\documentclass[10pt,a4paper]{article}

% Packages
\usepackage{fancyhdr}           % For header and footer
\usepackage{multicol}           % Allows multicols in tables
\usepackage{tabularx}           % Intelligent column widths
\usepackage{tabulary}           % Used in header and footer
\usepackage{hhline}             % Border under tables
\usepackage{graphicx}           % For images
\usepackage{xcolor}             % For hex colours
%\usepackage[utf8x]{inputenc}    % For unicode character support
\usepackage[T1]{fontenc}        % Without this we get weird character replacements
\usepackage{colortbl}           % For coloured tables
\usepackage{setspace}           % For line height
\usepackage{lastpage}           % Needed for total page number
\usepackage{seqsplit}           % Splits long words.
%\usepackage{opensans}          % Can't make this work so far. Shame. Would be lovely.
\usepackage[normalem]{ulem}     % For underlining links
% Most of the following are not required for the majority
% of cheat sheets but are needed for some symbol support.
\usepackage{amsmath}            % Symbols
\usepackage{MnSymbol}           % Symbols
\usepackage{wasysym}            % Symbols
%\usepackage[english,german,french,spanish,italian]{babel}              % Languages

% Document Info
\author{ACD Marketing Team (ACD Marketing)}
\pdfinfo{
  /Title (viral-pathogen-research.pdf)
  /Creator (Cheatography)
  /Author (ACD Marketing Team (ACD Marketing))
  /Subject (Viral Pathogen Research Cheat Sheet)
}

% Lengths and widths
\addtolength{\textwidth}{6cm}
\addtolength{\textheight}{-1cm}
\addtolength{\hoffset}{-3cm}
\addtolength{\voffset}{-2cm}
\setlength{\tabcolsep}{0.2cm} % Space between columns
\setlength{\headsep}{-12pt} % Reduce space between header and content
\setlength{\headheight}{85pt} % If less, LaTeX automatically increases it
\renewcommand{\footrulewidth}{0pt} % Remove footer line
\renewcommand{\headrulewidth}{0pt} % Remove header line
\renewcommand{\seqinsert}{\ifmmode\allowbreak\else\-\fi} % Hyphens in seqsplit
% This two commands together give roughly
% the right line height in the tables
\renewcommand{\arraystretch}{1.3}
\onehalfspacing

% Commands
\newcommand{\SetRowColor}[1]{\noalign{\gdef\RowColorName{#1}}\rowcolor{\RowColorName}} % Shortcut for row colour
\newcommand{\mymulticolumn}[3]{\multicolumn{#1}{>{\columncolor{\RowColorName}}#2}{#3}} % For coloured multi-cols
\newcolumntype{x}[1]{>{\raggedright}p{#1}} % New column types for ragged-right paragraph columns
\newcommand{\tn}{\tabularnewline} % Required as custom column type in use

% Font and Colours
\definecolor{HeadBackground}{HTML}{333333}
\definecolor{FootBackground}{HTML}{666666}
\definecolor{TextColor}{HTML}{333333}
\definecolor{DarkBackground}{HTML}{0090A8}
\definecolor{LightBackground}{HTML}{EFF8F9}
\renewcommand{\familydefault}{\sfdefault}
\color{TextColor}

% Header and Footer
\pagestyle{fancy}
\fancyhead{} % Set header to blank
\fancyfoot{} % Set footer to blank
\fancyhead[L]{
\noindent
\begin{multicols}{3}
\begin{tabulary}{5.8cm}{C}
    \SetRowColor{DarkBackground}
    \vspace{-7pt}
    {\parbox{\dimexpr\textwidth-2\fboxsep\relax}{\noindent
        \hspace*{-6pt}\includegraphics[width=5.8cm]{/web/www.cheatography.com/public/images/cheatography_logo.pdf}}
    }
\end{tabulary}
\columnbreak
\begin{tabulary}{11cm}{L}
    \vspace{-2pt}\large{\bf{\textcolor{DarkBackground}{\textrm{Viral Pathogen Research Cheat Sheet}}}} \\
    \normalsize{by \textcolor{DarkBackground}{ACD Marketing Team (ACD Marketing)} via \textcolor{DarkBackground}{\uline{cheatography.com/36805/cs/11583/}}}
\end{tabulary}
\end{multicols}}

