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% Document Info
\author{ACD Marketing Team (ACD Marketing)}
\pdfinfo{
  /Title (nucleic-acid-therapeutics.pdf)
  /Creator (Cheatography)
  /Author (ACD Marketing Team (ACD Marketing))
  /Subject (Nucleic Acid Therapeutics Cheat Sheet)
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    \vspace{-2pt}\large{\bf{\textcolor{DarkBackground}{\textrm{Nucleic Acid Therapeutics Cheat Sheet}}}} \\
    \normalsize{by \textcolor{DarkBackground}{ACD Marketing Team (ACD Marketing)} via \textcolor{DarkBackground}{\uline{cheatography.com/36805/cs/11597/}}}
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  \mymulticolumn{2}{p{5.377cm}}{\bf\textcolor{white}{Cheatographer}}  \\
  \vspace{-2pt}ACD Marketing Team (ACD Marketing) \\
  \uline{cheatography.com/acd-marketing} \\
        \uline{\seqsplit{www}.acdbio.com}
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  \mymulticolumn{1}{p{5.377cm}}{\bf\textcolor{white}{Cheat Sheet}}  \\
   \vspace{-2pt}Not Yet Published.\\
   Updated 2nd June, 2017.\\
   Page {\thepage} of \pageref{LastPage}.
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  Measure your website readability!\\
  www.readability-score.com
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\begin{multicols*}{2}

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\mymulticolumn{1}{x{8.4cm}}{\bf\textcolor{white}{One minute pitch}}  \tn
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\mymulticolumn{1}{x{8.4cm}}{RNAscope® and BaseScope™ are ideal solutions to detect therapeutic or target gene expression and vector sequences in individual cells within the tissue morphological context, addressing critical questions on the efficacy and safety behind nucleic acid based therapeutics.% Row Count 6 (+ 6)
} \tn 
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\mymulticolumn{1}{x{8.4cm}}{\bf\textcolor{white}{Therapy and RNAscope application}}  \tn
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\mymulticolumn{1}{x{8.4cm}}{Nucleic Acid Therapeutics includes 3 main categories of therapy: \newline % Row Count 2 (+ 2)
{\bf{Gene Therapy}}: insertion of genetically altered genes into cells to replace defective genes. Use viral or non-viral vector.  \newline % Row Count 5 (+ 3)
{\emph{RNAscope Technology is used to detect vector distribution and viability, and transgene expression in tissue}}. See publication P0187, P0643, P0693 \newline % Row Count 8 (+ 3)
{\bf{RNA Therapy}}: Direct delivery of mRNA (altered or not) into cells to replace defective gene expression.  \newline % Row Count 11 (+ 3)
{\emph{RNAscope technology is used to detect mRNA expression in tissue}}. See publications P0504, P0783 \newline % Row Count 13 (+ 2)
{\bf{RNAi Therapy}}: use of siRNA or miRNA to silence or turn off gene expression. Note that a viral vector can be used to transduct the siRNA/miRNA. \newline % Row Count 16 (+ 3)
{\emph{RNAscope is used to measure impact of the RNAi on targeted gene expression}}. See publications P0420, P0357, P0575, P0481% Row Count 19 (+ 3)
} \tn 
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\mymulticolumn{1}{x{8.4cm}}{\bf\textcolor{white}{CRISPR/Cas9}}  \tn
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\mymulticolumn{1}{x{8.4cm}}{When applied to gene therapy, CRISPR/Cas9 can be used in 2 ways: \newline % Row Count 2 (+ 2)
{\bf{ex vivo gene correction}}: modification of target ex vivo before reintroduction in the body (example: CAR-T cell). RNAscope technology is used to localize and monitor expression of the modified target in tissue. \newline % Row Count 7 (+ 5)
{\bf{in vivo gene correction}}: introduction of CRISPR/Cas9 system in tissue/body for modification in vivo. RNAscope technology could be used to localize and monitor guide RNA and expression of the modified target in tissue.% Row Count 12 (+ 5)
} \tn 
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\mymulticolumn{1}{x{8.4cm}}{\bf\textcolor{white}{Gene Therapy: AAV transgene and detection}}  \tn
