & Ag from culture/slide \tn 
% Row Count 7 (+ 1)
% Row 5
\SetRowColor{white}
{\bf{Known}} & mAb-FITC conjugate against antigen of interest \tn 
% Row Count 10 (+ 3)
% Row 6
\SetRowColor{LightBackground}
{\bf{Detects (unknown reactant)}} & Antigen from sample \tn 
% Row Count 12 (+ 2)
% Row 7
\SetRowColor{white}
{\bf{Reagent}} & mAb-FITC conjugate \tn 
% Row Count 13 (+ 1)
% Row 8
\SetRowColor{LightBackground}
\mymulticolumn{2}{x{5.377cm}}{} \tn 
% Row Count 13 (+ 0)
% Row 9
\SetRowColor{white}
\mymulticolumn{2}{x{5.377cm}}{\{\{ac\}\}	 {\bf{Results}}} \tn 
% Row Count 14 (+ 1)
% Row 10
\SetRowColor{LightBackground}
{\emph{Positive Test}} & Fluorescence = Ag present \tn 
% Row Count 16 (+ 2)
% Row 11
\SetRowColor{white}
{\emph{Negative Test}} & No fluorescence = No Ag \tn 
% Row Count 18 (+ 2)
\hhline{>{\arrayrulecolor{DarkBackground}}--}
\SetRowColor{LightBackground}
\mymulticolumn{2}{x{5.377cm}}{*Only ONE 'known' Ab is used in this test \textasciitilde{} so the known test component IS the Reagent}  \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}--}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{5.377cm}}{\bf\textcolor{white}{DFA Mechanism of Action}}  \tn
\SetRowColor{LightBackground}
\mymulticolumn{1}{p{5.377cm}}{\vspace{1px}\centerline{\includegraphics[width=5.1cm]{/web/www.cheatography.com/public/uploads/carsonmccall_1732714375_IMG_2531.png}}} \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{5.377cm}}{\bf\textcolor{white}{DFA Example \textasciitilde{} BVDV}}  \tn
\SetRowColor{white}
\mymulticolumn{1}{x{5.377cm}}{1. Incubate patient serum (containing the virus) with a cultured cell line \newline % Row Count 2 (+ 2)
      \textasciitilde{} {\emph{Cell-line must be permissive to BVDV infection}} \newline % Row Count 4 (+ 2)
2. Probe with mAb-FITC conj. that targets the viral Ag of BVDV% Row Count 6 (+ 2)
} \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{5.377cm}}{\bf\textcolor{white}{BVDV DFA Results}}  \tn
\SetRowColor{LightBackground}
\mymulticolumn{1}{p{5.377cm}}{\vspace{1px}\centerline{\includegraphics[width=5.1cm]{/web/www.cheatography.com/public/uploads/carsonmccall_1732729672_Screenshot 2024-11-27 at 1.46.32 PM.png}}} \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{5.377cm}}{\bf\textcolor{white}{DFA \textasciitilde{} Rabies Brain Necropsy Dx}}  \tn
\SetRowColor{white}
\mymulticolumn{1}{x{5.377cm}}{{\bf{DFA is required for an official Rabies Dx}} \newline % Row Count 1 (+ 1)
   - An impression or tissue section of the euthanized animal's **Cerebellum, Hippocampus, and/or  \newline % Row Count 3 (+ 2)
     Brainstem** is collected  \newline % Row Count 4 (+ 1)
   - mAb-FITC targeting the Rabies virus' antigen% Row Count 5 (+ 1)
} \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{5.377cm}}{\bf\textcolor{white}{Positive DFA of Rabies in the Brain}}  \tn
\SetRowColor{LightBackground}
\mymulticolumn{1}{p{5.377cm}}{\vspace{1px}\centerline{\includegraphics[width=5.1cm]{/web/www.cheatography.com/public/uploads/carsonmccall_1732730353_Screenshot 2024-11-27 at 1.59.02 PM.png}}} \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{x{2.4885 cm} x{2.4885 cm} }
\SetRowColor{DarkBackground}
\mymulticolumn{2}{x{5.377cm}}{\bf\textcolor{white}{Indirect Fluorescent Antibody Test (IFA)}}  \tn
% Row 0
\SetRowColor{LightBackground}
\mymulticolumn{2}{x{5.377cm}}{\{\{ac\}\}	 {\bf{Uses}}} \tn 
% Row Count 1 (+ 1)
% Row 1
\SetRowColor{white}
{\emph{Porcine Reproductive and Respiratory Virus (PRRSV)}} & Detection of {\bf{PRRSV Antibody}} in Porcine serum \tn 
% Row Count 4 (+ 3)
% Row 2
\SetRowColor{LightBackground}
{\emph{Titers}} & Highest serial dilution of serum with Ab - that fluoresces \tn 
% Row Count 7 (+ 3)
% Row 3
\SetRowColor{white}
\mymulticolumn{2}{x{5.377cm}}{{\emph{Dengue Fever/ChikV/Zika Virus}}} \tn 
% Row Count 8 (+ 1)
% Row 4
\SetRowColor{LightBackground}