\fancyfoot[L]{ \footnotesize
\noindent
\begin{multicols}{3}
\begin{tabulary}{5.8cm}{LL}
  \SetRowColor{FootBackground}
  \mymulticolumn{2}{p{5.377cm}}{\bf\textcolor{white}{Cheatographer}}  \\
  \vspace{-2pt}ACD Marketing Team (ACD Marketing) \\
  \uline{cheatography.com/acd-marketing} \\
        \uline{\seqsplit{www}.acdbio.com}
  \end{tabulary}
\vfill
\columnbreak
\begin{tabulary}{5.8cm}{L}
  \SetRowColor{FootBackground}
  \mymulticolumn{1}{p{5.377cm}}{\bf\textcolor{white}{Cheat Sheet}}  \\
   \vspace{-2pt}Not Yet Published.\\
   Updated 28th April, 2017.\\
   Page {\thepage} of \pageref{LastPage}.
\end{tabulary}
\vfill
\columnbreak
\begin{tabulary}{5.8cm}{L}
  \SetRowColor{FootBackground}
  \mymulticolumn{1}{p{5.377cm}}{\bf\textcolor{white}{Sponsor}}  \\
  \SetRowColor{white}
  \vspace{-5pt}
  %\includegraphics[width=48px,height=48px]{dave.jpeg}
  Measure your website readability!\\
  www.readability-score.com
\end{tabulary}
\end{multicols}}




\begin{document}
\raggedright
\raggedcolumns

% Set font size to small. Switch to any value
% from this page to resize cheat sheet text:
% www.emerson.emory.edu/services/latex/latex_169.html
\footnotesize % Small font.

\begin{multicols*}{2}

\begin{tabularx}{8.4cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{8.4cm}}{\bf\textcolor{white}{One Minute Pitch}}  \tn
\SetRowColor{white}
\mymulticolumn{1}{x{8.4cm}}{RNAscope ISH detects viral RNA, provides context, is highly sensitive and strand-specific; therefore delivers {\bf{where}} the {\bf{actual virus}} is  and  {\bf{what viral state it is in}} (resting, replicating). Assay can be applied to {\bf{any sequenced virus, }} already applied to \textgreater{}100 different viruses.% Row Count 6 (+ 6)
} \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{8.4cm}{x{2.8 cm} x{5.2 cm} }
\SetRowColor{DarkBackground}
\mymulticolumn{2}{x{8.4cm}}{\bf\textcolor{white}{Pain Points \& Our Solutions}}  \tn
% Row 0
\SetRowColor{LightBackground}
{\bf{Viral Location}} & {\bf{Context}} provides viral localization which answers where virus entered, replicate, cause disease, shed \tn 
% Row Count 5 (+ 5)
% Row 1
\SetRowColor{white}
{\bf{No Antibody }} & {\bf{Any Gene}} of any sequenced virus, or for any gene in a virus. \tn 
% Row Count 8 (+ 3)
% Row 2
\SetRowColor{LightBackground}
{\bf{Antibody Surrogate}} & {\bf{Direct}} measurement of the actual virus not a protein that is produced as a host response \tn 
% Row Count 12 (+ 4)
% Row 3
\SetRowColor{white}
{\bf{Low viremia}} & {\bf{High sensitivity}} to detect single RNA molecules, proven for early infections and HIV/SIV reservoirs \tn 
% Row Count 16 (+ 4)
% Row 4
\SetRowColor{LightBackground}
{\bf{Emerging Viruses}} & {\bf{Rapid}} design and assay enable researchers to respond to emerging viral investigations \tn 
% Row Count 20 (+ 4)
% Row 5
\SetRowColor{white}
{\bf{Viral State}} & {\bf{Strand-specific}} probes discern viral stages; hybridize to sense (+) or anti-sense (-) strand to detect replication, resting stages \tn 
% Row Count 26 (+ 6)
% Row 6
\SetRowColor{LightBackground}
{\bf{Viral Causality}} & {\bf{Localization \& viral state}} are needed to determine whether virus is just present or actually causing the disease \tn 
% Row Count 31 (+ 5)
\end{tabularx}
\par\addvspace{1.3em}