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\mymulticolumn{1}{p{8.4cm}}{\vspace{1px}\centerline{\includegraphics[width=5.1cm]{/web/www.cheatography.com/public/uploads/acd-marketing_1496335000_Image for gene therapy.png}}} \tn 
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\mymulticolumn{1}{x{8.4cm}}{RNAscope provides the unique capacity to localize and measure AAV vector expression at the single cell level in tissue. Not feasible with IHC.}  \tn 
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\mymulticolumn{1}{x{8.4cm}}{\bf\textcolor{white}{Question to ask and solution}}  \tn
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\mymulticolumn{1}{x{8.4cm}}{How do you verify that the vector TARGETs the right cells and right gene?} \tn 
\mymulticolumn{1}{x{8.4cm}}{\hspace*{6 px}\rule{2px}{6px}\hspace*{6 px}{\bf{RNAscope}}: Visualize in vivo delivery of therapeutic gene expression and vector sequences (viral or non-viral) with tissue specificity, cell type specificity and subcellular localization. {\bf{Multiplex ISH or dual ISH-IHC}}: localize target (ISH) and identify cell type (ISH or IHC) if needed.} \tn 
% Row Count 9 (+ 9)
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\mymulticolumn{1}{x{8.4cm}}{How do you verify the transgene gets ACTIVATED?} \tn 
\mymulticolumn{1}{x{8.4cm}}{\hspace*{6 px}\rule{2px}{6px}\hspace*{6 px}{\bf{RNAscope}}: visualize and measure transgene expression over time with high sensitivity and specificity.} \tn 
% Row Count 13 (+ 4)
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\mymulticolumn{1}{x{8.4cm}}{How do you measure the EFFICIENCY of the therapy?} \tn 
\mymulticolumn{1}{x{8.4cm}}{\hspace*{6 px}\rule{2px}{6px}\hspace*{6 px}{\bf{RNAscope}}: Allows quantification of vector copy \#, transgene expression level, and AAV+ cell number in both target and non-target tissues} \tn 
% Row Count 17 (+ 4)
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\mymulticolumn{1}{x{8.4cm}}{Which animal models do you use or plan to use for safety testing?} \tn 
\mymulticolumn{1}{x{8.4cm}}{\hspace*{6 px}\rule{2px}{6px}\hspace*{6 px}{\bf{ RNAscope}}: an assay for any target and any species and any tissue - allows to easily measure expression of vector and target in different animal models and tissues.} \tn 
% Row Count 23 (+ 6)
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\mymulticolumn{1}{x{8.4cm}}{\bf\textcolor{white}{Key tools/References}}  \tn
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\mymulticolumn{1}{x{8.4cm}}{\{\{link="http://bit.ly/2stdHu6"\}\}Gene Therapy Flyer\{\{/link\}\}  \newline % Row Count 2 (+ 2)
\{\{link="http://bit.ly/2rYoXSy"\}\}Pubcrush and presentation\{\{/link\}\}  \newline % Row Count 4 (+ 2)
\{\{link="http://bit.ly/2sjgoiG"\}\}Leads and Web sites\{\{/link\}\} \newline % Row Count 6 (+ 2)
\{\{link="http://bit.ly/2rwrK4J"\}\}Publication List\{\{/link\}\} \newline % Row Count 8 (+ 2)
\{\{link="http://bit.ly/2qGFo1L"\}\}email template\{\{/link\}\}% Row Count 10 (+ 2)
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\mymulticolumn{1}{x{8.4cm}}{\bf\textcolor{white}{Recommanded products}}  \tn
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\mymulticolumn{1}{x{8.4cm}}{Both the RNAscope \& BaseScope Assays are relevant for this application. Nevertheless, RNAscope will cover the majority of the needs.% Row Count 3 (+ 3)
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\mymulticolumn{1}{x{8.4cm}}{\bf\textcolor{white}{Targeted Species, Tissues \& Diseases}}  \tn
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\mymulticolumn{1}{x{8.4cm}}{\bf\textcolor{white}{List of target}}  \tn
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\mymulticolumn{1}{x{8.4cm}}{{\bf{Gene Therapy}}: AAV vector and targeted gene (specific to the disease) \newline % Row Count 2 (+ 2)
{\bf{RNA Therapy}}: Targeted gene (specific to disease) \newline % Row Count 4 (+ 2)
{\bf{miRNA Therapy}}: Targeted gene (specific to disease)% Row Count 6 (+ 2)
} \tn 
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% That's all folks
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