\mymulticolumn{2}{x{5.377cm}}{} \tn 
% Row Count 8 (+ 0)
% Row 5
\SetRowColor{white}
{\bf{Sample}} & Serum \tn 
% Row Count 9 (+ 1)
% Row 6
\SetRowColor{LightBackground}
{\bf{Known}} & Antigen \tn 
% Row Count 10 (+ 1)
% Row 7
\SetRowColor{white}
{\bf{Detects (unknown reactant)}} & Antibody from serum (1ºAb) \tn 
% Row Count 12 (+ 2)
% Row 8
\SetRowColor{LightBackground}
{\bf{Reagent}} & Anti-spp. Ab-FITC conjugate (2ºAb) \tn 
% Row Count 14 (+ 2)
% Row 9
\SetRowColor{white}
\mymulticolumn{2}{x{5.377cm}}{} \tn 
% Row Count 14 (+ 0)
% Row 10
\SetRowColor{LightBackground}
\mymulticolumn{2}{x{5.377cm}}{\{\{ac\}\}	 {\bf{Results}}} \tn 
% Row Count 15 (+ 1)
% Row 11
\SetRowColor{white}
{\emph{Positive Test}} & Fluorescence = Ab present \tn 
% Row Count 17 (+ 2)
% Row 12
\SetRowColor{LightBackground}
{\emph{Negative Test}} & No fluorescence = No Ab \tn 
% Row Count 19 (+ 2)
\hhline{>{\arrayrulecolor{DarkBackground}}--}
\SetRowColor{LightBackground}
\mymulticolumn{2}{x{5.377cm}}{*This test uses TWO antibodies \textasciitilde{} a 1º and 2º antibody}  \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}--}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{5.377cm}}{\bf\textcolor{white}{IFA Mechanism}}  \tn
\SetRowColor{LightBackground}
\mymulticolumn{1}{p{5.377cm}}{\vspace{1px}\centerline{\includegraphics[width=5.1cm]{/web/www.cheatography.com/public/uploads/carsonmccall_1732731313_Screenshot 2024-11-27 at 2.15.00 PM.png}}} \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{5.377cm}}{\bf\textcolor{white}{IFA Example \textasciitilde{} PRRSV}}  \tn
\SetRowColor{white}
\mymulticolumn{1}{x{5.377cm}}{{\bf{Detection of {\emph{Antibody}} against PRRSV in Swine serum}} \newline % Row Count 2 (+ 2)
    \textgreater{} {\bf{Known:}} PRRSV infected cell line \newline % Row Count 3 (+ 1)
    \textgreater{} {\bf{Sample:}} Porcine serum incubated with cell line \newline % Row Count 5 (+ 2)
    \textgreater{} {\bf{2ºAb Probe:}} Anti-pig IgG conjugated with FITC \newline % Row Count 7 (+ 2)
    \textgreater{} {\bf{Unknown:}} Antibody against PRRSV% Row Count 8 (+ 1)
} \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{5.377cm}}{\bf\textcolor{white}{Results of PRRSV IFA Test}}  \tn
\SetRowColor{LightBackground}
\mymulticolumn{1}{p{5.377cm}}{\vspace{1px}\centerline{\includegraphics[width=5.1cm]{/web/www.cheatography.com/public/uploads/carsonmccall_1732731679_Screenshot 2024-11-27 at 2.21.08 PM.png}}} \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{5.377cm}}{\bf\textcolor{white}{IFAs Results for DENV 1-4/CHIKV/ZIKV}}  \tn
\SetRowColor{LightBackground}
\mymulticolumn{1}{p{5.377cm}}{\vspace{1px}\centerline{\includegraphics[width=5.1cm]{/web/www.cheatography.com/public/uploads/carsonmccall_1732731762_Screenshot 2024-11-27 at 2.22.30 PM.png}}} \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{5.377cm}}{\bf\textcolor{white}{Enzyme-Linked Immunosorbent Assay (ELISA)}}  \tn
% Row 0
\SetRowColor{LightBackground}
\mymulticolumn{1}{x{5.377cm}}{} \tn 
% Row Count 0 (+ 0)
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{x{1.9908 cm} x{2.9862 cm} }
\SetRowColor{DarkBackground}
\mymulticolumn{2}{x{5.377cm}}{\bf\textcolor{white}{ELISA}}  \tn
% Row 0
\SetRowColor{LightBackground}
\mymulticolumn{2}{x{5.377cm}}{\{\{ac\}\}	 High {\emph{sensitivity}} / Low {\emph{specificity}}} \tn 
% Row Count 1 (+ 1)
% Row 1
\SetRowColor{white}
\mymulticolumn{2}{x{5.377cm}}{} \tn 
% Row Count 1 (+ 0)
% Row 2
\SetRowColor{LightBackground}
\mymulticolumn{2}{x{5.377cm}}{\{\{ac\}\}	 {\bf{Test Types that {\emph{Detect Antigen}}}}} \tn 
% Row Count 2 (+ 1)
% Row 3
\SetRowColor{white}
\textgreater{} & {\bf{Direct ELISA}} \tn 
% Row Count 3 (+ 1)
% Row 4
\SetRowColor{LightBackground}
or & {\emph{Sandwich ELISA}} \tn 
% Row Count 4 (+ 1)
% Row 5
\SetRowColor{white}
or & {\emph{Antigen Capture ELISA}} \tn 
% Row Count 5 (+ 1)
% Row 6