\vfill
\columnbreak
\begin{tabularx}{8.4cm}{x{2.8 cm} x{5.2 cm} }
\SetRowColor{DarkBackground}
\mymulticolumn{2}{x{8.4cm}}{\bf\textcolor{white}{Pain Points \& Our Solutions (cont)}}  \tn
% Row 7
\SetRowColor{LightBackground}
{\bf{Uncommon species}} & {\bf{Any species}} with a sequence. We have probes for viruses that infects raccoon, seal, fox \tn 
% Row Count 4 (+ 4)
% Row 8
\SetRowColor{white}
{\bf{RNA/DNA}} & {\bf{RNAscope and/or DNAscope}} to visualize viral states. \tn 
% Row Count 7 (+ 3)
\hhline{>{\arrayrulecolor{DarkBackground}}--}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{8.4cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{8.4cm}}{\bf\textcolor{white}{Solution for DNA \& Retroviruses-{}-DNAscope ISH}}  \tn
\SetRowColor{white}
\mymulticolumn{1}{x{8.4cm}}{\{\{link="https://acdbio.com/innovations-rnascope"\}\} DNAscope ISH  \{\{/link\}\} is a customer proven protocol. It can be used to detect viral DNA using a non-standard, modified protocol.  It can be combined with RNA ISH so you see both viral RNA \& DNA   \newline % Row Count 5 (+ 5)
\{\{link="https://paijournal.com/index.php/paijournal/article/view/100/43"\}\} Defining HIV and SIV Reservoirs in Lymphoid Tissues.\{\{/link\}\}  \newline % Row Count 8 (+ 3)
{\bf{Non-standard, can be cumbersome, recommend with caution}}% Row Count 10 (+ 2)
} \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{8.4cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{8.4cm}}{\bf\textcolor{white}{Key Tools / References}}  \tn
\SetRowColor{white}
\mymulticolumn{1}{x{8.4cm}}{{\bf{URLs}}   https://acdbio.com/zika    https://acdbio.com/HIV    \newline % Row Count 2 (+ 2)
\seqsplit{https://acdbio.com/infectiousdiseases}  \newline % Row Count 3 (+ 1)
\{\{link="https://acdbio.com/innovations"\}\}DNAscope Information\{\{/link\}\} \newline % Row Count 5 (+ 2)
{\bf{2 Spotlight Interviews}} \{\{link="https://acdbio.com/researcherspotlight"\}\}Pesavento \&  Getu and Smits \{\{/link\}\} (Showpad) \newline % Row Count 8 (+ 3)
{\bf{Presentation}} Viral Pathogen Comprehensive PPT (Showpad) \newline % Row Count 10 (+ 2)
{\bf{Data}} Loads of publications \& data images in PPT mentioned above \newline % Row Count 12 (+ 2)
{\bf{Who}} McCune Lab at UCSF for HIV, US CDC for Zika,% Row Count 14 (+ 2)
} \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{8.4cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{8.4cm}}{\bf\textcolor{white}{Research Goals}}  \tn
% Row 0
\SetRowColor{LightBackground}
\mymulticolumn{1}{x{8.4cm}}{{\bf{Viral pathogenesis}} how biological viruses cause diseases in their target hosts, Pathogenesis is a process in which an initial infection becomes a disease} \tn 
% Row Count 4 (+ 4)
% Row 1
\SetRowColor{white}
\mymulticolumn{1}{x{8.4cm}}{{\bf{Viral immunity}} correlates of immunity to infection and the development of novel approaches to the diagnosis, prevention and treatment} \tn 
% Row Count 7 (+ 3)
% Row 2
\SetRowColor{LightBackground}
\mymulticolumn{1}{x{8.4cm}}{{\bf{Viral emergence}} mechanisms that underpin host switching, examine same virus in multiple host species} \tn 
