\documentclass[10pt,a4paper]{article}

% Packages
\usepackage{fancyhdr}           % For header and footer
\usepackage{multicol}           % Allows multicols in tables
\usepackage{tabularx}           % Intelligent column widths
\usepackage{tabulary}           % Used in header and footer
\usepackage{hhline}             % Border under tables
\usepackage{graphicx}           % For images
\usepackage{xcolor}             % For hex colours
%\usepackage[utf8x]{inputenc}    % For unicode character support
\usepackage[T1]{fontenc}        % Without this we get weird character replacements
\usepackage{colortbl}           % For coloured tables
\usepackage{setspace}           % For line height
\usepackage{lastpage}           % Needed for total page number
\usepackage{seqsplit}           % Splits long words.
%\usepackage{opensans}          % Can't make this work so far. Shame. Would be lovely.
\usepackage[normalem]{ulem}     % For underlining links
% Most of the following are not required for the majority
% of cheat sheets but are needed for some symbol support.
\usepackage{amsmath}            % Symbols
\usepackage{MnSymbol}           % Symbols
\usepackage{wasysym}            % Symbols
%\usepackage[english,german,french,spanish,italian]{babel}              % Languages

% Document Info
\author{ACD Marketing Team (ACD Marketing)}
\pdfinfo{
  /Title (neuroscience.pdf)
  /Creator (Cheatography)
  /Author (ACD Marketing Team (ACD Marketing))
  /Subject (Neuroscience Cheat Sheet)
}

% Lengths and widths
\addtolength{\textwidth}{6cm}
\addtolength{\textheight}{-1cm}
\addtolength{\hoffset}{-3cm}
\addtolength{\voffset}{-2cm}
\setlength{\tabcolsep}{0.2cm} % Space between columns
\setlength{\headsep}{-12pt} % Reduce space between header and content
\setlength{\headheight}{85pt} % If less, LaTeX automatically increases it
\renewcommand{\footrulewidth}{0pt} % Remove footer line
\renewcommand{\headrulewidth}{0pt} % Remove header line
\renewcommand{\seqinsert}{\ifmmode\allowbreak\else\-\fi} % Hyphens in seqsplit
% This two commands together give roughly
% the right line height in the tables
\renewcommand{\arraystretch}{1.3}
\onehalfspacing

% Commands
\newcommand{\SetRowColor}[1]{\noalign{\gdef\RowColorName{#1}}\rowcolor{\RowColorName}} % Shortcut for row colour
\newcommand{\mymulticolumn}[3]{\multicolumn{#1}{>{\columncolor{\RowColorName}}#2}{#3}} % For coloured multi-cols
\newcolumntype{x}[1]{>{\raggedright}p{#1}} % New column types for ragged-right paragraph columns
\newcommand{\tn}{\tabularnewline} % Required as custom column type in use

% Font and Colours
\definecolor{HeadBackground}{HTML}{333333}
\definecolor{FootBackground}{HTML}{666666}
\definecolor{TextColor}{HTML}{333333}
\definecolor{DarkBackground}{HTML}{005A9B}
\definecolor{LightBackground}{HTML}{EFF4F8}
\renewcommand{\familydefault}{\sfdefault}
\color{TextColor}

% Header and Footer
\pagestyle{fancy}
\fancyhead{} % Set header to blank
\fancyfoot{} % Set footer to blank
\fancyhead[L]{
\noindent
\begin{multicols}{3}
\begin{tabulary}{5.8cm}{C}
    \SetRowColor{DarkBackground}
    \vspace{-7pt}
    {\parbox{\dimexpr\textwidth-2\fboxsep\relax}{\noindent
        \hspace*{-6pt}\includegraphics[width=5.8cm]{/web/www.cheatography.com/public/images/cheatography_logo.pdf}}
    }
\end{tabulary}
\columnbreak
\begin{tabulary}{11cm}{L}
    \vspace{-2pt}\large{\bf{\textcolor{DarkBackground}{\textrm{Neuroscience Cheat Sheet}}}} \\
    \normalsize{by \textcolor{DarkBackground}{ACD Marketing Team (ACD Marketing)} via \textcolor{DarkBackground}{\uline{cheatography.com/36805/cs/11592/}}}
\end{tabulary}
\end{multicols}}