\SetRowColor{LightBackground}
or & {\emph{Antigen ELISA}} \tn 
% Row Count 6 (+ 1)
% Row 7
\SetRowColor{white}
\mymulticolumn{2}{x{5.377cm}}{\{\{ac\}\} {\bf{USES}}} \tn 
% Row Count 7 (+ 1)
% Row 8
\SetRowColor{LightBackground}
{\emph{Heartworm Test (HWTM)}} & Using the Anti-HTWM-Ab-HRP \tn 
% Row Count 9 (+ 2)
% Row 9
\SetRowColor{white}
\mymulticolumn{2}{x{5.377cm}}{} \tn 
% Row Count 9 (+ 0)
% Row 10
\SetRowColor{LightBackground}
\mymulticolumn{2}{x{5.377cm}}{\{\{ac\}\}	 {\bf{Test Types that {\emph{Detect Antibody}}}}} \tn 
% Row Count 10 (+ 1)
% Row 11
\SetRowColor{white}
\textgreater{} & {\bf{Indirect ELISA}} \tn 
% Row Count 11 (+ 1)
% Row 12
\SetRowColor{LightBackground}
or & {\emph{Antibody ELISA}} \tn 
% Row Count 12 (+ 1)
% Row 13
\SetRowColor{white}
\mymulticolumn{2}{x{5.377cm}}{\{\{ac\}\} {\bf{USES}}} \tn 
% Row Count 13 (+ 1)
% Row 14
\SetRowColor{LightBackground}
{\emph{Titrations (titers)}} & Quantifies the amount of Ab present \tn 
% Row Count 15 (+ 2)
\hhline{>{\arrayrulecolor{DarkBackground}}--}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{x{1.94103 cm} x{3.03597 cm} }
\SetRowColor{DarkBackground}
\mymulticolumn{2}{x{5.377cm}}{\bf\textcolor{white}{DIRECT (Ag Capture) ELISA}}  \tn
% Row 0
\SetRowColor{LightBackground}
{\bf{Sample}} & Serum from patient \tn 
% Row Count 1 (+ 1)
% Row 1
\SetRowColor{white}
{\bf{Known}} & 1º Capture Ab (coats wells in tray) \tn 
% Row Count 3 (+ 2)
% Row 2
\SetRowColor{LightBackground}
{\bf{Detection of (unknown)}} & Antigen \tn 
% Row Count 5 (+ 2)
% Row 3
\SetRowColor{white}
{\bf{Reagent + Substrate}}* & 2º Detection Ab \textasciitilde{} {\emph{specific to disease conjugated to enzyme}} \tn 
% Row Count 8 (+ 3)
% Row 4
\SetRowColor{LightBackground}
{\bf{Positive Test}} & Color change = Ag present \tn 
% Row Count 10 (+ 2)
% Row 5
\SetRowColor{white}
{\bf{Negative Test}} & No color change = No Ag \tn 
% Row Count 12 (+ 2)
\hhline{>{\arrayrulecolor{DarkBackground}}--}
\SetRowColor{LightBackground}
\mymulticolumn{2}{x{5.377cm}}{{\emph{ substrate = activates enzyme \newline }}* The capture Ab and the detection Ab may be the same Ab \textgreater{} BUT ONLY the detection Ab will be tagged with the enzyme}  \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}--}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{5.377cm}}{\bf\textcolor{white}{Ag-Capture ELISA \textasciitilde{} MOA}}  \tn
\SetRowColor{LightBackground}
\mymulticolumn{1}{p{5.377cm}}{\vspace{1px}\centerline{\includegraphics[width=5.1cm]{/web/www.cheatography.com/public/uploads/carsonmccall_1732738224_Screenshot 2024-11-27 at 4.07.36 PM.png}}} \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{5.377cm}}{\bf\textcolor{white}{Ag-Capture ELISA \textasciitilde{} MOA}}  \tn
\SetRowColor{LightBackground}
\mymulticolumn{1}{p{5.377cm}}{\vspace{1px}\centerline{\includegraphics[width=5.1cm]{/web/www.cheatography.com/public/uploads/carsonmccall_1732737827_Screenshot 2024-11-27 at 4.01.40 PM.png}}} \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{5.377cm}}{\bf\textcolor{white}{Ag-Capture ELISA \textasciitilde{} MOA}}  \tn
\SetRowColor{LightBackground}
\mymulticolumn{1}{p{5.377cm}}{\vspace{1px}\centerline{\includegraphics[width=5.1cm]{/web/www.cheatography.com/public/uploads/carsonmccall_1732739992_OSC_Microbio_20_04_ELISA1-870x1024.jpg}}} \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{5.377cm}}{\bf\textcolor{white}{Ag Capture ELISA \textasciitilde{} HTWM Ag Detection}}  \tn
\SetRowColor{white}
\mymulticolumn{1}{x{5.377cm}}{\textgreater{} {\bf{1º Capture Ab:}} Ab that targets Ab \newline % Row Count 1 (+ 1)
\textgreater{} {\bf{Sample with unknown:}} Serum with HTWM Ag \newline % Row Count 2 (+ 1)
\textgreater{} Wash slide \newline % Row Count 3 (+ 1)
\textgreater{} {\bf{2º Detection Ab:}} Anti-HTWM-Ab-HRP \newline % Row Count 4 (+ 1)