% Row Count 10 (+ 3)
% Row 3
\SetRowColor{white}
\mymulticolumn{1}{x{8.4cm}}{{\bf{Viral spread}} examine viral spread between livestock and wildlife. Examine resevoirs of infection and routes of transmission} \tn 
% Row Count 13 (+ 3)
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{8.4cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{8.4cm}}{\bf\textcolor{white}{Virus and Hosts}}  \tn
\SetRowColor{LightBackground}
\mymulticolumn{1}{p{8.4cm}}{\vspace{1px}\centerline{\includegraphics[width=5.1cm]{/web/www.cheatography.com/public/uploads/acd-marketing_1493319702_VirusInfectious_ecologyEvolution.jpg}}} \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\SetRowColor{LightBackground}
\mymulticolumn{1}{x{8.4cm}}{What is the viral type \& viral group? Are there closely related species? \newline What is the host species and is there more than one? Is the parthenogenesis specific to the host?}  \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{8.4cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{8.4cm}}{\bf\textcolor{white}{Qualifying Questions}}  \tn
% Row 0
\SetRowColor{LightBackground}
\mymulticolumn{1}{x{8.4cm}}{Is your goal to locate the virus, find what state it is in or both?} \tn 
\mymulticolumn{1}{x{8.4cm}}{\hspace*{6 px}\rule{2px}{6px}\hspace*{6 px}RNAscope ISH can provide location and viral state info} \tn 
% Row Count 4 (+ 4)
% Row 1
\SetRowColor{white}
\mymulticolumn{1}{x{8.4cm}}{Which specific viral replication state do you want to detect? Attachment/Penetration/ \seqsplit{Uncoating/Replication/Assembly/Release}?} \tn 
\mymulticolumn{1}{x{8.4cm}}{\hspace*{6 px}\rule{2px}{6px}\hspace*{6 px}For that specific viral state is it + /- stranded-{}-probes designed complementary to that strand.} \tn 
% Row Count 10 (+ 6)
% Row 2
\SetRowColor{LightBackground}
\mymulticolumn{1}{x{8.4cm}}{Is the antibody available for viral protein or is it a surrogate marker?} \tn 
\mymulticolumn{1}{x{8.4cm}}{\hspace*{6 px}\rule{2px}{6px}\hspace*{6 px}RNAscope hybridize to the actual viral RNA and is strand specific, so it measures the actual virus in viral state.} \tn 
% Row Count 15 (+ 5)
% Row 3
\SetRowColor{white}
\mymulticolumn{1}{x{8.4cm}}{Is low viremia a concern-{}-early infection or viral reservoirs?} \tn 
\mymulticolumn{1}{x{8.4cm}}{\hspace*{6 px}\rule{2px}{6px}\hspace*{6 px}Single molecule detection is proven with RNAscope. Several RNAscope HIV/SIV resevoir papers available.} \tn 
% Row Count 20 (+ 5)
% Row 4
\SetRowColor{LightBackground}
\mymulticolumn{1}{x{8.4cm}}{Do you need to link a viral stage to a disease? Do you want to detect a viral RNA and a disease marker?} \tn 
\mymulticolumn{1}{x{8.4cm}}{\hspace*{6 px}\rule{2px}{6px}\hspace*{6 px}Multiplexing options available, so one probe can be design for the virus and another for the disease marker} \tn 
% Row Count 26 (+ 6)
% Row 5
\SetRowColor{white}
\mymulticolumn{1}{x{8.4cm}}{Is host switching a concern?} \tn 