\fancyfoot[L]{ \footnotesize
\noindent
\begin{multicols}{3}
\begin{tabulary}{5.8cm}{LL}
  \SetRowColor{FootBackground}
  \mymulticolumn{2}{p{5.377cm}}{\bf\textcolor{white}{Cheatographer}}  \\
  \vspace{-2pt}ACD Marketing Team (ACD Marketing) \\
  \uline{cheatography.com/acd-marketing} \\
        \uline{\seqsplit{www}.acdbio.com}
  \end{tabulary}
\vfill
\columnbreak
\begin{tabulary}{5.8cm}{L}
  \SetRowColor{FootBackground}
  \mymulticolumn{1}{p{5.377cm}}{\bf\textcolor{white}{Cheat Sheet}}  \\
   \vspace{-2pt}Not Yet Published.\\
   Updated 28th April, 2017.\\
   Page {\thepage} of \pageref{LastPage}.
\end{tabulary}
\vfill
\columnbreak
\begin{tabulary}{5.8cm}{L}
  \SetRowColor{FootBackground}
  \mymulticolumn{1}{p{5.377cm}}{\bf\textcolor{white}{Sponsor}}  \\
  \SetRowColor{white}
  \vspace{-5pt}
  %\includegraphics[width=48px,height=48px]{dave.jpeg}
  Measure your website readability!\\
  www.readability-score.com
\end{tabulary}
\end{multicols}}




\begin{document}
\raggedright
\raggedcolumns

% Set font size to small. Switch to any value
% from this page to resize cheat sheet text:
% www.emerson.emory.edu/services/latex/latex_169.html
\footnotesize % Small font.