\textgreater{} Add substrate to activate enzyme to show color change if bound% Row Count 6 (+ 2)
} \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{5.377cm}}{\bf\textcolor{white}{HTWM Ag-Capture ELISA Results}}  \tn
\SetRowColor{LightBackground}
\mymulticolumn{1}{p{5.377cm}}{\vspace{1px}\centerline{\includegraphics[width=5.1cm]{/web/www.cheatography.com/public/uploads/carsonmccall_1732737786_Screenshot 2024-11-27 at 4.01.46 PM.png}}} \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{5.377cm}}{\bf\textcolor{white}{Ag-Capture ELISA \textasciitilde{} MOA}}  \tn
\SetRowColor{LightBackground}
\mymulticolumn{1}{p{5.377cm}}{\vspace{1px}\centerline{\includegraphics[width=5.1cm]{/web/www.cheatography.com/public/uploads/carsonmccall_1732738359_Screenshot 2024-11-27 at 4.12.12 PM.png}}} \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{x{1.94103 cm} x{3.03597 cm} }
\SetRowColor{DarkBackground}
\mymulticolumn{2}{x{5.377cm}}{\bf\textcolor{white}{INDIRECT (Antibody) ELISA}}  \tn
% Row 0
\SetRowColor{LightBackground}
{\bf{Sample}} & Serum from patient \tn 
% Row Count 1 (+ 1)
% Row 1
\SetRowColor{white}
{\bf{Known}} & Antigen (coating wells) \tn 
% Row Count 2 (+ 1)
% Row 2
\SetRowColor{LightBackground}
{\bf{Detection of (unknown)}} & 1º Ab in serum (spec. for Ag) \tn 
% Row Count 4 (+ 2)
% Row 3
\SetRowColor{white}
{\bf{Reagent + Substrate}}* & Anti-Ab 2º Detection Antibody-HRP conj. \textasciitilde{} *Targets hosts own Ab (1º Ab from serum) \tn 
% Row Count 8 (+ 4)
% Row 4
\SetRowColor{LightBackground}
{\bf{Positive Test}} & Color change = Ab present \tn 
% Row Count 10 (+ 2)
% Row 5
\SetRowColor{white}
{\bf{Negative Test}} & No color change = No Ab \tn 
% Row Count 12 (+ 2)
\hhline{>{\arrayrulecolor{DarkBackground}}--}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{5.377cm}}{\bf\textcolor{white}{Ab ELISA \textasciitilde{} MOA}}  \tn
\SetRowColor{LightBackground}
\mymulticolumn{1}{p{5.377cm}}{\vspace{1px}\centerline{\includegraphics[width=5.1cm]{/web/www.cheatography.com/public/uploads/carsonmccall_1732739867_Screenshot 2024-11-27 at 4.37.26 PM.png}}} \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{5.377cm}}{\bf\textcolor{white}{Ab ELISA \textasciitilde{} MOA}}  \tn
\SetRowColor{LightBackground}
\mymulticolumn{1}{p{5.377cm}}{\vspace{1px}\centerline{\includegraphics[width=5.1cm]{/web/www.cheatography.com/public/uploads/carsonmccall_1732739948_OSC_Microbio_20_04_Indirect.jpg}}} \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{p{0.4977 cm} p{0.4977 cm} }
\SetRowColor{DarkBackground}
\mymulticolumn{2}{x{5.377cm}}{\bf\textcolor{white}{Ab ELISA Titration}}  \tn
% Row 0
\SetRowColor{LightBackground}
\mymulticolumn{2}{x{5.377cm}}{} \tn 
% Row Count 0 (+ 0)
\hhline{>{\arrayrulecolor{DarkBackground}}--}
\SetRowColor{LightBackground}
\mymulticolumn{2}{x{5.377cm}}{\textgreater{} Coat ELISA wells with Ag (can get commercial Ag of interest) \newline \textgreater{} Add serial dilutions of patient's serum into wells  \newline \textgreater{} wash off unbound Ab \newline \textgreater{} Add 2º Ab like Rabbit-Anti-Horse-Ig thats conjugated w enzyme, to the wells \newline \textgreater{} wash off unbound Ab  \newline \textgreater{} add substrate}  \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}--}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{5.377cm}}{\bf\textcolor{white}{Ab ELISA \textasciitilde{} MOA}}  \tn
\SetRowColor{LightBackground}
\mymulticolumn{1}{p{5.377cm}}{\vspace{1px}\centerline{\includegraphics[width=5.1cm]{/web/www.cheatography.com/public/uploads/carsonmccall_1732743936_Screenshot 2024-11-27 at 5.44.50 PM.png}}} \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\SetRowColor{LightBackground}
\mymulticolumn{1}{x{5.377cm}}{• Can run serum from multiple patients at once \newline • Can determine titer by running serial dilutions of the serum}  \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{5.377cm}}{\bf\textcolor{white}{Indirect ELISA Ab Titer}}  \tn