\mymulticolumn{1}{x{8.4cm}}{\hspace*{6 px}\rule{2px}{6px}\hspace*{6 px}If the virus is the same in both hosts, then we can use one probe. But if the virus evolves from host to host, our probes are specific and be designed to the sequence} \tn 
% Row Count 31 (+ 5)
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{8.4cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{8.4cm}}{\bf\textcolor{white}{Background-{}- Compatible with all 7 Viral Groups}}  \tn
\SetRowColor{LightBackground}
\mymulticolumn{1}{p{8.4cm}}{\vspace{1px}\centerline{\includegraphics[width=5.1cm]{/web/www.cheatography.com/public/uploads/acd-marketing_1493207079_VirologyImage.jpg}}} \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\SetRowColor{LightBackground}
\mymulticolumn{1}{x{8.4cm}}{Publications using RNAscope ISH can be found for 6/7 viral groups}  \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{8.4cm}{x{3.12 cm} x{4.88 cm} }
\SetRowColor{DarkBackground}
\mymulticolumn{2}{x{8.4cm}}{\bf\textcolor{white}{Background-{}-Concepts \& Vocabulary}}  \tn
% Row 0
\SetRowColor{LightBackground}
Tropism & viral specificity for host tissue/cell (viral surface structures \& host surface receptors) \tn 
% Row Count 4 (+ 4)
% Row 1
\SetRowColor{white}
Dynamics & speed of viral progression within host \tn 
% Row Count 6 (+ 2)
% Row 2
\SetRowColor{LightBackground}
Load/ Burden & quantity of virus in a given volume \tn 
% Row Count 8 (+ 2)
% Row 3
\SetRowColor{white}
Latency & ability of \seqsplit{a pathogenic virus to} lie dormant within a cell \tn 
% Row Count 11 (+ 3)
% Row 4
\SetRowColor{LightBackground}
Reservoir & cell type or anatomical site where (resting/latent) virus can hide, stay stable for future replication. May also be used to describe species that harbour the virus without causing disease. \tn 
% Row Count 19 (+ 8)
% Row 5
\SetRowColor{white}
Virions & virus while not inside an infected cell or in the process of infecting a cell \tn 
% Row Count 23 (+ 4)
% Row 6
\SetRowColor{LightBackground}
3 viral types & DNA, RNA (most common) \& Retrovirus \tn 
% Row Count 25 (+ 2)
% Row 7
\SetRowColor{white}
7 viral groups & see chart \tn 
% Row Count 26 (+ 1)
% Row 8
\SetRowColor{LightBackground}
Sense (+) anti-sense virus & Sense virus hybridize with anti-sense probes, anti-sense virus hybridize with sense probes \tn 
% Row Count 30 (+ 4)
\end{tabularx}
\par\addvspace{1.3em}

\vfill
\columnbreak
\begin{tabularx}{8.4cm}{x{3.12 cm} x{4.88 cm} }
\SetRowColor{DarkBackground}
\mymulticolumn{2}{x{8.4cm}}{\bf\textcolor{white}{Background-{}-Concepts \& Vocabulary (cont)}}  \tn
% Row 9
\SetRowColor{LightBackground}
Ambi-sense strand virus & single stranded genome with both +- strands requiring both sense \& anti-sense probes \tn 
% Row Count 4 (+ 4)
% Row 10
\SetRowColor{white}
Host switching & species jumping, cross species transmission-{}-a virus infects a new host species and may have changed at molecular level...new strain (SARS, Ebola, HIV) \tn 
% Row Count 11 (+ 7)
\hhline{>{\arrayrulecolor{DarkBackground}}--}
\end{tabularx}
\par\addvspace{1.3em}


% That's all folks
\end{multicols*}

\end{document}