\begin{multicols*}{2}

\begin{tabularx}{8.4cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{8.4cm}}{\bf\textcolor{white}{One Minute Pitch}}  \tn
\SetRowColor{white}
\mymulticolumn{1}{x{8.4cm}}{RNAscope® ISH delivers single-molecule sensitivity and specificity. The {\bf{multiplexing capability}} enables detection of multiple markers at once, enabling exquisite characterization of cell populations, {\bf{co-localization/ co-expression}} studies and also {\bf{sub-cellular localization}} of mRNA within the nervous system. Combine with {\bf{Immunohistochemistry/ IF}}. {\bf{Same-day}} results and automated assays mean you can get the data you seek quickly, easily, and all backed by our performance guarantee.% Row Count 11 (+ 11)
} \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{8.4cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{8.4cm}}{\bf\textcolor{white}{Key Selling Point}}  \tn
\SetRowColor{white}
\mymulticolumn{1}{x{8.4cm}}{{\bf{Multiplex capability and dual ISH/IHC}}% Row Count 1 (+ 1)
} \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{8.4cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{8.4cm}}{\bf\textcolor{white}{Overview}}  \tn
\SetRowColor{LightBackground}
\mymulticolumn{1}{p{8.4cm}}{\vspace{1px}\centerline{\includegraphics[width=5.1cm]{/web/www.cheatography.com/public/uploads/acd-marketing_1493297607_Overview.png}}} \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\SetRowColor{LightBackground}
\mymulticolumn{1}{x{8.4cm}}{{\bf{No Antibody?}} Detect GPCRs, Ion Channels, lncRNA \newline {\bf{Validate RNA-Seq Signatures}} in single cells \newline {\bf{Visualize cellular activity in cell type specific manner}} \newline Plasticity markers, Secreted factors, Co-localization \newline {\bf{Characterize specific genetic variations}} \newline Alternative splicing, circRNA}  \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{8.4cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{8.4cm}}{\bf\textcolor{white}{Qualifying Questions}}  \tn
% Row 0
\SetRowColor{LightBackground}
\mymulticolumn{1}{x{8.4cm}}{Do you want to explore molecular mechanisms of CNS/PNS development and neurological disorders like Alzheimer, Parkinson, Addictions, Pain, Depression...?} \tn 
% Row Count 4 (+ 4)
% Row 1
\SetRowColor{white}
\mymulticolumn{1}{x{8.4cm}}{Do you need to visualize biomarkers co-localization \& expression in specific cellular sub-types?} \tn 
% Row Count 6 (+ 2)
% Row 2
\SetRowColor{LightBackground}
\mymulticolumn{1}{x{8.4cm}}{Are you looking for single cell resolution or need to distinguish expression of biomarkers between various nervous system cell types?} \tn 
% Row Count 9 (+ 3)
% Row 3
\SetRowColor{white}
\mymulticolumn{1}{x{8.4cm}}{Would you like to visualize sub cellular RNA/protein localization, axon/dendrites RNA transport?} \tn 
% Row Count 11 (+ 2)
% Row 4
\SetRowColor{LightBackground}
\mymulticolumn{1}{x{8.4cm}}{Are you investigating expression of splice variants (\textasciitilde{}90\% of genes in the CNS have splice variants involved in specific diseases)?} \tn 
% Row Count 14 (+ 3)
% Row 5
\SetRowColor{white}
\mymulticolumn{1}{x{8.4cm}}{Do you need an assay for a non-commun target like for circRNA?} \tn 
% Row Count 16 (+ 2)
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{8.4cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{8.4cm}}{\bf\textcolor{white}{Pain Points and Our Solutions}}  \tn
% Row 0
\SetRowColor{LightBackground}
\mymulticolumn{1}{x{8.4cm}}{{\bf{Immunohistochemistry}}\{\{nl\}\} {\bf{Pain Point:}} Lack of \seqsplit{antibody/specificity/secreted} proteins/ lncRNA \{\{nl\}\}{\bf{Our Solution:}} Detection of any gene/ species/ tissue with highest specificity, new probe in 2 weeks, multiplex – up to 4 RNAs + 2 IHC targets. Co-expression studies with cell type markers, sub-cellular localization possible, detection of GPCRs/ Ion Channels} \tn 
% Row Count 8 (+ 8)
% Row 1
\SetRowColor{white}
\mymulticolumn{1}{x{8.4cm}}{{\bf{Conventional ISH}}\{\{nl\}\} {\bf{Paint Point:}} Considered as complicated, long assay, dual ISH/IHC possible but really tricky
\{\{nl\}\}{\bf{Our Solution:}} One day assay (not V2), easy-to-use, stable assay, great tech support (new user program), probe in depended optimization} \tn 
% Row Count 14 (+ 6)
% Row 2
\SetRowColor{LightBackground}
\mymulticolumn{1}{x{8.4cm}}{{\bf{RNA-Seq/ RT-PCR}}\{\{nl\}\} {\bf{Pain Point:}} Give up tissue context, quantity of RNA required for qPCR is higher
\{\{nl\}\}{\bf{Our Solution:}} Morphological context with single-cell specificity} \tn 
% Row Count 18 (+ 4)
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{8.4cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{8.4cm}}{\bf\textcolor{white}{Recommended Products}}  \tn
% Row 0
\SetRowColor{LightBackground}
\mymulticolumn{1}{x{8.4cm}}{{\bf{RNAscope® Fluorescent Multiplex}} (320850) for fresh \& fixed frozen \{\{nl\}\}C1/C3: stronger channel (low expressor), C2: slightly weaker channel \{\{nl\}\}} \tn 
% Row Count 4 (+ 4)
% Row 1
\SetRowColor{white}
\mymulticolumn{1}{x{8.4cm}}{{\bf{RNAscope® Multiplex Fluorescent v2}} (323110) up to 4 targets, but ideal for FFPE, 4-plex: They need 4-Plex Ancillary Kit (323120)} \tn 
% Row Count 7 (+ 3)
% Row 2
\SetRowColor{LightBackground}
\mymulticolumn{1}{x{8.4cm}}{{\bf{RNAscope® 2.5 HD Duplex Reagent Kit}} (322430)} \tn 
% Row Count 8 (+ 1)
% Row 3
\SetRowColor{white}
\mymulticolumn{1}{x{8.4cm}}{{\bf{BaseScope}} for splice variants, circRNA} \tn 
% Row Count 9 (+ 1)
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{8.4cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{8.4cm}}{\bf\textcolor{white}{Our Limitations}}  \tn
% Row 0
\SetRowColor{LightBackground}
\mymulticolumn{1}{x{8.4cm}}{{\bf{Multiplexing}}= With V2 6 Opal fluorophores available \{\{nl\}\}(Theoretically 4x RNA, 2x protein as we only have 4 channels)} \tn 
% Row Count 3 (+ 3)
% Row 1
\SetRowColor{white}
\mymulticolumn{1}{x{8.4cm}}{{\bf{ISH/IF}}= We can not guarantee results as depending on the antibody.} \tn 
% Row Count 5 (+ 2)
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{8.4cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{8.4cm}}{\bf\textcolor{white}{Most Published Targets:}}  \tn
% Row 0
\SetRowColor{LightBackground}
\mymulticolumn{1}{x{8.4cm}}{Gad1, Drd2, Drd1, VGluT2, Gad2, Th, Sst, EGFP, Shh, Vgat} \tn 
% Row Count 2 (+ 2)
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{8.4cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{8.4cm}}{\bf\textcolor{white}{Long non-coding RNA in Neuroscience - examples}}  \tn
\SetRowColor{white}
\mymulticolumn{1}{x{8.4cm}}{Alzheimer's disease (BACE1-AS, SORL1, lncRNA 51A…)\{\{nl\}\}Schizophrenia (Gomafu, DISC2…)\{\{nl\}\}Autism spectrum disorder (MSNP1AS)\{\{nl\}\}Parkinson's disease (AL049437, AK021630...)\{\{nl\}\}Huntington's disease (Neat1)% Row Count 5 (+ 5)
} \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}

\begin{tabularx}{8.4cm}{X}
\SetRowColor{DarkBackground}
\mymulticolumn{1}{x{8.4cm}}{\bf\textcolor{white}{Key Tools/ References:}}  \tn
\SetRowColor{white}
\mymulticolumn{1}{x{8.4cm}}{App Note, App Review, Probe List, Publication List - http://bit.ly/2oN1lum \newline % Row Count 2 (+ 2)
GPCR Video - \seqsplit{https://www.youtube.com/watch?v=SFjUVZGdToc} \newline % Row Count 4 (+ 2)
Several PubCrushs/ scientific posters - please check Showpad% Row Count 6 (+ 2)
} \tn 
\hhline{>{\arrayrulecolor{DarkBackground}}-}
\end{tabularx}
\par\addvspace{1.3em}


% That's all folks
\end{multicols*}

\end{document}