\SetRowColor{LightBackground}
\mymulticolumn{1}{p{5.377cm}}{\vspace{1px}\centerline{\includegraphics[width=5.1cm]{/web/www.cheatography.com/public/uploads/carsonmccall_1732743303_Screenshot 2024-11-27 at 5.34.03 PM.png}}} \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{5.377cm}}{\bf\textcolor{white}{Western Blot (WB)}}  \tn
% Row 0
\SetRowColor{LightBackground}
\mymulticolumn{1}{x{5.377cm}}{} \tn 
% Row Count 0 (+ 0)
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{x{2.4885 cm} x{2.4885 cm} }
\SetRowColor{DarkBackground}
\mymulticolumn{2}{x{5.377cm}}{\bf\textcolor{white}{WB}}  \tn
% Row 0
\SetRowColor{LightBackground}
\mymulticolumn{2}{x{5.377cm}}{\{\{ac\}\} Higher {\emph{specificity}} than ELISA} \tn 
% Row Count 1 (+ 1)
% Row 1
\SetRowColor{white}
\mymulticolumn{2}{x{5.377cm}}{} \tn 
% Row Count 1 (+ 0)
% Row 2
\SetRowColor{LightBackground}
\mymulticolumn{2}{x{5.377cm}}{\{\{ac\}\} {\bf{Designed to ID/Detect PROTEINS:}}} \tn 
% Row Count 2 (+ 1)
% Row 3
\SetRowColor{white}
{\bf{1. Detection of {\emph{Antibody}}}} & if patients serum contains Ab against a {\emph{specific}} protein in a complex protein mixture \tn 
% Row Count 7 (+ 5)
% Row 4
\SetRowColor{LightBackground}
{\bf{2. ID specific protein {\emph{antigen}} in mix}} & Use of a known reagent Ab to the protein of interest \tn 
% Row Count 10 (+ 3)
% Row 5
\SetRowColor{white}
\mymulticolumn{2}{x{5.377cm}}{} \tn 
% Row Count 10 (+ 0)
% Row 6
\SetRowColor{LightBackground}
{\bf{MOA}} & This is a three-stage primary binding test \tn 
% Row Count 13 (+ 3)
% Row 7
\SetRowColor{white}
{\emph{Stage I}} & Electrophoresis of a protein mixture on gels so that each component is resolved into a single band \tn 
% Row Count 18 (+ 5)
% Row 8
\SetRowColor{LightBackground}
{\emph{Stage II}} & Blotting of these protein bands to an immobilizing nitrocellulose membrane \tn 
% Row Count 22 (+ 4)
% Row 9
\SetRowColor{white}
{\emph{Stage III}} & Visualization of transferred Ag by either directly or indirectly probing the membrane with Ab's \tn 
% Row Count 27 (+ 5)
% Row 10
\SetRowColor{LightBackground}
\mymulticolumn{2}{x{5.377cm}}{} \tn 
% Row Count 27 (+ 0)
% Row 11
\SetRowColor{white}
\mymulticolumn{2}{x{5.377cm}}{\{\{ac\}\} {\bf{WB Probing Methods}}} \tn 
% Row Count 28 (+ 1)
% Row 12
\SetRowColor{LightBackground}
{\emph{Direct}} & Detection of the {\bf{Protein Antigen}} \tn 
% Row Count 30 (+ 2)
\end{tabularx}
\par\addvspace{1.3em}

\vfill
\columnbreak
\begin{tabularx}{5.377cm}{x{2.4885 cm} x{2.4885 cm} }
\SetRowColor{DarkBackground}
\mymulticolumn{2}{x{5.377cm}}{\bf\textcolor{white}{WB (cont)}}  \tn
% Row 13
\SetRowColor{LightBackground}
{\emph{Indirect}} & Detection of the {\bf{Antibody}} \tn 
% Row Count 2 (+ 2)
\hhline{>{\arrayrulecolor{DarkBackground}}--}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{5.377cm}}{\bf\textcolor{white}{WB MOA}}  \tn
\SetRowColor{LightBackground}
\mymulticolumn{1}{p{5.377cm}}{\vspace{1px}\centerline{\includegraphics[width=5.1cm]{/web/www.cheatography.com/public/uploads/carsonmccall_1732756303_Screenshot 2024-11-27 at 6.42.18 PM.png}}} \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{5.377cm}}{\bf\textcolor{white}{DIRECT WB}}  \tn
\SetRowColor{LightBackground}
\mymulticolumn{1}{p{5.377cm}}{\vspace{1px}\centerline{\includegraphics[width=5.1cm]{/web/www.cheatography.com/public/uploads/carsonmccall_1732764468_Screenshot 2024-11-27 at 8.41.38 PM.png}}} \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\SetRowColor{LightBackground}
\mymulticolumn{1}{x{5.377cm}}{1. Separate out proteins by size and charge in the unknown antigen using gel  \newline      electrophoresis \newline 2.Transfer molecules to secondary matrix \newline 3.Probe with enzyme-labeled known antibody to the antigen of interest \newline 4.Wash \newline 5. Add substrate and observe change (color or light)}  \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{x{1.84149 cm} x{3.13551 cm} }
\SetRowColor{DarkBackground}
\mymulticolumn{2}{x{5.377cm}}{\bf\textcolor{white}{DIRECT WB \textasciitilde{} Bovine Spongiform Encephalopathy (BSE)}}  \tn
% Row 0
\SetRowColor{LightBackground}
{\bf{Sample}} & Serum w Ag from pt. (separated by electroph.) \tn 
% Row Count 2 (+ 2)
% Row 1
\SetRowColor{white}
\seqsplit{\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_} & Brain tissue \tn 
% Row Count 4 (+ 2)
% Row 2
\SetRowColor{LightBackground}
{\bf{Known}} & BSE specific Ab-tagged with an enzyme (reagent) \tn 
% Row Count 6 (+ 2)
% Row 3
\SetRowColor{white}
{\bf{Detects}} & BSE Prion protein {\bf{Antigen}} ({\emph{Unknown}}) \tn 
% Row Count 8 (+ 2)
% Row 4
\SetRowColor{LightBackground}
\mymulticolumn{2}{x{5.377cm}}{} \tn 
% Row Count 8 (+ 0)
% Row 5
\SetRowColor{white}
\mymulticolumn{2}{x{5.377cm}}{\{\{ac\}\} {\bf{RESULTS}}} \tn 
% Row Count 9 (+ 1)
% Row 6
\SetRowColor{LightBackground}
{\emph{Positive Test}} & Banding that match the positive band pattern = Antigen present \tn 
% Row Count 12 (+ 3)
% Row 7
\SetRowColor{white}
{\emph{Negative Test}} & Banding that match the negative band pattern = No Antigen \tn 
% Row Count 15 (+ 3)
\hhline{>{\arrayrulecolor{DarkBackground}}--}
\SetRowColor{LightBackground}
\mymulticolumn{2}{x{5.377cm}}{{\bf{Direct WB {\emph{Required}} for Dx of BSE! \textasciitilde{} Because:}}  \newline - The BSE Ag that causes a disease is a normal brain protein in Bovine that is malfunctioning because it is folded incorrectly \newline - Since this is a normal protein in the Cow brain \textasciitilde{} There is NO IMMUNE RESPONSE that will generate  \newline  \newline \textgreater{}\textgreater{}\textgreater{} THUS: We {\bf{have}} to test for the Ag since the Ab will never be produced}  \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}--}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{5.377cm}}{\bf\textcolor{white}{Direct WB BSE Results}}  \tn
\SetRowColor{LightBackground}
\mymulticolumn{1}{p{5.377cm}}{\vspace{1px}\centerline{\includegraphics[width=5.1cm]{/web/www.cheatography.com/public/uploads/}}} \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\SetRowColor{LightBackground}
\mymulticolumn{1}{x{5.377cm}}{- 3 brain preps from 3 suspect cow with brain proteins separated \newline - Abnormal BSE-specific prion protein molecules can be detected using antibodies linked to an enzyme that results in a chemical reaction  \newline - For this test a monoclonal antibody was made that recognizes BSE-specific abnormal prion protein \textgreater{}\textgreater{}\textgreater{} This antibody is a reagent antibody (tagged with an enzyme). \newline  \newline {\bf{RESULTS}} \newline Cow \#3 has BSE-specific prion proteins in its brain.}  \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{x{2.14011 cm} x{2.83689 cm} }
\SetRowColor{DarkBackground}
\mymulticolumn{2}{x{5.377cm}}{\bf\textcolor{white}{INDIRECT WB}}  \tn
% Row 0
\SetRowColor{LightBackground}
\mymulticolumn{2}{x{5.377cm}}{\{\{ac\}\} {\bf{USES}}} \tn 
% Row Count 1 (+ 1)
% Row 1
\SetRowColor{white}
{\emph{ELISA Dx Confirmation}} & Feline Immunodeficiency Virus (FIV) \tn 
% Row Count 3 (+ 2)
% Row 2
\SetRowColor{LightBackground}
 & Human Immunodeficiency Virus (HIV) \tn 
% Row Count 5 (+ 2)
% Row 3
\SetRowColor{white}
\mymulticolumn{2}{x{5.377cm}}{} \tn 
% Row Count 5 (+ 0)
% Row 4
\SetRowColor{LightBackground}
{\bf{Sample}} & Patient serum w Ab (separated by \tn 
% Row Count 7 (+ 2)
% Row 5
\SetRowColor{white}
{\bf{Known}} & HIV Ag (from known HIV-infected cells) \tn 
% Row Count 9 (+ 2)
% Row 6
\SetRowColor{LightBackground}
{\bf{Detects (unknown reactant)}} & Ab spec. to HIV Ag \tn 
% Row Count 11 (+ 2)
% Row 7
\SetRowColor{white}
{\bf{Reagent}} & Anti-spp Ab conj. to enzyme \tn 
% Row Count 13 (+ 2)
% Row 8
\SetRowColor{LightBackground}
\mymulticolumn{2}{x{5.377cm}}{} \tn 
% Row Count 13 (+ 0)
% Row 9
\SetRowColor{white}
\mymulticolumn{2}{x{5.377cm}}{\{\{ac\}\} {\bf{INTERPRETATION}}} \tn 
% Row Count 14 (+ 1)
% Row 10
\SetRowColor{LightBackground}
{\emph{Positive Test}} & Banding Pattern matches that of known positive = Ab present \tn 
% Row Count 17 (+ 3)
% Row 11
\SetRowColor{white}
{\emph{Negative Test}} & Banding Pattern matches that of known negative = No Ab \tn 
% Row Count 20 (+ 3)
\hhline{>{\arrayrulecolor{DarkBackground}}--}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{5.377cm}}{\bf\textcolor{white}{INDIRECT WB MOA}}  \tn
\SetRowColor{LightBackground}
\mymulticolumn{1}{p{5.377cm}}{\vspace{1px}\centerline{\includegraphics[width=5.1cm]{/web/www.cheatography.com/public/uploads/carsonmccall_1732765952_Screenshot 2024-11-27 at 8.41.50 PM.png}}} \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\SetRowColor{LightBackground}
\mymulticolumn{1}{x{5.377cm}}{1. Separate out proteins by size and charge in the known antigen using gel electrophoresis \newline 2.Transfer molecules to secondary matrix \newline 3.Probe with patient's serum antibody \newline 4.Add enzyme-labeled antibody to patient's antibody \newline 5.Wash \newline 6. Add substrate and observe change (color or light)}  \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{x{1.94103 cm} x{3.03597 cm} }
\SetRowColor{DarkBackground}
\mymulticolumn{2}{x{5.377cm}}{\bf\textcolor{white}{INDIRECT WB \textasciitilde{} FIV Confirmation}}  \tn
% Row 0
\SetRowColor{LightBackground}
{\bf{Source for Ag}} & Proteins from FIV-infected cells (separated out by electrophoresis) \tn 
% Row Count 3 (+ 3)
% Row 1
\SetRowColor{white}
{\bf{Sample with unknown Ab}} & Cat's serum w/ Ab \tn 
% Row Count 5 (+ 2)
% Row 2
\SetRowColor{LightBackground}
{\bf{Detection Reagent}} & Anti-cat Ab conju. w enzyme \tn 
% Row Count 7 (+ 2)
\hhline{>{\arrayrulecolor{DarkBackground}}--}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{5.377cm}}{\bf\textcolor{white}{Immunohistochemistry (IHC)}}  \tn
% Row 0
\SetRowColor{LightBackground}
\mymulticolumn{1}{x{5.377cm}}{} \tn 
% Row Count 0 (+ 0)
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{x{1.89126 cm} x{3.08574 cm} }
\SetRowColor{DarkBackground}
\mymulticolumn{2}{x{5.377cm}}{\bf\textcolor{white}{IHC Test}}  \tn
% Row 0
\SetRowColor{LightBackground}
\mymulticolumn{2}{x{5.377cm}}{\{\{ac\}\} {\bf{{\emph{- Always detects antigen}}}}} \tn 
% Row Count 1 (+ 1)
% Row 1
\SetRowColor{white}
\mymulticolumn{2}{x{5.377cm}}{\{\{ac\}\} {\emph{- Horseradish peroxidase (brown color)}}} \tn 
% Row Count 2 (+ 1)
% Row 2
\SetRowColor{LightBackground}
\mymulticolumn{2}{x{5.377cm}}{\{\{ac\}\} {\bf{{\emph{- (-) Controls =}} irrelevant Ab {\bf{OR}} normal tissue section}}} \tn 
% Row Count 4 (+ 2)
% Row 3
\SetRowColor{white}
\mymulticolumn{2}{x{5.377cm}}{} \tn 
% Row Count 4 (+ 0)
% Row 4
\SetRowColor{LightBackground}
{\bf{Sample}} & Thin tissue section \tn 
% Row Count 5 (+ 1)
% Row 5
\SetRowColor{white}
{\bf{Known \textasciitilde{} 1º reagent}} & 1º Reagent Antibody \textasciitilde{} probes Ag \tn 
% Row Count 7 (+ 2)
% Row 6
\SetRowColor{LightBackground}
{\bf{Detects (unknown)}} & Antigen in the Tissue sect. \tn 
% Row Count 9 (+ 2)
% Row 7
\SetRowColor{white}
{\bf{2º Detection reagent}} & 2º Detection Ab conj. \textasciitilde{} spec. for 1º Ab \tn 
% Row Count 11 (+ 2)
% Row 8
\SetRowColor{LightBackground}
\mymulticolumn{2}{x{5.377cm}}{} \tn 
% Row Count 11 (+ 0)
% Row 9
\SetRowColor{white}
\mymulticolumn{2}{x{5.377cm}}{\{\{ac\}\} {\bf{CONTROLS}}} \tn 
% Row Count 12 (+ 1)
% Row 10
\SetRowColor{LightBackground}
\mymulticolumn{2}{x{5.377cm}}{\{\{ac\}\} {\emph{Positive Control}}} \tn 
% Row Count 13 (+ 1)
% Row 11
\SetRowColor{white}
{\bf{1º Reagent}} & 1º Reagent Ab \textasciitilde{} spec. for tissue Ag \tn 
% Row Count 15 (+ 2)
% Row 12
\SetRowColor{LightBackground}
{\bf{Positive Result}} & Brown in color = Ag is present \tn 
% Row Count 17 (+ 2)
% Row 13
\SetRowColor{white}
\mymulticolumn{2}{x{5.377cm}}{} \tn 
% Row Count 17 (+ 0)
% Row 14
\SetRowColor{LightBackground}
\mymulticolumn{2}{x{5.377cm}}{\{\{ac\}\} {\emph{Negative Control}}} \tn 
% Row Count 18 (+ 1)
% Row 15
\SetRowColor{white}
{\bf{1º Reagent}} & 1º Reagent Ab \textasciitilde{} spec. for tissue Ag {\bf{NOT IN SAMPLE}} \tn 
% Row Count 21 (+ 3)
% Row 16
\SetRowColor{LightBackground}
{\bf{Negative Result}} & No color change = No Ag \tn 
% Row Count 23 (+ 2)
\hhline{>{\arrayrulecolor{DarkBackground}}--}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{5.377cm}}{\bf\textcolor{white}{IHC MOA}}  \tn
\SetRowColor{LightBackground}
\mymulticolumn{1}{p{5.377cm}}{\vspace{1px}\centerline{\includegraphics[width=5.1cm]{/web/www.cheatography.com/public/uploads/carsonmccall_1732804944_Screenshot 2024-11-28 at 10.41.53 AM.png}}} \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{5.377cm}}{\bf\textcolor{white}{IHC Microscopy Result}}  \tn
\SetRowColor{LightBackground}
\mymulticolumn{1}{p{5.377cm}}{\vspace{1px}\centerline{\includegraphics[width=5.1cm]{/web/www.cheatography.com/public/uploads/carsonmccall_1732804898_Screenshot 2024-11-28 at 10.41.11 AM.png}}} \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{x{2.18988 cm} x{2.78712 cm} }
\SetRowColor{DarkBackground}
\mymulticolumn{2}{x{5.377cm}}{\bf\textcolor{white}{IHC Negative Control}}  \tn
% Row 0
\SetRowColor{LightBackground}
\mymulticolumn{2}{x{5.377cm}}{\{\{ac\}\} {\bf{USES}}} \tn 
% Row Count 1 (+ 1)
% Row 1
\SetRowColor{white}
{\emph{Detection of mammary tumor Ag}}* & Use of an irrelevant rabbit mAb spec. to tumor Ag \tn 
% Row Count 4 (+ 3)
% Row 2
\SetRowColor{LightBackground}
\mymulticolumn{2}{x{5.377cm}}{{\emph{Detection of {\bf{Brucella Melitensis}}}}} \tn 
% Row Count 5 (+ 1)
\hhline{>{\arrayrulecolor{DarkBackground}}--}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{x{1.64241 cm} x{3.33459 cm} }
\SetRowColor{DarkBackground}
\mymulticolumn{2}{x{5.377cm}}{\bf\textcolor{white}{IHC \textasciitilde{} Brucella Melitensis}}  \tn
% Row 0
\SetRowColor{LightBackground}
{\bf{Sample}} & Histo section of a Goat's tissue from the Prepuce of the Penis and the Seminal Vesicular Gland \tn 
% Row Count 4 (+ 4)
% Row 1
\SetRowColor{white}
{\bf{1º Reagent Ab}} & mAb spec. to {\emph{B. melitensis}} Ag \tn 
% Row Count 6 (+ 2)
% Row 2
\SetRowColor{LightBackground}
{\bf{2º Detection Ab}} & 2º mAb spec. to 1º Ab \tn 
% Row Count 8 (+ 2)
% Row 3
\SetRowColor{white}
\mymulticolumn{2}{x{5.377cm}}{} \tn 
% Row Count 8 (+ 0)
% Row 4
\SetRowColor{LightBackground}
\mymulticolumn{2}{x{5.377cm}}{\{\{ac\}\} {\bf{RESULTS}}} \tn 
% Row Count 9 (+ 1)
% Row 5
\SetRowColor{white}
{\emph{Positive Test}} & Observed brown color when compared to controls = {\bf{Ag present}} \tn 
% Row Count 12 (+ 3)
% Row 6
\SetRowColor{LightBackground}
{\emph{Negative Test}} & No color change from controls = {\bf{No Ag}} \tn 
% Row Count 14 (+ 2)
\hhline{>{\arrayrulecolor{DarkBackground}}--}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{5.377cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{5.377cm}}{\bf\textcolor{white}{{\emph{B. Melitensis}} IHC Results}}  \tn
\SetRowColor{LightBackground}
\mymulticolumn{1}{p{5.377cm}}{\vspace{1px}\centerline{\includegraphics[width=5.1cm]{/web/www.cheatography.com/public/uploads/carsonmccall_1732806525_Screenshot 2024-11-28 at 10.55.25 AM.png}}} \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\SetRowColor{LightBackground}
\mymulticolumn{1}{x{5.377cm}}{Top: Mucosal epithelium of the Prepuce of the Penis \newline Bottom: Seminal Vesicular Gland epithelia}  \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}


% That's all folks
\end{multicols*}

\end{document}